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Enzymolysis method of wood fiber raw materials

A lignocellulosic raw material and enzymatic hydrolysis technology, which is applied in the field of enzymatic hydrolysis of lignocellulosic raw materials, can solve problems such as restricting the wide application of enzymatic hydrolysis methods and low enzymatic hydrolysis efficiency, and achieve mild reaction conditions, broad application prospects and cost reduction. Effect

Inactive Publication Date: 2012-02-29
GUANGZHOU CHEM CO LTD CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen from the above examples that the current efficiency of enzymatic hydrolysis is very low, and has not yet reached 50%, which greatly restricts the wide application of enzymatic hydrolysis methods, and there is an urgent need for a method to improve the efficiency of enzymatic hydrolysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] a. Take 20g of corn stalks that have been dried and crushed to 80 mesh, add 300mL of 1% sodium hydroxide solution, mix well, treat at 100°C for 3 hours, filter while hot, and wash with hot water until neutral. The filter residue is dried and crushed for later use.

[0020] b. Take 1.5g of the material obtained in step a, mix it with 70mL of newly prepared citric acid-sodium citrate buffer solution with a pH of 4.8, preheat at 50°C for 10 minutes, add 0.07g of powdered activated carbon, 0.225g of Tween -80 and 75 mg of cellulase were continuously shaken in a constant temperature oscillator at 50° C. for 45 hours, the total amount of reducing sugar was measured to be 1.185 g, and the enzymatic hydrolysis yield was 71.1%.

[0021] Enzyme hydrolysis yield = -0.9 × 100% × total amount of reducing sugar / dry weight of raw materials

Embodiment 2

[0031] a. Take 20g of corn stalks that have been dried and crushed to 80 mesh, add 300mL of 1% sodium hydroxide solution, mix well, treat at 100°C for 3 hours, filter while hot, and wash with hot water until neutral. The filter residue is dried and crushed for later use.

[0032] b. Take 1.5g of the material obtained in step a, mix it with 70mL of newly prepared citric acid-sodium citrate buffer solution with a pH of 4.8, preheat at 50°C for 10 minutes, add 0.03g of powdered activated carbon, 0.25g of Tween -20 and 30 mg of cellulase were continuously shaken in a constant temperature oscillator at 50° C. for 80 hours, the total amount of reducing sugar was measured to be 0.63 g, and the enzymatic hydrolysis yield was 37.8%.

Embodiment 3

[0038] a. Take 20g of corn stalks that have been dried and crushed to 80 mesh, add 300mL of 20% sodium hydroxide solution, mix well, treat at 100°C for 3 hours, filter while hot, and wash with hot water until neutral. The filter residue is dried and crushed for later use.

[0039] b. Take 1.5g of the material obtained in step a, mix it with 45mL of newly prepared acetic acid-sodium acetate buffer solution with a pH of 4.5, preheat at 50°C for 45 minutes, add 0.7g of powdered activated carbon, 0.12g of Tween- 80 and 75 mg of cellulase were continuously shaken in a constant temperature shaker at 55°C for 57 hours, the total amount of reducing sugar was measured to be 0.762 g, and the enzymatic hydrolysis yield was 46%.

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PUM

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Abstract

The invention discloses an enzymolysis method of wood fiber raw materials, comprising the following steps: first preprocessing the raw materials by alkali, then adding buffer solution for pre-heating and hydrolyzing the fiber raw materials by cellulase, active carbon and Tween. The method features high productivity and low cost, and has no toxic action on ecosystems and has broad application prospect.

Description

technical field [0001] The invention relates to the field of materials, in particular to an enzymatic hydrolysis method for lignocellulosic raw materials. Background technique [0002] At present, the energy resources are becoming increasingly tense, and the natural cellulose resources are attracting more and more attention. The main components of natural cellulose are cellulose, hemicellulose and lignin, of which cellulose and hemicellulose account for about 70%, which can produce sugars such as glucose and xylose after hydrolysis. The cellulose hydrolysis process mainly includes acid hydrolysis and enzymatic hydrolysis. Compared with the acid hydrolysis process, enzymatic hydrolysis has the advantages of mild reaction conditions, less by-products, and less harm to the environment. However, the main problem of enzymatic hydrolysis is the enzymatic hydrolysis. The efficiency is low, resulting in a higher cost of using the enzyme. Publication No. CN 1141281C patent "Hemicel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 廖兵刘伟庞浩计红果
Owner GUANGZHOU CHEM CO LTD CHINESE ACADEMY OF SCI
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