Aspergillus sydowii and application thereof in promoting plant growth and preventing and treating plant diseases

A technology of Aspergillus polypolyps and plants, which can be used in plant growth regulators, plant growth regulators, pest control, etc., and can solve the problem of poor cold resistance, drought resistance and strong light radiation, which restricts the development of the artificial planting industry of heavy buildings , Artificial planting is difficult and other problems, to achieve strong inhibitory effect, increase plant chlorophyll content, improve the effect of abiotic stress resistance

Active Publication Date: 2021-04-20
QINGHAI NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To sum up, slow growth, serious diseases, and poor stress resistance are the main reasons restricting the development of the artificial planting industry of P.
Narrow leaf Chonglou is one of the main varieties of Huachonglou. It is mainly distributed in Qinba mountainous areas such as Shaanxi and Gansu. Due to slow growth, sensitivity to chemical fertilizers, severe rot, and poor stress resistance, especially cold resistance, drought resistance, Due to poor resistance to strong light radiation and other reasons, artificial planting is difficult, the market demand is large, and wild resources are severely damaged

Method used

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  • Aspergillus sydowii and application thereof in promoting plant growth and preventing and treating plant diseases
  • Aspergillus sydowii and application thereof in promoting plant growth and preventing and treating plant diseases
  • Aspergillus sydowii and application thereof in promoting plant growth and preventing and treating plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment one, strain isolation

[0037] (1) Rinse the harvested Huazhonglou seeds with running water for 10 minutes, blot the surface moisture with filter paper, soak in 75% ethanol for 30 seconds, rinse with sterile water 2 to 3 times, and then disinfect the surface with 0.2% mercury chloride for 10 minutes , washed with sterile water for 3 to 4 times, and 100 μL of sterile water from the final rinse was applied to the tiger bengal solid medium as a control.

[0038] (2) Cut the surface-sterilized Huazhonglou seeds into small pieces with a sterilized blade, place them in a mortar sterilized by high-temperature dry heat at 200°C, add 10 mL of sterile saline, and grind them thoroughly.

[0039] (3) Take 100 μL of the above liquid, repeat 3 times, spread it on a solid tiger bengal plate, and culture it in the dark at 28°C, and different fungi will grow for about 10 days.

[0040] (4) Pick a little hyphae and repeatedly purify them for 4 to 6 generations on a PDA plate,...

Embodiment 2

[0042] Embodiment two, bacterial strain identification

[0043] Inoculate the above strains into liquid PDB medium, culture at 28°C and 200rpm for 5 to 7 days, collect the bacteria by centrifugation at 4°C and 8000rpm, and after the tissue disruptor is fully broken, use the SDS method to extract genomic DNA. PCR amplification. Primers are:

[0044] ITS1: 5'-TCCGTAGGTGAACCTGCGG-3'

[0045] ITS4: 5'-TCCTCCGCTTATTGATATGC-3'

[0046] The PCR reaction system is 50μl: Buffer (Mg2+plus) 10μl, forward and reverse primers 2μl each, dNTP Mixture (each 2.5mM) 4μl, HS DNA Polymerase (2.5Μ / μl) 0.5μl, DNA template 1μl, ddH2O 31.5μl.

[0047] PCR amplification conditions: denaturation at 98°C for 10 sec, annealing at 56°C for 15 sec, extension at 72°C for 1 min and 30 sec, 30 cycles, and extension at 72°C for 5 min.

[0048] The amplified products were sent to Beijing Qingke Xinye Biotechnology Co., Ltd. for sequencing. The sequencing results were compared in the NCBI database blast...

Embodiment 3

[0052] Embodiment 3, qualitative detection of nitrogen fixation and phosphorus solubilization activity of Aspergillus polypolyphaga JDQMZZ-1

[0053] Qualitative detection of nitrogen fixation activity: Prepare Ashubei medium, add 0.5% Congo red 5ml / L, sterilize and make a plate for use, pick a small amount of mycelia from the edge of the preserved Aspergillus polydoctoris JDQMZZ-1 colony and inoculate on the above Cultivate in the Ashubei culture medium at 28°C for 7 days in the dark, and observe the growth of the colonies.

[0054] Congo red is a brown-red powder, which is yellow-red when dissolved in water, and orange when dissolved in alcohol. It is used as an acid-base indicator with a color change range of 3.5 to 5.2. , shown as a red circle. The size of the circle and the depth of the color can reflect the strength of nitrogen fixation ability to a certain extent.

[0055] Qualitative detection of phosphorus-solubilizing ability: inoculate Aspergillus polypolyphagum J...

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PUM

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Abstract

The invention relates to the technical field of microorganisms and biology, in particular to aspergillus sydowii as well as a separation method and application thereof. The aspergillus sydowii JDQMZZ-1 has been deposited in China General Microbiological Culture Collection Center on March 12, 2020 with a deposit number of CGMCC No.19278, and has nitrogen fixation and strong secretion ability; has a strong inhibitory effect on fungi and bacteria, can also promote germination of plant seeds, and can achieve growth-promoting effects by adjusting a content of plant endogenous hormones; can promote growth of underground part of seedlings of Paris polyphylla after applying to the seedlings of Paris polyphylla, but does not activate plant autoimmunity, and effectively avoids a result of activating plant autoimmunity leading to reduced biomass. Meanwhile, the aspergillus sydowii JDQMZ-1 increases chlorophyll content of plants and reduces malondialdehyde content, which indicates that when the aspergillus sydowii JDQMZ1 acts on seedlings of Paris polyphylla, an influence of the environment on the seedlings of Paris polyphylla is reduced, damage to cell membranes is reduced, and abiotic stress resistance of the seedlings of Paris polyphylla is improved.

Description

technical field [0001] The invention relates to the field of microorganisms and biotechnology, in particular to a strain of Aspergillus polypolyphaga and its application in promoting plant growth and preventing and controlling plant diseases. Background technique [0002] Aspergillus sydowii (Aspergillus sydowii) belongs to Ascomycota, Deuteromycetes, Hyphomycetes, Stemsaceae, Aspergillus, Nedulans, Versicolor group. At present, the research on Aspergillus polydoctoris mainly focuses on the absorption of cadmium and lead ions in the soil by the strain, the main components of the secondary metabolites of the strain, and the degradation of pesticides and organic substances. The patent (CN201810252492.1) discloses a The method of remediating cadmium-contaminated soil by using Aspergillus polypolyphaga combined with Indian mustard has the advantages of economical efficiency, no secondary pollution, and beautifying the landscape. Wang Shuai et al. isolated Aspergillus polydoctor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/34A01P1/00A01P3/00A01P21/00C12R1/66
Inventor 杨涛祁宏山王治业马永贵杨晖谢惠春马玉兰赵志敏赵疆方彦昊
Owner QINGHAI NORMAL UNIV
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