Method for efficiently saccharifying bagasse

A sugarcane bagasse and high-efficiency technology, applied in the field of high-efficiency saccharification of sugarcane bagasse, can solve the problems of difficulty in collection and transportation, low bulk density of lignocellulosic fibers, unfavorable large-scale industrial production, etc., and achieves improved raw material yield, small equipment investment, and reduced degradation. effect of loss

Active Publication Date: 2013-02-27
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the small bulk density of wood fiber, which only weighs about 100kg per cubic meter, it is difficult to collect and transport, which is not conducive to large-scale industrial production. At the same time, the ash content in bagasse is low, only about 2%, and the content of cellulose and hemicellulose is 5-10% higher than that of other lignocelluloses. In the same treatment method, the total sugar produced by enzymatic hydrolysis of 1 ton of bagasse can reach 550kg, while the total sugar per ton of other lignocellulose is only below 450kg

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In an appropriate reaction kettle, add 400 grams of chopped air-dried bagasse, then add sodium hydroxide and sodium sulfite mixed solution in a solid-to-liquid ratio of 1:8 (weight ratio), and the concentration of sodium hydroxide lye is 1.6%. , the concentration of sodium sulfite is 1.2%. After sealing the mouth, heat it at 95°C for 1.5 hours for pretreatment. The obtained slurry is centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor is concentrated to make sulfonated lignin products Gained solid phase adds in the container, adds water and regulates concentration to 20%, regulates pH to 4.8 with acid, regulates temperature to 50 ℃, adds cellulase in feed liquid, and enzyme amount is 30FPIU / gram substrate (in dry basis meter), the stirring speed is 100rpm / min, the enzymolysis time is controlled at 72 hours, and samples are taken every 24 hours. The DNS method is used to measure the total sugar concentration in the enzymolysis solution, and the high-perf...

Embodiment 2

[0033] In an appropriate reaction kettle, add 400 grams of chopped air-dried bagasse, then add sodium hydroxide and sodium sulfite mixed solution in a solid-to-liquid ratio of 1:8 (weight ratio), and the concentration of sodium hydroxide lye is 1.6%. , the concentration of sodium sulfite is 0.8%, after sealing the mouth, heat preservation at 90°C for 1.5 hours for pretreatment, the obtained slurry is centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor is concentrated to obtain a sulfonated lignin product; Gained solid phase adds in the container, adds water and regulates concentration to 20%, regulates pH to 4.8 with acid, regulates temperature to 50 ℃, adds cellulase in feed liquid, and enzyme amount is 30FPIU / gram substrate (in dry basis) ), the stirring speed is 100rpm / min, the enzymolysis time is controlled at 72 hours, sampling once every 24 hours, the total sugar concentration in the enzymolysis solution is measured by DNS method, and the glucose and woo...

Embodiment 3

[0035] In an appropriate reaction kettle, add 400 grams of chopped air-dried bagasse, then add sodium hydroxide and sodium sulfite mixed solution in a solid-to-liquid ratio of 1:8 (weight ratio), and the concentration of sodium hydroxide lye is 1.6%. , the concentration of sodium sulfite is 1.5%. After sealing the mouth, it is incubated at 95°C for 1.5 hours for pretreatment, and the obtained slurry is centrifuged in a 2000rpm centrifuge, and the centrifuged black liquor is concentrated to obtain a sulfonated lignin product; Gained solid phase adds in the container, adds water and regulates concentration to 20%, regulates pH to 4.8 with acid, regulates temperature to 50 ℃, adds cellulase in feed liquid, and enzyme amount is 30FPIU / gram substrate (in dry basis) ), the stirring speed is 100rpm / min, the enzymolysis time is controlled at 72 hours, sampling once every 24 hours, the total sugar concentration in the enzymolysis solution is measured by DNS method, and the glucose and w...

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Abstract

The invention provides a method for efficiently saccharifying bagasse, which comprises the following steps: mixing the bagasse with 0.1-20wt% catalyst solution in a weight ratio of 1:1-20 at the temperature of 70-140 DEG C, wherein the treatment time is 10-100 minutes, and the catalyst solution is mixed solution of sodium hydroxide and sodium sulfite; and then conducting solid-liquid separation, and adding cellulose to solids for enzymolysis. In the method, substances harmful to the enzymolysis and fermentation are not generated, so that the degradation loss of carbohydrates in biomass can be reduced, the enzymolysis yield of raw materials can be improved, and the production cost can be effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for efficiently saccharifying bagasse. Background technique [0002] Petroleum resources, which are the backbone of the world economy, are expected to be exhausted in a few decades. Therefore, the development and research of petroleum substitutes is imminent. At present, many countries are studying the preparation of fuel ethanol by bioconversion method using woody biological resources as raw materials to replace or partially replace petroleum with limited reserves. [0003] Woody biological resources include corn straw, wheat straw, sugar cane, etc., and their main components are cellulose, hemicellulose and lignin. Among them, cellulose and hemicellulose are the source of fermentable sugar, and the content accounts for 66-75% (the absolute dry weight of the fibrous raw material). It is a very mature process to produce ethanol from hexose through the fermen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
Inventor 邓远德尚海涛黄之文
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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