A kind of indole cyanoalkaloid compound and its preparation and application

A technology of alkaloids and indole cyano, which is applied in the field of antibacterial agents, can solve problems such as water and soil pollution, biological and human health hazards, and achieve the effect of inhibiting Proteus mirabilis

Active Publication Date: 2019-07-26
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In recent years, with the development of aquaculture scale and the extensive use of traditional antibiotics in the aquaculture process, multi-drug resistance of pathogenic bacteria in aquaculture animals and bacteria in the aquaculture environment has become increasingly common, and the extensive use of chemically synthesized drugs has negative effects on water bodies and soils. It has brought serious pollution, which has caused direct and potential harm to organisms and human health.

Method used

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  • A kind of indole cyanoalkaloid compound and its preparation and application
  • A kind of indole cyanoalkaloid compound and its preparation and application
  • A kind of indole cyanoalkaloid compound and its preparation and application

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The structure of the indole cyanoalkaloid compound derived from the endophytic fungus of seaweed is shown in formula (I).

[0025]

Embodiment 2

[0027] The preparation method of the alkaloid compound as shown in formula (I):

[0028] Get well-grown Aspergillus versicolor (Aspergillus versicolor) DL-29 bacterial classification on the plate and inoculate in potato dextrose liquid (PDB) medium, put 300 milliliters of medium in every 1 liter of Erlenmeyer flasks, altogether 50 bottles, room temperature static fermentation 30 days, filter and collect the mycelium and fermentation broth separately.

[0029] The PDB liquid medium consists of 200 milliliters of potato juice, 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract, 500 milliliters of aged sea water and 300 milliliters of distilled water per liter.

[0030] Aspergillus versicolor (Aspergillus versicolor) DL-29 strain was preserved in China Center for Type Culture Collection CCTCC on November 25, 2011, address: Wuhan University, China, preservation number is CCTCC M 2011421, classification is named Aspergillus versicolor, strain number for DL-29.

[0...

Embodiment 3

[0038] The difference from Example 2 is that

[0039]Get the well-grown fungus Aspergillus versicolor (Aspergillus versicolor) DL-29 bacterial classification on the flat plate and inoculate in Jerusalem artichoke glucose liquid medium, put 300 milliliters of culture medium in every 1 liter of Erlenmeyer flasks, totally 50 bottles, static fermentation at room temperature for 20 days, Filter and collect mycelium and fermentation broth separately.

[0040] The Jerusalem artichoke glucose liquid medium consists of 500 milliliters of Jerusalem artichoke tuber juice per liter, 10 grams of glucose, 2.0 grams of sodium nitrate, and 500 milliliters of aged seawater.

[0041] Collect about 15 liters of fermentation broth, extract three times with ethyl acetate, and concentrate under reduced pressure; the mycelium is dried and pulverized, extracted three times with ethyl acetate, and concentrated under reduced pressure; the concentrate is detected by thin layer chromatography (TLC) (petr...

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Abstract

The invention relates to the field of bacteriostats, and concretely relates to a seaweed endophytic fungus-derived natural indocyano alkaloid compound, a preparation method thereof, and an applicationthereof in bacteriostasis. The specific structural formula of the compound is represented by formula (I). The preparation method comprises the following steps: inoculating a fungal culture medium with the seaweed endophytic fungus Aspergillus versicolor DL-29, carrying out fermentation culturing, and separating and purifying the obtained fermentation product to obtain the indocyano alkaloid compound of the formula (I). Bacteriostatic activity experiments of the alkaloid compound show that the diameter of the inhibition zone of the compound at 30 [mu]g / tablet is 7 mm.

Description

technical field [0001] The invention relates to the field of antibacterial agents, in particular to a natural indocyanine alkaloid compound derived from seaweed endophytic fungi, a preparation method thereof and an application in antibacterial aspect. Background technique [0002] The aquaculture industry occupies an important proportion in the national economy. At present, there are various types of aquaculture, but almost all of them are threatened by diseases. The frequent occurrence of aquaculture diseases not only causes significant economic losses, but also lays hidden dangers for the quality and safety of aquatic products. According to the monitoring results of aquaculture animal diseases, there are more than 200 kinds of aquaculture diseases in recent years, of which bacterial diseases account for more than 48.0%. Among bacterial aquatic animal diseases, vibriosis caused by Vibrio bacteria is the most important bacterial disease. The disease has a wide range of occu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D498/22C12P17/18A01N43/90A61P1/00A61P31/04C12R1/66
CPCA01N43/90C07D498/22C12P17/188
Inventor 季乃云刘向红
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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