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Method for efficiently saccharifying straw pulp fiber

A kind of fiber and straw pulp technology, which is applied in the field of high-efficiency saccharification of straw pulp fibers, can solve the problems of high energy consumption in mechanical treatment methods, equipment corrosion engineering costs, and increased production costs, so as to reduce degradation losses, reduce equipment investment, and increase production costs. The effect of raw material yield

Active Publication Date: 2012-12-12
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem is that the energy consumption of the mechanical treatment method is very high, which undoubtedly increases the production cost
[0017] Acid hydrolysis has a history of nearly 100 years. Up to now, there are still many problems, such as acid recovery, equipment corrosion, project cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In an appropriate reaction kettle, add 400 grams of chopped air-dried corn stalks, then add hot sodium hydroxide solution at a solid-to-liquid ratio of 1:7 (weight ratio), with a lye concentration of 2%, and seal the mouth Afterwards, heat at 85°C for 1.5 hours for pretreatment, and the obtained slurry is centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor is treated with a membrane to recover the alkali; the obtained solid phase is added to the container, and water is added to adjust the concentration to 8 %, adjust the pH to 4.8 with acid, adjust the temperature to 50 ° C, add cellulase in the feed liquid, the amount of enzyme used is 25FPIU / gram substrate (on a dry basis), the stirring speed is 100rpm / min, and the enzymolysis time is controlled In 72 hours, take a sample once every 24 hours, utilize the DNS method to measure the total sugar concentration in the enzymolysis solution, utilize high-performance liquid chromatography to measure the glucos...

Embodiment 2

[0038] In an appropriate reaction kettle, add 400 grams of chopped air-dried corn stalks, then add hot sodium hydroxide solution at a solid-to-liquid ratio of 1:7 (weight ratio), with a lye concentration of 2%, and seal the mouth Afterwards, heat at 90°C for 1.5 hours for pretreatment, and the obtained slurry is centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor is treated with a membrane to recover the alkali; the obtained solid phase is added to the container, and water is added to adjust the concentration to 8 %, adjust the pH to 4.8 with acid, adjust the temperature to 50°C, add cellulase to the feed liquid, the enzyme amount is 25FPIU / gram substrate (on a dry basis), the stirring speed is 100rpm / min, and the enzymolysis time is controlled In 72 hours, take a sample once every 24 hours, utilize the DNS method to measure the total sugar concentration in the enzymolysis solution, utilize high performance liquid chromatography to measure the glucose and xylose...

Embodiment 3

[0040] In an appropriate reaction kettle, add 1200 grams of chopped air-dried corn stalks, then add hot sodium hydroxide solution at a solid-to-liquid ratio of 1:7 (weight ratio), with a lye concentration of 2%, and seal the mouth Afterwards, heat at 95°C for 1.5 hours for pretreatment, and the obtained slurry is centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor is treated with a membrane to recover the alkali; the obtained solid phase is added to the container, and water is added to adjust the concentration to 8 %, adjust the pH to 4.8 with acid, adjust the temperature to 50°C, add cellulase to the feed liquid, the enzyme amount is 25FPIU / gram substrate (on a dry basis), the stirring speed is 100rpm / min, and the enzymolysis time is controlled In 72 hours, take a sample once every 24 hours, utilize the DNS method to measure the total sugar concentration in the enzymolysis solution, utilize high-performance liquid chromatography to measure the glucose and xyl...

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Abstract

The invention relates to a method for efficiently saccharifying straw pulp fiber, comprising pre-treatment of straw pulp fiber and enzymolysis, wherein in the pre-treatment process, straw pulp fiber and aqueous alkali at a concentration of 0.1wt%-50wt% are mixed to be treated at the temperature of 70 DEG C-140 DEG C for 10-400 min; the weight ratio of the straw pulp fiber to the aqueous alkali is1:1-20.The method for efficiently saccharifying straw pulp fiber of the invention produces no harmful substance to enzymolysis and fermentation, reduces degradation loss of carbohydrate in biomass, improves raw material enzymolysis yield and effectively lowers production cost.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a method for efficiently saccharifying straw pulp fibers. Background technique [0002] Petroleum resources, which are the backbone of the world economy, are expected to be exhausted in a few decades. Therefore, the development and research of petroleum substitutes is imminent. At present, many countries are studying the preparation of fuel ethanol by bioconversion method using woody biological resources as raw materials to replace or partially replace petroleum with limited reserves. [0003] The main components of woody biomass resources are cellulose, hemicellulose and lignin. Among them, cellulose and hemicellulose are the source of fermentable sugar, and the content accounts for 66-75% (the absolute dry weight of the fibrous raw material). It is a very mature process to produce ethanol from hexose through the fermentation of Saccharomyces cerevisiae. When cellulase is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 李荣杰薛培俭尚海涛黄之文
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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