Method for preparing xylo-oligosaccharide syrup and powdered sugar by using complex enzyme

A technology of xylooligosaccharides and compound enzymes, which is applied in the field of sugar powder and compound enzymes to prepare xylooligosaccharide syrups, can solve the problems of limited use of raw materials, low yield of xylooligosaccharides, and long enzymatic hydrolysis time. Achieve the effects of improving production efficiency and yield, short enzymolysis time and simple process

Inactive Publication Date: 2012-07-25
TIANJIN MODERN VOCATIONAL TECH COLLEGE
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the purification of microbial fermentation products is very difficult, the current industrial production of xylo-oligosaccharides mainly uses enzymatic hydrolysis, and the existing enzymatic hydrolysis mainly uses a single enzyme for hydrolysis, and the corresponding raw material is also a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method of utilizing compound enzyme to prepare xylo-oligosaccharide syrup, the steps of the method are:

[0024] (1), the cellulose raw material is pulverized, the cellulose raw material adopted in the present embodiment is the mixture of corncob, bagasse, straw, after the pulverization, the water of 5 times of the weight of the mixture is added after 40 mesh sieve cleaning and impurity removal, and is configured as Cellulosic raw material suspension; Cellulosic raw material can also be wheat bran, cottonseed husk and rapeseed husk;

[0025] (2), using disodium hydrogen phosphate-citric acid buffer solution to adjust the pH value of the cellulose raw material suspension to 5.5, adding a high temperature resistant α-amylase of 0.5% by weight of the cellulose raw material suspension, and warming up to 0.5% under the uniform stirring condition of the mixer After hydrolysis at 85°C for 30min; cooling to 40°C, using disodium hydrogen phosphate-citric acid buffer solution t...

Embodiment 2

[0031] A method of utilizing compound enzyme to prepare xylo-oligosaccharide syrup, the steps of the method are:

[0032] (1), the cellulose raw material is pulverized, this cellulose raw material is the mixture of wheat bran, cottonseed husk and rapeseed husk, after the pulverization, 60 mesh sieves are cleaned and the water of 15 times of the mixture weight is added after the impurity removal, and configured into fiber Raw material suspension;

[0033] (2), using disodium hydrogen phosphate-citric acid buffer solution to adjust the pH value of the cellulose raw material suspension to 6.5, adding a high temperature resistant α-amylase of 1.5% by weight of the cellulose raw material suspension, and warming up to After hydrolysis at 95°C for 45min; cooled to 45°C, using disodium hydrogen phosphate-citric acid buffer solution to adjust the pH value of the cellulose raw material suspension to 8.5, adding alkaline protease with 3.5% by weight of the cellulose raw material suspensi...

Embodiment 3

[0039] A method of utilizing compound enzyme to prepare xylo-oligosaccharide syrup, the steps of the method are:

[0040] (1), cellulose raw material is pulverized, the present embodiment cellulose raw material is corncob, and after the pulverization, the water of 7 times of corncob weight is added after 50 mesh sieve cleaning and impurity removal, and is configured into cellulose raw material suspension;

[0041](2), adopt disodium hydrogen phosphate-citric acid buffer solution to adjust the pH value of the cellulose raw material suspension to 6, add the high temperature resistant α-amylase of 1% by weight of the cellulose raw material suspension, and be warming up to 1% under the uniform stirring condition of the mixer After hydrolysis at 90°C for 38min; cooled to 43°C, using disodium hydrogen phosphate-citric acid buffer solution to adjust the pH value of the cellulose raw material suspension to 8, adding alkaline protease with 3% by weight of the cellulose raw material susp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for preparing xylo-oligosaccharide syrup and powdered sugar by using a complex enzyme. The method comprises the following steps of: (1) crushing a cellulosic raw material and preparing the cellulosic raw material into a suspension; (2) adjusting the pH value of the suspension to be between 5.5 and 6.5, adding a high temperature-resistant alpha-amylase into the suspension, after raising the temperature to 85 to 95 DEG C, hydrolyzing the suspension, cooling the suspension, adjusting the pH value to be between 7.5 and 8.5, adding an alkali protease, stirring the suspension at a constant speed, after raising the temperature to 45 to 60 DEG C, hydrolyzing the suspension, and inactivating the enzyme; (3) centrifuging the suspension in which the enzyme is inactivated to precipitate, and washing and drying the deposit; (4) preparing the deposit into a dietary fiber suspension, adjusting the pH value to 5.6, and adding the liquid complex enzyme consisting of incision beta-1,4-xylanase, feruloyl esterase and fungal laccase into the suspension to obtain a xylo-oligosaccharide enzymatic hydrolysate; and (5) performing precise filtration, nano filtration, super filtration, desalination, decoloration and concentration on the xylo-oligosaccharide enzymatic hydrolysate to obtain the xylo-oligosaccharide syrup. The method is simple in process and wide in raw material source; the raw materials can be mixed; the production time is shortened; the production efficiency and the yield are improved.

Description

technical field [0001] The invention belongs to the field of xylo-oligosaccharide preparation, in particular to a method for preparing xylo-oligosaccharide syrup and powdered sugar by using compound enzymes. Background technique [0002] Xylo-oligosaccharide, also known as xylo-oligosaccharide, is a mixture of 2-7 xylose molecules combined with β-1,4 glycosidic bonds, with xylobiose and xylotriose as the main components. Xylo-oligosaccharide is an important functional food. In addition to its good physical and chemical characteristics such as low heat, stability, safety and non-toxicity, it also has the unique physiological properties of promoting the reproduction of probiotic flora in the intestinal tract and inhibiting the growth of harmful flora. It can be widely used in food, beverage, medical care and other fields. [0003] At present, there are two main methods for the production of xylo-oligosaccharides: acid degradation and biodegradation. When the acid degradation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/14
Inventor 孙勇民岳鹍刘鹏王芃王立晖刘宽范树生
Owner TIANJIN MODERN VOCATIONAL TECH COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap