A method for improving the enzymatic hydrolysis yield of alkaline pretreated lignocellulosic raw materials

A lignocellulosic raw material and pretreatment technology, applied in the direction of fermentation, etc., can solve the problems of complicated process and unsuitable for large-scale industrial production, and achieve the effects of simple process conditions, weakened non-specific adsorption, and low energy consumption

Active Publication Date: 2019-09-10
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process is too complicated and not suitable for large-scale industrial production, so it is necessary to simplify the process of polyethoxy grafting modified lignin to improve the yield of cellulose enzymatic hydrolysis

Method used

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  • A method for improving the enzymatic hydrolysis yield of alkaline pretreated lignocellulosic raw materials
  • A method for improving the enzymatic hydrolysis yield of alkaline pretreated lignocellulosic raw materials
  • A method for improving the enzymatic hydrolysis yield of alkaline pretreated lignocellulosic raw materials

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Embodiment 1

[0026] A method for improving the enzymatic hydrolysis yield of alkaline pretreated wood fiber raw materials, the steps are as follows:

[0027] 1) Weigh 100g of absolutely dry corn stalks and use 10%, 20%, 30%, 40% (g / g, the same below) sodium hydroxide, and the solid-to-liquid ratio 1:10 (g / mL, the same below) ), the amount of polyethylene glycol diglycidyl ether is 10% (g / g, the same below), and the pretreatment is performed at 70°C for 3 hours.

[0028] 2) After the pretreatment, the materials are collected by washing and filtering. Table 1 shows the analysis of the main components of the corn stalks pretreated by polyethoxy ether.

[0029] (3) Weigh the corn stalks pretreated with the above alkali dosage of 10%, 20%, 30%, 40% and polyethylene glycol diglycidyl ether of 10% with the absolute dry weight of cellulose of 1.00g respectively. In two 250mL hydrolysis bottles, add 2.5mL of 1mol / L citric acid buffer, cellulase with enzyme dosage of 20FPU / g cellulose and appropriate amo...

Embodiment 2

[0037] A method for improving the enzymatic hydrolysis yield of alkaline pretreated wood fiber raw materials, the steps are as follows:

[0038] 1) Weigh 100g of absolutely dry corn stalks, use 10% (g / g) sodium hydroxide, at a solid-to-liquid ratio of 1:10 (g / mL), and the amount of polyethylene glycol diglycidyl ether is 5%, 10%, 20%, 30% (g / g), pretreated at 70°C for 3h.

[0039] 2) After the pretreatment, the materials are collected by washing and filtering. Table 2 shows the analysis of the main components of the corn stalk pretreated by polyethoxy ether.

[0040] (3) Weigh the above-mentioned alkali dosage of 1.00g with the absolute dry weight of cellulose as 10%, and the amount of polyethylene glycol diglycidyl ether as 5%, 10%, 20%, 30% pretreated corn stalks. In two 250mL hydrolysis bottles, add 2.5mL of 1mol / L citric acid buffer, cellulase with enzyme dosage of 20FPU / g cellulose and appropriate amount of distilled water to each hydrolysis bottle to make the total volume of ...

Embodiment 3

[0046] A method for improving the enzymatic hydrolysis yield of alkaline pretreated lignocellulosic raw materials, cellulase adsorption test, the specific steps are as follows:

[0047] 1) Weigh 100g of absolutely dry corn stalks, use 10% alkali (g / g), solid-liquid ratio 1:10 (g / mL), and 10% polyethylene glycol diglycidyl ether (g / g) Pretreated at 70°C for 3h, filtered and collected the pretreatment liquid and pretreatment materials.

[0048] 2) The pre-treated materials are collected by washing and filtering, and used for cellulose enzymatic hydrolysis.

[0049] 3) Weigh the corn stalks pretreated with 10% alkali and 10% polyethylene glycol diglycidyl ether with the absolute dry weight of cellulose as 1.00g into 2 250mL hydrolysis bottles. Add 2.5mL of 1mol / L citric acid buffer solution, cellulase with enzyme dosage of 20FPU / g cellulose and appropriate amount of distilled water to the hydrolysis bottle to make the total volume of water in the enzymatic hydrolysis system 50mL, and m...

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Abstract

The invention discloses a method for increasing the alkaline pretreated wood fiber raw material enzymolysis yield. The method comprises the steps of raw material pretreatment and enzyme hydrolysis, wherein in the step of pretreatment, polyethylene glycol diglycidyl ether is added to co-treat a substrate. The method for increasing the alkaline pretreated wood fiber raw material enzymolysis yield has the beneficial effects that during low-temperature alkaline pretreatment, polyethylene glycol diglycidyl ether is added to co-treat the substrate, thus effectively weakening nonspecific adsorption of lignin toward cellulase in the enzyme hydrolysis process and being beneficial to promoting cellulase hydrolysis; test results show that compared with existing research results, the enzyme hydrolysis yield is increased by 39.75% and reaches 71.26%; meanwhile, the method is simple in pretreatment process conditions, mild in reaction conditions and high in carbohydrate recovery rate, has the advantages of low energy consumption, medicine recovery, and the like, further has actual operation value and has good practicability.

Description

Technical field [0001] The invention relates to a process for preparing fermentable sugars, in particular to a method for improving the enzymatic hydrolysis yield of alkaline pretreated wood fiber raw materials. Background technique [0002] With the increasing demand for fossil resources and overexploitation of human society, the fossil resources on the earth are inevitably facing the fate of exhaustion; and the greenhouse effect caused by the use of a large number of fossil resources and the smog pollution that has erupted in recent years Evolution has become another major challenge facing mankind; at the same time, the "white pollution" caused by the large-scale use of non-degradable plastic products produced from petroleum resources has become a major public hazard in society, and there is an urgent need to find new degradable materials. Therefore, the adjustment of my country's energy structure brooks no delay. The use of renewable biomass resources to produce bioenergy, bio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/12C12P19/02
Inventor 赖晨欢勇强唐硕陈牧李鑫徐勇欧阳嘉余世袁
Owner NANJING FORESTRY UNIV
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