Erythromycin degrading bacterium W1 and application thereof

A technology of erythromycin and degrading bacteria, which is applied in the fields of fungi, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of limited bacterial resources and hindering erythromycin-contaminated microbial remediation technology, etc. Achieve the effect of high erythromycin degradation characteristics and avoid secondary pollution

Inactive Publication Date: 2021-12-07
CHANGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are few reports on the screening of erythromycin-degrading bacteria for the pollution caused by macrolide antibiotics such as erythromycin at home and abroad, and the reported strain resources are also very limited, which greatly hinders the development of erythromycin. The application of contaminated microbial remediation technology also limits the elucidation of erythromycin-degrading enzymes and degradation pathways

Method used

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  • Erythromycin degrading bacterium W1 and application thereof
  • Erythromycin degrading bacterium W1 and application thereof
  • Erythromycin degrading bacterium W1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Cultivate erythromycin-degrading bacteria W1 in YPD liquid medium, collect the bacteria by centrifugation, resuspend with sterile water, and inoculate the selected strain with 1% (1g of bacteria per 100mL inorganic salt medium) to a concentration of 100.0 mg / L erythromycin basic liquid inorganic salt medium, placed in a constant temperature shaker at 120r / min, 30 ℃ for 80h, erythromycin-degrading bacteria have obvious growth, and the degradation efficiency of erythromycin is relatively high.

Embodiment 2

[0068] Inoculate erythromycin-degrading bacteria W1 into 100 mL of sterile MSM at an inoculum amount of 1% (1 g of bacteria per 100 mL of inorganic salt medium), add erythromycin to a final concentration of 100 mg / L, and place at 30°C , statically cultivated for 80h, and the erythromycin degradation rate was determined to be 83.53% (see figure 2 ).

Embodiment 3

[0070] The erythromycin-degrading bacteria W1 was inoculated in 100mL of erythromycin-containing wastewater at an inoculum size of 1% (adding 1g thallus in every 100mL of inorganic salt medium). L, at 30°C, cultured statically for 80 hours, the degradation rate of erythromycin was determined: 68.52% (see Figure 3)

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Abstract

The invention discloses an erythromycin degrading bacterium W1 and application thereof, and belongs to the technical field of biology. The erythromycin degrading bacterium is Aspergillus sydowii of which the collection number is CGMCC NO.23059. Experiments prove that the erythromycin degrading bacterium W1 disclosed by the invention can survive in an environment taking erythromycin as a unique carbon source, and has a very high erythromycin degrading activity. The erythromycin degrading bacterium W1 provided by the invention can be applied to degradation of the erythromycin in a polluted environment, and has a good application value on the aspects of soil pollution treatment and environmental remediation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to an erythromycin-degrading bacterium W1 and its application in the field of erythromycin degradation in soil and water. Background technique [0002] Erythromycin belongs to macrolide antibiotics, has strong antibacterial effect, and is widely used. The mechanism of action is mainly combined with the 50S subunit of ribonucleosomes, inhibiting peptide acyl transferase, and affecting the migration of ribonucleosomes. It hinders the growth of peptide chains, inhibits the synthesis of bacterial proteins, and is a bacteriostatic agent. And erythromycin is a kind of multi-component antibiotics, and erythromycin A is mainly used clinically, and its chemical structure consists of erythronolide (erythronolide), deoxyaminoethyl sugar (desosamine) and erythromycin sugar ( cladnose) consists of 3 parts. Erythromycin has a stable structure and is difficult to remove through natural de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C02F3/34C12R1/66C02F101/34
CPCB09C1/10C02F3/34C02F2101/34
Inventor 任建军王珍珠张晋赵建张健于长勇李春雨
Owner CHANGZHOU UNIV
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