Mouldy bran culture medium for enlarged culture of aspergillus niger and culture method

A technology for expanding cultivation and cultivation methods, which is applied to the bran koji culture medium and the field of cultivation of Aspergillus niger species bran koji, which can solve the problems of occasional bacterial contamination, large number of bottles, and difficulty in scheduling, so as to reduce the quantity , Increase the loading effect

Active Publication Date: 2016-06-22
JIANGSU GUOXIN UNION ENERGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is that the sampling rate of bran koji is low, and the number of cultured bottles is large. When testing bran koji, only about 5% of bran koji can be extracted for shake-flask fermentation testing.
Since each bottle of bran koji is an independent culture unit, about 150 bottles of bran koji need to be inserted into each seed tank, so even if the random inspection is qualified, the quality of the bran koji cannot be fully guarante

Method used

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  • Mouldy bran culture medium for enlarged culture of aspergillus niger and culture method

Examples

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Effect test

Embodiment 1

[0038] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 800g of corn steep liquor powder and glucose, 120g of ammonium sulfate, mix and dissolve with water, and make up to 6L after dissolution. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0039]Aspergillus niger CICC40021 preserved in China Industrial Microbiology Culture Collection Center (CICC) was used as the strain source. Put 1.9kg of the finished culture medium into a 20L culture container, add 3.2L of water, sterilize at 121°C for 90min, and inoculate after cooling. The culture conditions are as follows: the ventilation rate is 1:0.25 volume: (volume min) in the first three days, the ventilatio...

Embodiment 2

[0043] The selection of corn cob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 200g each of corn steep liquor powder and glucose, 30g of ammonium sulfate, and 5L of soybean meal enzymatic hydrolysis filtrate, add water to mix and dissolve, and make up to volume after dissolving 6L. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0044] Put 1.9kg of the finished culture medium into a 20L culture container, add 3.2L of water, sterilize at 121°C for 90min, and inoculate after cooling. The culture conditions are as follows: the ventilation rate is 1:0.25 volume: (volume min) in the first three days, the ventilation rate is 1:0.5 volume: (volume min) in the next seven days, the fir...

Embodiment 3

[0048] The selection of corncob carrier and the preparation of soybean meal hydrolyzate are as mentioned above, and the preparation of nutrient solution is as follows: take 400g of corn steep liquor powder and glucose, 60g of ammonium sulfate, 2L of soybean meal enzymatic hydrolysis filtrate, add water to mix and dissolve, and make up to volume after dissolving 6L. Mix 6L of nutrient solution evenly into 8kg of corncobs, and after the corncobs have completely absorbed the nutrient solution, dry them in a low-temperature drying oven at a temperature not exceeding 60°C. After drying, it is the finished culture medium.

[0049] Put 3kg of the finished culture medium into a 30L culture container, add 4.8L of water, sterilize at 121°C for 90min, inoculate and culture after cooling. The culture conditions are as follows: the ventilation rate is 1:0.25 volume: (volume min) in the first three days, the ventilation rate is 1:0.5 volume: (volume min) in the next seven days, the first f...

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Abstract

The invention provides a mouldy bran culture medium for enlarged culture of aspergillus niger and a culture method oriented to the problems that in the prior art, the number of bottles is too large due to the fact that the filling thickness of aspergillus niger strain mouldy bran cannot be too large, and strain contamination is caused by a small sampling rate. A carrier with corncob particles as a culture medium is adopted, a nutrient solution needed by growth of aspergillus niger is added, and the special culture medium is prepared; in addition, a culture mode and a bran preparing method are both innovated, and thus the defects in the prior art are well overcome. The detection rate of mouldy bran reaches 100%, seed tank contamination caused by the mouldy bran issue is excluded, and operation personnel are reduced by 80% through enlarging of each independent culture unit and simplification of the operation technological process, so that the personnel cost of enterprisers is lowered, and professional technological personnel can be easily trained; meanwhile, due to reduction of operation personnel, fluctuation of the quality of mouldy bran can be easily reduced, production scheduling is more flexible, and the output regulation of enterprisers can be quickly fed back in time.

Description

technical field [0001] The invention relates to a microbial culture medium and a culture method, in particular to a bran koji culture medium and a culture method for expanding the cultivation of Aspergillus niger strain bran koji produced by citric acid fermentation. Background technique [0002] Citric acid is widely used as a sour agent in the food industry, known as the first edible sour agent, and is also the most important organic acid produced by fermentation; in addition, citric acid and citrates, esters and citric acid derivatives have Wide range of uses. [0003] The preparation of bran koji from Aspergillus niger is the basis of citric acid production, and the quality of bran koji is directly related to the quality of the product. Now the way of making koji in production enterprises is still the traditional way, that is, to use bran to cultivate in a glass triangular flask. The skin contains about 50% water, loosely put into a 2000ml Erlenmeyer bottle, about 35 g...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/685
CPCC12N1/14C12N2500/30
Inventor 胡志杰刘桂芳刘振强高金宝蒋小东李小晔彭艳红孙福新高超
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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