39 results about "Aspergillus sydowi" patented technology

The invention relates to a culture method of a fungus capable of poisoning plant parasitic nematodes, and a preparation method of a metabolite thereof and application thereof, and belongs to the technical field of microbial pesticides. A fungus strain (Aspergillus niger Y-61) related in the invention was collected in China General Microbiological Culture Collection Center on August 7, 2008, and the collection number is CGMCC No.2631. The taxonomic status of the Y-61 is a mitosporic fungus, hyphomycetes, moniliales, moniliaceae, aspergillus and aspergillus niger. The fungus metabolite is prepared by liquid fermentation and culture, has outstanding characteristics of high toxicity to the plant parasitic nematodes, especially root-knot nematodes, and has obvious nematodes killing effect; and the influence of the strain fermentation broth on Meloidogyne incognita juveniles (J2) and oocyst hatching is determined indoors. Treatment of the fermentation broth with different diluted concentrations is significantly different from the aseptic water treatment, and the preventive effect of the fermentation broth with less than one-fifth concentration is be equivalent to that of 10mug/ml of cadusafos. The fungus has good application and development prospects.

The invention relates to a cultivation of epiphyte that has plant infestation wireworm poisoning function and the metabolite manufacture method and the application. The strain of the epiphyte is Asipergillus. niger snf0407009 and the conserving code is CGMCC No.1714. It is made up from normal liquid fermenting and cultivating, and its metabolite has high poisoning function to plant infestation wireworm, especially root wireworm. The influence to the Meloidogyne incognita has been measured indoor. Different thickness fermenting liquid would have obviously influence to Meloidogyne incognita. One time fermenting liquid has the similar function as 10ug/mL aldicarb. It has good application prospect.

This invention provides the capture antibody and the detection antibody to detect the aspergillus, the two antibody is the single clone antibody of the cross oncocyte CCTCC NO.C200731 and CCTCC NO.C200730, they can unites the glucoprotein whose molecular weight is 25-100kDa in the cell wall of the aspergillus, the reaction with the Penicillium marneffei, white oidiomycosis, smoothness oidiomycosis, tropic oidiomycosis, close smoothness oidiomycosis, Candida krusei are all negative. The antibody in this invention can make into the immunity reagent to detect the aspergillus in common use.

The process of producing beta-mannase with Aspergillus niger belongs to the field of enzyme engineering technology. In the process of producing beta-mannase, corn cob, bran and other crop leftovers are used as the base material; and proper amount of konjaku powder, corn milk, inorganic salt, water, etc are added for solid fermentation with Aspergillus niger WA9024 strain. The present invention can obtain beta-mannase product possessing enzyme activity of 18912-22185 IU/g in test production or 12678-15921 IU/g in industrial production. The present invention provides also the recipe of fermenting culture medium and the fermenting conditions.

The invention relates to aspergillus tubingensis and a theabrownin producing method by liquid state fermentation thereof, and belongs to the technical field of biology. The production strain is aspergillus tubingensis KUtea 01, and is conserved in the China General Microbiological Culture Collection Center the address of which is number 3, courtyard 1, Beichenxi Road, Chaoyang District of Beijing. The conserving time is 30 December, 2014. The accession number is CGMCC No.10022. By inoculating the aspergillus tubingensis KUtea 01 into pasteurized green tea soup and culturing under culture conditions in a shake-flask single-strain manner for 3-4 days, the theabrownin content is 11-12 g/L, and the volumetric productivity is 11 times higher than that of solid fermentation. The aspergillus tubingensis and the method are advantageous in that production conditions and environments can be controlled easily and are not limited to the tea leaf production season, and automation, a high efficiency and a low cost can be achieved. The strain is directly bred in the green tea soup, does not need extra addition of carbon sources or nitrogen sources, and can rapidly and biologically convert polyphenols in the green tea soup into the macro-molecule water soluble theabrownin.

The invention provides a mouldy bran culture medium for enlarged culture of aspergillus niger and a culture method oriented to the problems that in the prior art, the number of bottles is too large due to the fact that the filling thickness of aspergillus niger strain mouldy bran cannot be too large, and strain contamination is caused by a small sampling rate. A carrier with corncob particles as a culture medium is adopted, a nutrient solution needed by growth of aspergillus niger is added, and the special culture medium is prepared; in addition, a culture mode and a bran preparing method are both innovated, and thus the defects in the prior art are well overcome. The detection rate of mouldy bran reaches 100%, seed tank contamination caused by the mouldy bran issue is excluded, and operation personnel are reduced by 80% through enlarging of each independent culture unit and simplification of the operation technological process, so that the personnel cost of enterprisers is lowered, and professional technological personnel can be easily trained; meanwhile, due to reduction of operation personnel, fluctuation of the quality of mouldy bran can be easily reduced, production scheduling is more flexible, and the output regulation of enterprisers can be quickly fed back in time.

The invention discloses a method for evaluating the activity of aspergillus niger mouldy bran spore. The method comprises the following steps: (1) washing cultured aspergillus niger mouldy bran spore with a phosphate buffer liquid, and filtering, thereby obtaining aspergillus niger mouldy bran spore suspension; (2) adding 0.055% (v/v) of Tween 80, and performing ultrasonic treatment, thereby obtaining uniformly dispersed monospore suspension, and adjusting the concentration of the monospore suspension to be 10<6>-10<7> pieces/mL; (3) preparing a 8-14mg/L methylene blue solution from the phosphate buffer liquid; (4) uniformly mixing the solutions of the steps (2) and (3) according to a ratio (v/v) of 1:1, sealing to react for 420 seconds, and scanning an absorbancy-time curve at the wavelength of 663nm; (5) testing the primary absorbancy and the absorbancy after the reaction is completed, of a reaction system, and representing the activity of cells according to the change of the absorbancy. By adopting the method, the culture time of mature seeds can be effectively shortened, the raw material utilization rate can be increased, and the technique is also applicable to evaluation on the activity of other microorganism spore.

The invention relates to epigenetic inheritance modification, and in particular relates to an aspergillus niger NY-1 as well as an improvement technique for improving the insecticidal activity of the aspergillus niger NY-1 and application of the aspergillus niger NY-1. The aspergillus niger NY-1 was collected in China Center for Type Culture Collection (CCTCC) on April 28th, 2014 with the collection number CCTCC NO: M2014169. The chemical epigenetic inheritance modification technique is used for improving the insecticidal activity of an NY-1 fermentation product and is applied to a microbial pesticide. The strain is separated from burdock collected in Anqiu, Shandong; an ITS zone is amplified through PCR, and the sequencing result shows that the similarity of the strain is 100% compared with a strain Aspergillus niger (Accession number: KF358715.1) in a Genebank database. An insecticidal silent gene of the aspergillus niger NY-1 is activated by adopting a chemical epigenetic modifier, so that the insecticidal activity of the NY-1 is improved, and the NY-1 is applied to the microbial pesticide. Compared with gene control and other silent gene activation technologies, the method has the advantages of quickness, low cost, obvious effect and the like.

The invention relates to Aspergillus niger pellets which are used for preventing and treating konjac southern blight and soft rot. Aspergillus niger xj is separated by fungal resource institute of Guizhou university, is collected in CCTCC (China Center for Type Culture Collection) (address: Wuhan University, Wuhan, China) at present and has the collection number CCTCCNO of M206021. The Aspergillus niger pellets contain Aspergillus niger fermented concentrate crude extract powder, microcrystalline cellulose (MCC), lactose and hydroxypropyl methylcellulose and can be used for preventing and treating konjac southern blight and soft rot.

The invention provides a screening method of aspergillus versicolor HY12. The screening method comprises the following steps: treating larvae of infected prodenia litura; preliminarily screening strains of prodenia litura; conducting a polypide inoculation experiment; screening out high pathogenic strains of the prodenia litura and performing molecular biological identification. According to the screening method provided by the invention, the defects that natural separation of pathogenic strains are low in efficiency and pertinence, are uncontrollable and are not repeated easily by adopting the traditional soil sample method are overcome; naturally dead larvae of the infected prodenia litura are collected for a pest area from the prodenia litura, the pathogenic strains are separated from rotten viscera in bodies of the larvae, and the prodenia litura can be infected by a leavening of the obtained aspergillus versicolor HY12, so that the prodenia litura can enter a pupal stage in advance, the leavening has higher teratogenesis rate and fatality rate on the prodenia litura in a pupation stage, a foundation is laid for later safe and effective prevention of the prodenia litura, and anovel template is provided for creation of a novel insecticidal inoculant.

The invention belongs to the technical field of biological control and particularly relates to bacillus sp. Mangrove-2008, an inoculant and an application and a method of the bacillus sp. Mangrove-2008 for inhibiting aspergillus niger growth. A collection number of the bacillus sp. Mangrove-2008 is CCTCC (China Center for Type Culture Collection) M2019355, and the bacillus sp. Mangrove-2008 is collected on May 14, 2019. The bacillus sp. Mangrove-2008 is shorter in growth cycle; required fermentation conditions are relatively simple; the bacillus sp. Mangrove-2008 is used for antagonizing the aspergillus niger growth; potential adverse effects of chemical control on environment and grain safety are avoided; the bacillus sp. Mangrove-2008 and a metabolic product of the bacillus sp. Mangrove-2008 can effectively inhibit the aspergillus niger growth; and the bacillus sp. Mangrove-2008 can be processed and developed as a dosage form of the living inoculant and the metabolic product and hasa very important application value.

The invention relates to the technical field of microorganisms and biology, in particular to aspergillus sydowii as well as a separation method and application thereof. The aspergillus sydowii JDQMZZ-1 has been deposited in China General Microbiological Culture Collection Center on March 12, 2020 with a deposit number of CGMCC No.19278, and has nitrogen fixation and strong secretion ability; has a strong inhibitory effect on fungi and bacteria, can also promote germination of plant seeds, and can achieve growth-promoting effects by adjusting a content of plant endogenous hormones; can promote growth of underground part of seedlings of Paris polyphylla after applying to the seedlings of Paris polyphylla, but does not activate plant autoimmunity, and effectively avoids a result of activating plant autoimmunity leading to reduced biomass. Meanwhile, the aspergillus sydowii JDQMZ-1 increases chlorophyll content of plants and reduces malondialdehyde content, which indicates that when the aspergillus sydowii JDQMZ1 acts on seedlings of Paris polyphylla, an influence of the environment on the seedlings of Paris polyphylla is reduced, damage to cell membranes is reduced, and abiotic stress resistance of the seedlings of Paris polyphylla is improved.

The invention discloses Aspergillus sydowii MNP-2 and application thereof in synthesis of dibenzo [b, e] oxa compounds, the Aspergillus sydowii MNP-2 can produce the dibenzo [b, e] oxa compounds at high yield, and the strain is easy to culture and convenient for amplification fermentation, and has potential of industrial and large-scale production. A high-yield dibenzo [b, e] oxa compound (I) is separated from an aspergillus sydowii MNP-2 fermentation product, the yield is 4.3 mg/g, and the separation process is simple. The dibenzo [b, e] oxa compound (I) has anti-tumor activity, and can be subjected to structural modification in the later stage to prepare a derivative of the compound to greatly improve the activity of the compound, so that anti-tumor drugs are prepared.

An Asperillus niger 5450 strain (CGMCCNO.0496) is disclosed, which is obtained by screening from soil and well grows in culture medium and culture liquid. After growing in culture liquid for 2-3 days, it has very high reaction activity and contains epoxy hydrolase with corresponding selectivity. It can be directly used in asymmetric hydrolysis of recemic 1,2-epoxide to obtain S-type epoxide and R-type 1.2-diol.

The invention provides a strain of aspergillus versicolor HY12. The bacterial colonyof the aspergillus variabilis HY12 is round, the texture is dense and soft, hyphae arein white cotton fiber shapes,a large number of conidiacan be produced, are brown powder and have concentric annulation upheavals. The strain of the aspergillus variabilis HY12has a high growth and reproduction speed in a short time, can produce a large number of conidia, can infect prodenialitura and has higherfatality rate and teratogenicity to the prodenialitura, an experimental foundation is laid for the prevention and control of prodenialitura, and a favorable reference is provided for the prevention and control of other pests in the field.

The invention relates to the technical field of biology, and in particular, relates to glucose-resistant beta-glucosidase as well as an expression gene and application thereof. Aspergillus sydowii L1 capable of producing beta-glucosidase when glucose is used as a substrate is screened from mangrove forest soil, genes bgAs1 with similar expression quantities when glucose, microcrystalline cellulose and cellobiose are used as unique carbon sources for induction are screened by a transcriptome comparison method, in-vitro expression is carried out by a pichia pastoris expression system, and the recombinant beta-glucosidase BgAs1 is obtained after purification. The beta-glucosidase has relatively high glucose tolerance, salt tolerance and wide substrate specificity, shows the potential of replacing a commercial enzyme Nov188 when being cooperated with cellulase to hydrolyze BgAs1 on bagasse, and has a good industrial application prospect.

The invention relates to a method for breeding microbes by using the protoplast fusion technology, in particular to a method for breeding endo-inulinase strains by using the protoplast fusion technology, and belongs to the technical field of heredity. According to the method, two aspergillus nigers are used as starting strains and subjected to activation by a culture medium containing inulin as well as shake culture by burdock juice, cellulase and helicase are utilized to hydrolyze cell walls of aspergillus niger hypha cells, and the oscillation is performed at constant temperature to prepare protoplasts; through the fusion of the protoplasts, the enzyme activities of endo-inulinase are respectively improved by 35.08 percent and 48.26 percent compared with the two aspergillus nigers. The protoplasts can be regenerated after the fusion, after ten generations passage experiments, the deviation of the enzyme activity of the endo-inulinase is smaller compared with regenerated strains, and inheritable characters are stable; the strains can be used for industrial production of endo-inulinase.