Screening method of aspergillus versicolor HY12

A technology of Aspergillus versicolor and a screening method, which is applied in the field of screening of Aspergillus versicolor HY12, can solve the problems of low efficiency of pathogenic strains, low pertinence, and difficult to repeat, and achieves inhibition of the growth of various bacteria, high lethality and pathogenicity. Distortion rate, not easy to repeat the effect

Inactive Publication Date: 2019-04-09
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a screening method for Aspergillus versicolor HY12, which overcomes the defects of low efficiency, low pertinence, uncontrollable, and difficult repetition of the traditional method of using soil samples to naturally isolate pathogenic strains. Collect the larvae of susceptible Spodoptera litura that died naturally in moth-infested areas, and isolate the pathogenic strain from the rotting internal organs of the larvae. The obtained fermented product of Aspergillus versicolor HY12 can infect Spodoptera litura, making it enter the pupal stage in advance. It has a high lethality and teratogenicity rate against Spodoptera litura during pupation, lays the foundation for safe and effective control of Spodoptera litura in the later stage, and provides a novel template for the creation of new insecticides

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  • Screening method of aspergillus versicolor HY12
  • Screening method of aspergillus versicolor HY12
  • Screening method of aspergillus versicolor HY12

Examples

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Embodiment 1

[0033] Screening of Aspergillus versicolor HY12 strain

[0034] Collect the larvae of susceptible Spodoptera litura that died naturally from the infested area of ​​Spodoptera litura, screen the susceptible larvae of Spodoptera litura without mold layer on the surface, immerse them in 75% alcohol for 2 seconds, disinfect the body surface, and peel off under aseptic conditions The rotted viscera in the larvae were removed, and the rotted viscera were homogenized. The homogenate was diluted with sterile water into 10-fold, 100-fold, and 1000-fold gradients, and spread on the surface of a screening medium plate, and cultured at 25.6°C for 5 days. Wherein the screening medium is HECK medium, comprising the following components in parts by weight: 4 parts of glucose, 2 parts of peptone, 0.025 part of potassium permanganate, 0.5 part of cycloheximide, 0.2 part of gentamicin, 0.048 part of ampicillin , 2 parts of agar and 100 parts of water.

[0035] Pick a single colony in the HECK m...

Embodiment 2

[0053] Identification of Aspergillus versicolor HY12 strain

[0054] Inoculate the Aspergillus strain (HY12) obtained in Example 1 onto the SDAY medium, cultivate it in a constant temperature incubator at 25.6°C, observe its colony character and color, and pick mycelium from the colony to make a microscopic examination, observe The color and properties of the mycelia, conidiophores and sporulation axes; after the colony produced sporulation, the conidia were picked up for microscopic examination to observe the conidia’s properties, color, size, and photograph. The results were as follows: figure 1 shown. Depend on figure 1 It can be seen that after culturing on SDAY medium for 14 days, the diameter of the colony reaches 66.3mm, the colony is round, white cotton-like mycelia, the colony is thicker, and the texture is dense and soft; a large number of conidia are produced in the later stage, and the spores are brown powdery, and the powder layer Thick, with concentric ridges. ...

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Abstract

The invention provides a screening method of aspergillus versicolor HY12. The screening method comprises the following steps: treating larvae of infected prodenia litura; preliminarily screening strains of prodenia litura; conducting a polypide inoculation experiment; screening out high pathogenic strains of the prodenia litura and performing molecular biological identification. According to the screening method provided by the invention, the defects that natural separation of pathogenic strains are low in efficiency and pertinence, are uncontrollable and are not repeated easily by adopting the traditional soil sample method are overcome; naturally dead larvae of the infected prodenia litura are collected for a pest area from the prodenia litura, the pathogenic strains are separated from rotten viscera in bodies of the larvae, and the prodenia litura can be infected by a leavening of the obtained aspergillus versicolor HY12, so that the prodenia litura can enter a pupal stage in advance, the leavening has higher teratogenesis rate and fatality rate on the prodenia litura in a pupation stage, a foundation is laid for later safe and effective prevention of the prodenia litura, and anovel template is provided for creation of a novel insecticidal inoculant.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a screening method for Aspergillus versicolor HY12. Background technique [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in increasing agricultural production and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (namely, resistance to pesticides, residence of pesticide residues, and Rampant Resurgence) has also brought a series of ecological and environmental problems. The long-term and extensive use of chemical pesticides has led to an increase in pesticide-resistant pests, especially in the past 10 years, the resistance of cotton bollworms, aphids, diamondback moths, Spodoptera litura, and spider mites to pyrethroids and organophosphorus chemical pesticides has increased hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12N3/00C12R1/66
CPCC12N1/02C12N1/14C12N3/00
Inventor 谭琳胡秋龙刘双清柏连阳汤心砚程予奇
Owner HUNAN AGRICULTURAL UNIV
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