Aspergillus niger fungus capable of poisoning plant parasitic nematodes, preparation method and application thereof
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A technology for killing nematodes and fungi, which is applied in the field of fungi for poisoning and killing plant parasitic nematodes and their preparation
Inactive Publication Date: 2009-06-03
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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The deposit date is August 7, 2008
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Embodiment 1
[0017] Embodiment 1: the cultivation of bacterial strain A.niger Y-61
[0018] The mycelium of A. niger Y-61 was inoculated on the test tube agar medium, and the medium formula was PDA medium, that is, potato 200g; glucose 20g; agar 17g; distilled water 1000mL. Incubate at 25°C for 7 days to obtain test tube species.
[0019] Then inoculate the test tube into a 500mL Erlenmeyer flask (125mL per bottle) liquid medium, and the medium formula is (percentage by weight): maltose (2.4%), glucose (0.8%), peptone (0.06%), yeast extract (0.06%) %), KH 2 PO 4 ·7H 2 O (0.14%), MgSO 4 (0.04%), Cacl 2 2H 2 O 0.15%, FeCl 3 0.098%, ZnSO 4 ·7H 2 O 0.0002%, the rest is distilled water, pH 6.5. At 25°C to 28°C, the shaker speed is 220r·min -1 ~240r·min -1 , fermentation 168h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.
Embodiment 2
[0020] Embodiment 2: the cultivation of bacterial strain A.niger Y-61
[0021] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:
[0022] The liquid medium formula is: maltose (1.6%), glucose (0.6%), peptone (0.08%), yeast extract (0.12%), KH 2 PO 4 ·7H 2 O (0.10%), MgSO 4 (0.04%), Cacl 2 2H 2 O 0.15%, FeCl 3 0.098%, ZnSO 4 ·7H 2 O 0.0002%, pH 7.
Embodiment 3
[0023] Embodiment 3: the cultivation of bacterial strain A.niger Y-61
[0024] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:
[0025] Liquid medium formula is: maltose (2.2%), glucose (0.8%), peptone (0.12%), yeast extract (0.14%), KH 2 PO 4 ·7H 2 O (0.10%), MgSO 4 (0.07%), Cacl 2 2H 2 O 0.15%, FeCl 3 0.098%, ZnSO 4 ·7H 2 O 0.0002%, pH 6.
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Abstract
The invention relates to a culture method of a fungus capable of poisoning plant parasitic nematodes, and a preparation method of a metabolite thereof and application thereof, and belongs to the technical field of microbial pesticides. A fungus strain (Aspergillus niger Y-61) related in the invention was collected in China General Microbiological Culture Collection Center on August 7, 2008, and the collection number is CGMCC No.2631. The taxonomic status of the Y-61 is a mitosporic fungus, hyphomycetes, moniliales, moniliaceae, aspergillus and aspergillus niger. The fungus metabolite is prepared by liquid fermentation and culture, has outstanding characteristics of high toxicity to the plant parasitic nematodes, especially root-knot nematodes, and has obvious nematodes killing effect; and the influence of the strain fermentation broth on Meloidogyne incognita juveniles (J2) and oocyst hatching is determined indoors. Treatment of the fermentation broth with different diluted concentrations is significantly different from the aseptic water treatment, and the preventive effect of the fermentation broth with less than one-fifth concentration is be equivalent to that of 10mug/ml of cadusafos. The fungus has good application and development prospects.
Description
Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus for poisoning and killing plant parasitic nematodes, a preparation method and application thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual ...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/685
Inventor 刘霆刘伟成刘建华裘季燕卢彩鸽刘德文
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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