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Method for producing sodium gluconate by fermenting aspergillus niger

A fermentation technology of sodium gluconate and Aspergillus niger, which is applied in the field of bioengineering, can solve the problems of affecting the output and quality of sodium gluconate, yellowing, and smelling, and achieve the effects of shortening fermentation time, increasing yield and low cost

Inactive Publication Date: 2018-04-06
山东福洋生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the current status of reaction fermentation in this field, the reaction time is generally more than 20 hours, the ratio of sodium gluconate content in the original fermentation liquid is less than 32%, and the fermentation liquid is prone to be caused by improper control and excessive secondary metabolites during the fermentation process. And turn yellow, smelly, thereby greatly influenced the output and the quality of sodium gluconate

Method used

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  • Method for producing sodium gluconate by fermenting aspergillus niger
  • Method for producing sodium gluconate by fermenting aspergillus niger
  • Method for producing sodium gluconate by fermenting aspergillus niger

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Take a piece of Aspergillus niger, scrape the upper spores, insert it into the culture medium, cultivate it at 30°C until the spores are covered, and prepare a bacterial suspension for later use; the culture medium is a modified Martin agar medium;

[0030] Add 2.0kg of pure glucose, 2.2g of bran, 2.0g of potassium dihydrogen phosphate, 2.0g of magnesium sulfate, 6.2g of diammonium hydrogen phosphate, and 2.6ml of antifoaming agent to a 15L culture tank, make up to 10L, and sterilize at 115°C 20 minutes, then drop to 36°C, add 250ml bacterial suspension, control the temperature of the seed solution to 30°C; keep the pH of the seed solution at 5.2; control the dissolved oxygen (DO) ≥ 30%, and cultivate for 20 hours.

[0031]Add 5.4kg of pure glucose, 2.13g of potassium dihydrogen phosphate, 1.43g of magnesium sulfate, 1.11g of urea, 0.032g of diammonium hydrogen phosphate, and 7.02ml of defoamer to a 50L fermenter (first-level fermenter), and set the volume to 27L , ster...

Embodiment 2

[0039] Take a piece of Aspergillus niger, scrape the upper spores, insert it into the culture medium, cultivate it at 30°C until the spores are covered, and prepare a bacterial suspension for later use; the culture medium is a modified Martin agar medium;

[0040] Add 1.85kg of pure glucose, 2.1g of bran, 1.9g of potassium dihydrogen phosphate, 1.9g of magnesium sulfate, 6g of diammonium hydrogen phosphate, and 2.6ml of defoamer into a 15L culture tank, make up to 10L, and sterilize at 115°C for 20 minutes, then lowered to 36°C, added 250ml of bacterial suspension, controlled the temperature of the seed solution to 30°C; kept the pH of the seed solution at 5.2, controlled dissolved oxygen (DO) ≥ 30%, and cultivated for 20 hours.

[0041] The second step, fermentation: add 5kg of pure glucose, 2.11g of potassium dihydrogen phosphate, 1.40g of magnesium sulfate, 1.08g of urea, 0.28g of diammonium hydrogen phosphate, and 7.02ml of defoamer to the 50L fermenter (first-level ferment...

Embodiment 3

[0050] Take a piece of Aspergillus niger, scrape the upper spores, insert it into the culture medium, cultivate it at 30°C until the spores are covered, and prepare a bacterial suspension for later use; the culture medium is a modified Martin agar medium;

[0051] Add 2.15kg of pure glucose, 2.4g of bran, 2.2g of potassium dihydrogen phosphate, 2.2g of magnesium sulfate, 6.4g of diammonium hydrogen phosphate, and 2.6ml of defoamer into a 15L culture tank, make up to 10L, and sterilize at 115°C 20 minutes, then drop to 36°C, add 250ml of bacterial suspension, control the temperature of the seed solution to 30°C; keep the pH of the seed solution at 5.2, control dissolved oxygen (DO) ≥ 30%, and cultivate for 20 hours.

[0052] The second step, fermentation: add 5.8kg of pure glucose, 2.06g of potassium dihydrogen phosphate, 1.46g of magnesium sulfate, 1.13g of urea, 0.38g of diammonium hydrogen phosphate, and 7.02ml of defoamer to the 50L fermenter (first-level fermenter). , dilu...

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Abstract

The invention relates to a method for producing sodium gluconate by fermenting aspergillus niger. The method is characterized by comprising the steps that an aspergillus niger suspension is prepared;first nutrient salt is added into a seed tank with glucose as the raw material to prepare a seed culture solution, and the seed culture solution is inoculated into the aspergillus niger suspension; second nutrient salt is added into the seed tank with glucose as the raw material to prepare fermentation broth, the fermentation broth is inoculated into the seed solution for fermentation, in the fermentation process, mycelia are detected, the ventilation amount and the rotation speed are adjusted so that the length of aspergillus niger mycelia can be controlled in the range of 10-15 pm, and fermentation is completed when the glucose content in a fermentation tank is lower than 3 g / L; the fermentation broth is subjected to filtering, decoloring, crystallization, separation, drying and the liketo obtain a sodium gluconate finished product. Compared with an existing technology for producing sodium gluconate by fermenting aspergillus niger, the phenomena that the tank becomes yellow and smelly cannot be caused, the fermentation time can be shortened, and the yield and quality of sodium gluconate are improved.

Description

technical field [0001] The invention relates to the field of bioengineering, and more particularly, to a method for producing sodium gluconate by fermentation of Aspergillus niger. Background technique [0002] Sodium gluconate is an organic acid salt with good stability, non-toxicity and non-corrosiveness. It is widely used in medicine, food, chemical industry, light industry, etc. Sodium can increase the plasticity and strength of concrete; it can be used in medicine to adjust the acid-base balance in the human body and restore the nerves to normal; it can be used as a food additive in the food industry. Sodium Gluconate is chemically pure, non-corrosive, and of constant quality, characteristics that ensure reliable and reproducible results in applications. [0003] At present, the production methods of sodium gluconate mainly include electrolysis method, catalytic oxidation method, double enzyme method, microbial fermentation method and other methods. Among them, the el...

Claims

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Application Information

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IPC IPC(8): C12P7/58C12N1/14C12R1/685
CPCC12N1/14C12P7/58
Inventor 赵伟魏伯阳方建曹大鹏于哲
Owner 山东福洋生物科技股份有限公司
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