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Aspergillus niger An-19 strain and its use and fermentation method for the production of lovastatin

A technology of lovastatin and fermentation method, which is applied in the field of biotechnology microorganisms and can solve problems such as the lack of excellent industrial strains of lovastatin

Active Publication Date: 2019-04-16
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In response to the increasing demand for Lovastatin in the market, the lack of excellent industrial strains for producing Lovastatin, etc.

Method used

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  • Aspergillus niger An-19 strain and its use and fermentation method for the production of lovastatin
  • Aspergillus niger An-19 strain and its use and fermentation method for the production of lovastatin
  • Aspergillus niger An-19 strain and its use and fermentation method for the production of lovastatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Aspergillus niger ( Aspergillus niger ) Screening and identification of An-19 strain

[0034] 1 medium

[0035] ① PDA medium: 20% potato, 2% glucose, 2.0% agar powder, 1 L water, pH 5.5~6.0. For the isolation, purification and identification of Aspergillus strains.

[0036] ② PD medium: 20% potato, 2% glucose, 0.5% beef extract, 1 L water, pH 5.5~6.0. For the screening of Aspergillus strains with high Lovastatin production.

[0037] experimental method

[0038] 2.1 Isolation and purification of Aspergillus strains

[0039] Pick a small amount of hyphae from the surface of natural fermentation samples collected in different habitats and insert them on the surface of the PDA medium plate, culture at 26°C for 24 hours, and after the white fluffy mycelium grows, take a small amount of mycelium and transfer it to another PDA medium After continuing to culture on the plate for 3 days to produce spores, the typical characteristics of Aspergillus were observe...

Embodiment 2

[0052] The growth curve of embodiment 2 An-19 bacterial strain in 5 L fermentors, the generation and the preparation of Lovastatin

[0053] 1 strain: An-19 Aspergillus strain.

[0054] culture medium

[0055] ① PDA medium: the formula is the same as in Example 1.

[0056] ② PD culture medium: the formula is the same as in Example 1.

[0057] ③ Primary seed medium: 20% potato, 2% glucose, 0.5% beef extract, KH 2 PO 4 0.2%, MgSO 4 ·7H 2 O 0.1%, pH 5.5~6.0.

[0058] ④ Secondary seed medium: 10% lactose, 1% glycerol, 0.5% peptone, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O0.1%, NaNO 3 0.1%, pH 5.5~6.0.

[0059] ⑤ Fermentation medium: 18% lactose, 2% glycerin, 1% peptone, 0.5% yeast extract, MgSO 4 ·7H 2 O 0.1%, NaNO 3 0.2%, KH 2 PO 4 0.1%, ZnSO 4 0.2%, pH 5.5.

[0060] experimental method

[0061] 3.1 Cultivation of An-19 strain

[0062] A small amount of An-19 strain mycelium was picked with an inoculation needle, transferred to a PDA medium plate, and activated fo...

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Abstract

The invention belongs to the field of biotechnology microorganisms, and relates to a strain of Aspergillus niger ( Aspergillus niger ) An-19 strain, application and fermentation production method. Aspergillus niger ( Aspergillus niger ) An-19 strain, the preservation number is: CCTCC M 2015673, the preservation place is: China Center for Type Culture Collection, and the preservation date is: November 11, 2015. The An-19 bacterial strain involved in the present invention is screened from naturally fermented rice, and through morphological characteristics and 18S rDNA gene sequence analysis, it is identified as Aspergillus niger ( Aspergillus niger ). After optimization of the medium formula and culture conditions, the production of Lovastatin reached 267.02 mg / L after 116 hours of fermentation in a 5 L fermenter.

Description

technical field [0001] The invention belongs to the field of biotechnology microorganisms, and relates to a strain of Aspergillus niger ( Aspergillus niger ) An-19 strain, application and fermentation production method. Background technique [0002] Aspergillus niger ( Aspergillus niger ) is a filamentous fungus that widely exists in nature, belonging to Deuteromycota, Hyphomycetes, Moniliales, and Moniliaceae. Widely distributed in grains, plant products and soils all over the world, the optimum pH for growth is 3~7. It is an important industrial fermentation strain that can produce amylase, acid protease, cellulase, pectinase, glucose oxidase, citric acid, gluconic acid and gallic acid, etc. It is also one of the GRAS (Generally Regarded As Safe) strains identified by the US Food and Drug Administration (FDA), and is widely used in organic acids and industrial enzyme preparations. In addition, some strains can also produce physiologically active substances with importa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/685
CPCC12P17/06C12N1/145C12R2001/685
Inventor 蒋冬花任浩章婷韩肖飞郑婕施
Owner ZHEJIANG NORMAL UNIVERSITY
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