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High-hesperidinase-yield aspergillus niger KH005 and application thereof

A technology of hesperidinase and Aspergillus niger, applied in the field of microorganisms, can solve the problems of low efficiency of hesperidinase, poor thermal stability of hesperidinase and the like

Active Publication Date: 2016-06-15
成都康辉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a high-yield hesperidinase Aspergillus niger KH005, to solve the problems of low efficiency of existing bacterial strains producing hesperidinase, poor thermal stability of the produced hesperidinase, and reduce the production of hesperidinase cost to achieve industrial production

Method used

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  • High-hesperidinase-yield aspergillus niger KH005 and application thereof
  • High-hesperidinase-yield aspergillus niger KH005 and application thereof
  • High-hesperidinase-yield aspergillus niger KH005 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Aspergillus niger KH005 is used to produce hesperidinase:

[0062] Inoculate Aspergillus niger KH005 into the fermentation medium, ferment at 30°C and 150rpm for 6 days, then filter, remove the bacterial residue, collect and obtain the crude enzyme liquid, and store it in cold storage at 4°C for future use; the composition of the medium is as follows: : 2.0gNaNO 3 , 0.05gKCl, 1.0gK 2 HPO 4 , 0.5gMgSO 4 .7H 2 O, 0.01gFeSO 4 .7H 2 O, 10.0g hesperidin, 12.0g bran, 1000mL distilled water.

[0063] The enzyme activity of hesperidinase in the crude enzyme liquid measured is: 2349U.

[0064] Detection of the thermal stability of hesperidinase in the crude enzyme solution: cultivate the produced hesperidinase at 50°C for 72 hours, and measure the enzyme activity as 1358U; cultivate the produced hesperidinase at 55°C for 72 hours The measured enzyme activity was 1267U; the produced hesperidinase was cultured at 60°C for 24 hours, and the measured enzyme activity was 1189...

Embodiment 2

[0066] Aspergillus niger KH005 is used to produce hesperidinase:

[0067] Inoculate Aspergillus niger KH005 into the fermentation medium, ferment for 8 days at 35°C, 200rpm, then filter, remove the bacterial residue, collect and obtain the crude enzyme liquid, and store it in cold storage at 4°C for future use; the composition of the medium is as follows: : 2.0gNaNO 3 , 0.05gKCl, 1.0gK 2 HPO 4 , 0.5gMgSO 4 .7H 2 O, 0.01gFeSO 4 .7H 2 O, 10.0g hesperidin, 12.0g bran, 1000mL distilled water.

[0068] The enzyme activity of hesperidinase in the crude enzyme liquid measured is: 2548U.

[0069] Detection of the thermal stability of hesperidinase in the crude enzyme solution: cultivate the produced hesperidinase at 50°C for 72 hours, and measure the enzyme activity as 1459U; cultivate the produced hesperidinase at 55°C for 72 hours , The measured enzyme activity was 1320U; the produced hesperidinase was cultured at 60°C for 24 hours, and the measured enzyme activity was 1278U...

Embodiment 3

[0071] Aspergillus niger KH005 is used to produce hesperidinase:

[0072] Inoculate Aspergillus niger KH005 into the fermentation medium, ferment for 7 days at 33°C, 180rpm, then filter, remove the bacterial residue, collect the crude enzyme solution, and store it in a refrigerator at 4°C for future use; the composition of the medium is as follows: : 2.0gNaNO 3 , 0.05gKCl, 1.0gK 2 HPO 4 , 0.5gMgSO 4 .7H 2 O, 0.01gFeSO 4 .7H 2 O, 10.0g hesperidin, 12.0g bran, 1000mL distilled water.

[0073] The enzyme activity of hesperidinase in the crude enzyme liquid measured is: 2218U.

[0074] Detection of the thermal stability of hesperidinase in the crude enzyme solution: cultivate the produced hesperidinase at 50°C for 72 hours, and measure the enzyme activity as 1105U; cultivate the produced hesperidinase at 55°C for 72 hours , the measured enzyme activity was 985U; the produced hesperidinase was cultured at 60°C for 24 hours, and the measured enzyme activity was 1150U; the pr...

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Abstract

The invention discloses a high-hesperidinase-yield aspergillus niger KH005 and application thereof. The aspergillus niger KH005 with a preservation name being KH005 and a preservation number being CGMCC12101 is preserved in the China General Microbiological Culture Collection Center. The aspergillus niger is used for production of hesperidinase, degradation of hesperidin and production of hesperetin. The aspergillus niger KH005 is high in hesperidinase production efficiency, the activity is up to 2548U, valuable strain resources are provided for hesperidinase production, the produced hesperidinase can be used for degradation of hesperidin and production of hesperetin, and high conversion efficiency, mild and environment-friendly conditions and suitableness for industrial production are realized. In addition, the hesperidinase produced by the aspergillus niger KH005 has the advantages of high heat resistance, high enzyme activity and easiness in extraction and storage, thereby providing a new choice for production of high-purity commercial enzyme preparations and having high application and popularization values.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an Aspergillus niger KH005 with high hesperidinase production and application thereof. Background technique [0002] Hesperidinase is the key enzyme for hydrolyzing hesperidin and has high application value. Hesperetin monoglycoside, hesperetin, rhamnose and glucose can be obtained by hydrolyzing hesperidin with hesperidinase. In addition, hesperidinase is added to canned orange petals to hydrolyze the hesperidin in it, which can prevent white precipitation in canned oranges. [0003] Hesperidinase can be produced by a variety of microorganisms, such as Penicillium, Aspergillus oryzae, Aspergillus niger and Bacteroides. At present, domestic research on hesperidase-producing strains is still relatively small, and most of the reported hesperidase-producing strains are molds. [0004] At present, the research on hesperidinase in China basically stays in the laboratory stage and cann...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/24C12P17/06C12R1/685
CPCC12N9/2402C12P17/06C12N1/145C12R2001/685
Inventor 魏勇陈文清冯唯成
Owner 成都康辉生物科技有限公司
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