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Aspergillus niger and method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger

A technology for fructooligosaccharides and Aspergillus niger, which is applied in the field of oligosaccharides, can solve the problems of complex separation and purification process, limited mass transfer uniformity, and high cost of enzymatic conversion, achieves simple process, and reduces the cost of fermentation tanks and their auxiliary equipment. The effect of inputting and avoiding the inactivation of enzymes

Active Publication Date: 2014-09-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that there are many steps, the enzyme is very easy to be inactivated during the immobilization process, the conversion rate is low, and the uniformity of mass transfer is limited because sucrose and converted products have to pass through the immobilized particles.
In addition, the separation and purification process of the enzyme is complicated and the enzyme activity is partially lost during the process, unstable in the three-dimensional structure environment, easy to inactivate and other disadvantages, and the price of the enzyme is expensive, resulting in high cost of enzymatic conversion. These aspects are severely restricts its industrial application

Method used

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  • Aspergillus niger and method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger
  • Aspergillus niger and method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The first step: inoculate the spores of Aspergillus niger FOS-0620 into the seed liquid medium, and the inoculum size is 1×10 5 A / L, at 33 DEG C, the bacterial cell solution after 16 hours of cultivating on a shaker with a rotating speed of 150r / min is made into a seed liquid; the seed liquid culture medium is: 20g / L sucrose, 30g / L corn flour, 100g / L Yeast extract, 2g / L NaNO 3 ;Stir and dissolve evenly, then sterilize, and cool for later use;

[0036] The second step: Inoculate the seed solution obtained in the first step into the liquid medium containing the enzyme-producing inducer at a volume ratio of 5:100, and cultivate it at 33°C for 24 hours at a rotational speed of 150r / min. The whole cell solution of Aspergillus niger was made from the cell solution of Aspergillus niger; the liquid medium containing the enzyme inducer was: 70g / L sucrose, 20g / L yeast extract, 15g / L corn flour, 3g / L NaNO 3 ;Stir and dissolve evenly, then sterilize, and cool for later use;

[0...

Embodiment 2

[0042] The first step: inoculate the spores of Aspergillus niger FOS-0620 into the seed liquid medium, and the inoculum size is 1×10 10 A / L, at 28 ℃, under the condition of rotating speed of 100r / min, the bacterial liquid after culturing for 24h is the seed liquid; the seed liquid culture medium is: 50g / L sucrose, 7g / L malt powder, 10g / L peptone, 3g / L NaCl; stir and dissolve evenly, sterilize with sterilizer, cool for later use;

[0043] Step 2: Inoculate the seed solution obtained in the first step into a liquid medium containing an enzyme-producing inducer at a ratio of 10:100 by volume, and cultivate it for 16 hours at 28°C with a rotation speed of 100r / min. The thalline solution of Aspergillus niger whole cell thalline solution is made; The liquid medium containing the enzyme inducer is: 70g / L sucrose, 35g / L yeast extract, 15g / L corn flour, 12g / L K 2 HPO 4 , 1.5g / LMgSO 4 .7H 2 O, 10g / L NaNO 3 ;Stir and dissolve evenly, then sterilize, and cool for later use;

[0044]...

Embodiment 3

[0048] The first step is to inoculate the spores of Aspergillus niger FOS-0620 into the seed liquid medium, and the inoculum size is 1×10 6 cells / L, at 25°C, with a rotation speed of 120r / min, the bacterial cell solution was cultivated for 20h to make seed liquid; the seed liquid culture medium was: 30g / L sucrose, 5g / L malt powder, 7g / L Peptone, 50g / L yeast extract, 1.5g / L NaCl, 1.5g / L NaNO 3 ;Stir and dissolve evenly, then sterilize, and cool for later use;

[0049] The second step is to inoculate the seed solution obtained in the first step into the liquid medium containing the enzyme-producing inducer at a volume ratio of 8:100, and cultivate it for 20 hours at 25°C with a rotation speed of 300r / min. The thalline solution is made into Aspergillus niger whole cell thalline solution; The liquid medium containing the enzyme inducer is: 10g / L sucrose, 50g / L yeast extract, 20g / L corn flour, 6g / L K 2 HPO 4 , 0.5g / L MgSO 4 .7H 2 O, 6.5g / L NaNO 3 ;Stir and dissolve evenly, th...

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Abstract

The invention discloses aspergillus niger and a method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger. The aspergillus niger FOS-0620 is an aerobe, has black spores and milk-white hyphae, and is collected in China General Microbiological Culture Collection Centre (CGMCC for short) on September 28, 2012. The method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger comprises the following steps of: (1) preparing aspergillus niger whole-cells by virtue of the aspergillus niger FOS-0620 according to claim 1; and (2) catalytically producing fructo-oligosaccharide by virtue of the aspergillus niger whole-cells. The product obtained by the method disclosed by the invention is detected that the content (occupying total solids) of the fructo-oligosaccharide is not less than 50% via high-pressure liquid chromatography; and the method disclosed by the invention is simple in process, convenient to operate, high in enzymatic activity, high in conversion efficiency, and important in industrial value.

Description

technical field [0001] The invention belongs to the technical field of oligosaccharides, and relates to a fermentation production method of fructo-oligosaccharides, in particular to a method for producing fructo-oligosaccharides catalyzed by Aspergillus niger and its whole cells. Background technique [0002] Fructooligosaccharides, also known as Fructooligosaccharides (abbreviated as FOS), are composed of 1 to 3 fructosyls combined with sucrose through β-1,2 glycosides to generate 1-Kestose, Nystose and Nystose (1 F -Fructofuranosylnystose). FOS has excellent properties such as promoting the proliferation of probiotics in the intestines, such as bifidobacteria, detoxifying and cleaning the intestines, improving human immunity, improving lipid metabolism, preventing tooth decay, and not being absorbed and utilized by the digestive tract. It is now widely used in health care in food ingredients. [0003] At present, there are two methods for producing fructooligosaccharide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/18C12R1/685
Inventor 刘冬梅周康范梦珂叶嘉伦
Owner SOUTH CHINA UNIV OF TECH
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