Method for preparing xylo-oligosaccharide syrup and powdered sugar by using complex enzyme

Abstract

The invention relates to a method for preparing xylo-oligosaccharide syrup and powdered sugar by using a complex enzyme. The method comprises the following steps of: (1) crushing a cellulosic raw material and preparing the cellulosic raw material into a suspension; (2) adjusting the pH value of the suspension to be between 5.5 and 6.5, adding a high temperature-resistant alpha-amylase into the suspension, after raising the temperature to 85 to 95 DEG C, hydrolyzing the suspension, cooling the suspension, adjusting the pH value to be between 7.5 and 8.5, adding an alkali protease, stirring the suspension at a constant speed, after raising the temperature to 45 to 60 DEG C, hydrolyzing the suspension, and inactivating the enzyme; (3) centrifuging the suspension in which the enzyme is inactivated to precipitate, and washing and drying the deposit; (4) preparing the deposit into a dietary fiber suspension, adjusting the pH value to 5.6, and adding the liquid complex enzyme consisting of incision beta-1,4-xylanase, feruloyl esterase and fungal laccase into the suspension to obtain a xylo-oligosaccharide enzymatic hydrolysate; and (5) performing precise filtration, nano filtration, super filtration, desalination, decoloration and concentration on the xylo-oligosaccharide enzymatic hydrolysate to obtain the xylo-oligosaccharide syrup. The method is simple in process and wide in raw material source; the raw materials can be mixed; the production time is shortened; the production efficiency and the yield are improved.

Description

technical field [0001] The invention belongs to the field of xylo-oligosaccharide preparation, in particular to a method for preparing xylo-oligosaccharide syrup and powdered sugar by using compound enzymes. Background technique [0002] Xylo-oligosaccharide, also known as xylo-oligosaccharide, is a mixture of 2-7 xylose molecules combined with β-1,4 glycosidic bonds, with xylobiose and xylotriose as the main components. Xylo-oligosaccharide is an important functional food. In addition to its good physical and chemical characteristics such as low heat, stability, safety and non-toxicity, it also has the unique physiological properties of promoting the reproduction of probiotic flora in the intestinal tract and inhibiting the growth of harmful flora. It can be widely used in food, beverage, medical care and other fields. [0003] At present, there are two main methods for the production of xylo-oligosaccharides: acid degradation and biodegradation. When the acid degradation...

AI Technical Summary

Problems solved by technology

Because the purification of microbial fermentation products is very difficult, the current industrial production of xylo-oligosaccharides mainly uses enzymatic hydrolysis, and the existing enzymatic hydrolysis mainly uses a single enzyme for hydrolysis, and the corresponding raw material is also a single type, such as a single corn cob , bagasse, straw, wheat bran, cottonseed hulls or rapeseed hulls, the types of raw materials used in the preparation are limited, and the enzymatic hydrolysis takes a long time, usually 16-24 hours, and the yield of xylooligosaccharides is low. Only 15-20%