Erythrocin degrading bacterium W7 and application thereof

A technology of erythromycin and degrading bacteria, applied in the biological field, to achieve the effect of avoiding secondary pollution and high erythromycin degradation characteristics

Inactive Publication Date: 2021-12-07
CHANGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing treatment processes, such as biological aerated filters, can only reduce trace amounts of erythromycin in wastewater. To more effectively degrade erythromycin residues in the environment, specific microorganisms must be relied on

Method used

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  • Erythrocin degrading bacterium W7 and application thereof
  • Erythrocin degrading bacterium W7 and application thereof
  • Erythrocin degrading bacterium W7 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Adjust the pH of the inorganic salt medium to 6.0, 7.0, and 8.0 with dipotassium hydrogen phosphate and potassium dihydrogen phosphate, and inoculate the erythromycin-degrading bacteria W7 at 1% (add 1 g of bacteria per 100 mL of inorganic salt medium) Inoculate in 75.0mL sterilized inorganic salt medium, the concentration of erythromycin in the medium is 100mg / L, and then put it into a shaking table to avoid light and shake it for culture, the shaking speed is 180r / min, and the culture temperature is 35°C. After 3 days, 1 mL of sample was taken, filtered through a 0.22 μm nylon filter membrane, and the content of erythromycin was determined by HPLC. After determination, when the pH was 7.0, the degradation rate of erythromycin was the highest, which was 36.9% (see figure 2 ).

Embodiment 2

[0071] Inoculate the degraded bacterial strain obtained by enrichment into 75mL inorganic salt medium, the inoculum amount of the degradative bacterial strain is 1% (1g thalline is added in every 100mL inorganic salt medium), and the concentration of erythromycin in the medium is respectively 50mg / L, 100mg / L, 200mg / L, 300mg / L, and then placed in a shaking table to avoid light for shaking culture, the shaking speed was 180r / min, and the culture temperature was 35°C. After 3 days of culture, 1mL was sampled, filtered through a 0.22μm nylon filter membrane and used HPLC measures the content of erythromycin, after measuring, when erythromycin concentration is 50mg / L, erythromycin degradation rate is the highest, is 58.5% (see image 3 ).

Embodiment 3

[0073] Inoculate the enriched degradation strain into 75mL inorganic salt medium, the inoculum size is 1%, the concentration of erythromycin in the medium is 100mg / L, and then put it into a shaker to avoid light for shaking culture, and the shaking rate is 180r / L min, the culture temperature was set at 20°C, 25°C, 30°C, 35°C and 40°C, 1 mL was sampled after 3 days of culture, filtered through a 0.22 μm nylon filter membrane, and the content of erythromycin was determined by HPLC. After determination, the temperature was 35 ℃, the highest degradation rate is 28% (see Figure 4 ).

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Abstract

The invention discloses an erythrocin degrading bacterium W7 and application thereof, and belongs to the technical field of biology, the erythrocin degrading bacterium is paracoccus versutus, and the preservation number of the erythrocin degrading bacterium is CGMCC (China General Microbiological Culture Collection Center) NO.22980. Experiments prove that the erythromycin degrading bacterium W7 disclosed by the invention can survive in an environment taking erythromycin as a unique carbon source, and has very high erythromycin degrading activity. The erythromycin degrading bacterium W7 provided by the invention can be applied to the degradation of erythromycin in a polluted environment, and has a good application value in the aspects of soil pollution treatment and environmental remediation.

Description

technical field [0001] The invention belongs to the field of biological technology, and specifically relates to an erythromycin-degrading bacterium W7 and its application in the field of erythromycin degradation in soil and water. Background technique [0002] Erythromycin is a multi-component antibiotic. Erythromycin A is mainly used clinically. Its chemical structure is composed of three parts: erythromycin lactone with a 14-membered ring, deoxyaminoethyl sugar, and erythromycin sugar. Erythromycin has obvious inhibitory effects on both Gram-positive and Gram-negative bacteria, especially Mycoplasma, Chlamydia, Legionella, Spirochetes and Rickettsia, because of its low toxicity, and other macrocyclic Compared with lactone antibiotics, they have the characteristics of similar or stronger antibacterial activity, and become the first widely used clinical drugs in this kind of compounds. Although mycin has good pharmacological effects, long-term metabolism will accumulate a c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/34C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C02F2101/34
Inventor 牛东泽张乔攀张晋朱周娟于长勇张健呼和涛力王美阳靳丰宁
Owner CHANGZHOU UNIV
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