35 results about "High pressure liquid chromatography procedure" patented technology

In one aspect the invention is a composition comprising a prepolymer which is the reaction product of di(isocyanatophenyl) methane or a polymeric di(isocyanatophenyl) methane having an isocyanato functionality of about 2.1 to about 3.0 with a mixture of one or more diols and one or more triols wherein the ratio of isocyanate equivalents to hydroxyl equivalents in the reaction mixtures used to prepare the prepolymer is from about 1.2 to about 1.8 and the ratio of diol to triol where the diisocyanate is a di(isocyanatophenyl) methane in the reaction mixture is from about 5:1 to about 1:1. and where the isocyanate is a polymeric di(isocyanatophenyl) methane is from about 8:1 to about 4:1; wherein the prepolymer could further react with an isocyanate reactive monofunctional compound. The prepolymer herein has an isocyanate content of about 0.5 to about 1.5 percent by weight, a free isocyanate monomer content of about 1.0 percent by weight or less as measured by high pressure liquid chromatography. These prepolymers can be used in many applications including adhesive, sound dampening sealer and coating and especially in making adhesives or adhesive systems with a low content of monomeric isocyanate to bond together similar or dissimilar substrates such as metal, glass, ceramics, plastic and painted steel panel.

A cannabis based therapeutic product for treatment of chronic pain produced by separating hash resin from plant material of the cannabis plant, pressing the hash resin to expel oil, leaving spent hash resin, extracting further cannabinoids from the spent hash resin using MCT oil, separating the cannabis compounds from the oils using high pressure liquid chromatography, flash chromatography, or similar techniques, and processing selected cannabis compound formulations into a pill, tablet, liquid, or other form suitable for administration as a therapeutic product for treatment of chronic pain, optionally with the further step of enriching the product with a formulation comprising at least 1% by weight purified -myrcene, prior to processing into a final product.

The present invention provides a polycarbonate resin produced by the continuous interfacial process characterized in that after alkaline hydrolysis with sodium hydroxide, the polycarbonate resin contains an amount of 0.01 to 150 ppm of carbamate compounds according to formula (1)said amount measured by high pressure liquid chromatography, wherein R1 and R2 independently of one another denote hydrogen or C1-C12-alkyl, or together denote C4-C12-alkylidene, and R3 and R4 independently of one another denote hydrogen. C1-C12-alkyl or phenyl, or together with the carbon atom to which they are bonded form cyclohexyl or trimethylcyclohexyl, the process comprising phosgene reacting with at least one bisphenol at 8 to 17% molar excess of phosgene relative to the bisphenol.

The invention is composition comprising red blood cells in an aqueous suspension medium and one or more cis di-ahls; wherein more than 6 percent by weight of the hemoglobin in the red blood cells is S-Alc glycated hemoglobin. In another embodiment, the invention is a method comprising contacting red blood cells in a suspension medium having a concentration of S-Alc glycated hemoglobin of greater than 6 percent by weight of the hemoglobin in the red blood cells with a sufficient amount of one or more cis di-ahls such that the concentration of S-Alc glycated hemoglobin in resulting composition as measured by high pressure liquid chromatography, immunoassay and boronate affinity methods is consistent.

The invention crushes the kernels or seeds of Luo Han Guo, extracts fat-soluble substances with an organic solvent, removes the organic solvent in the fat-soluble substances, and obtains the crude product of Luo Han Guo squalene. Then pass the crude product of Luo Han Guo squalene through a silica gel chromatography column, elute with an organic solvent, collect the colorless part of the eluate, and remove the organic solvent by vacuum distillation to obtain the fine product of Luo Han Guo squalene. The squalene content in the crude product is more than 40%, and the squalene content in the fine product is more than 95%. The signals are consistent with the squalene standard sample spectrum after determination by high-pressure liquid chromatography, infrared spectroscopy, and nuclear magnetic resonance. , and the product has the unique fragrance of squalene, which can completely replace squalene, which can only be extracted from the liver of deep-sea sharks. Luo Han Guo squalene boutique can be used as a squalene standard sample. The development and utilization of Luo Han Guo squalene has huge social and economic benefits.

A system and a method are provided for calculating one or more indicative properties, e.g., one or more of the cetane number, octane number, pour point, cloud point and aniline point of oil fractions, from the density and high pressure liquid chromatography (HPLC) data of a sample of the crude oil.

The invention discloses aspergillus niger and a method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger. The aspergillus niger FOS-0620 is an aerobe, has black spores and milk-white hyphae, and is collected in China General Microbiological Culture Collection Centre (CGMCC for short) on September 28, 2012. The method for catalytically producing fructo-oligosaccharide by virtue of whole-cells of aspergillus niger comprises the following steps of: (1) preparing aspergillus niger whole-cells by virtue of the aspergillus niger FOS-0620 according to claim 1; and (2) catalytically producing fructo-oligosaccharide by virtue of the aspergillus niger whole-cells. The product obtained by the method disclosed by the invention is detected that the content (occupying total solids) of the fructo-oligosaccharide is not less than 50% via high-pressure liquid chromatography; and the method disclosed by the invention is simple in process, convenient to operate, high in enzymatic activity, high in conversion efficiency, and important in industrial value.

The invention discloses a chromanone compound, and a preparation method and application thereof. The chromanone compound is Tobchromanone A and is separated from aromatic tobacco; the molecular formula is C14H16O4; and the structure is shown in the specification. The preparation method of the chromanone compound comprises the following steps: crushing an aromatic tobacco sample, performing ultrasonic extraction with 95% ethanol for 3-5 times, merging the extracting solutions, and concentrating under reduced pressure to obtain an extract; and performing primary separation on the extract through silica gel column chromatography, and then performing further separation through high pressure liquid chromatography to obtain the required compound, namely the Tobchromanone A. The compound has a better cytotoxic effect on human neuroblastoma cells (SHSY5Y) with the IC50 value up to 2.8 mu M, and indicates a medium cytotoxic effect on other measured cell strains.

Disclosed is a composition for textile softener containing a cationic surfactant, the ratio of which C7-C21 alkyl substituents analyzed by HLPC (high pressure liquid chromatography) or GC (gas chromatography) is 0.6 or more, as an effective ingredient, a sheet for textile softener including the same, and method of softening a textile using the sheet. Since the composition for textile softener has excellent dissolving and dispersing effect even in low temperature water, the sheet containing this composition is used at a rinse time during washing procedures to represent excellent anti-static effect and textile softening effect to textiles and clothes.

The invention discloses a method for catalytic production of fructo-oligosaccharides by aspergillus oryzae whole cells. The method comprises the following steps of 1, whole-cell catalyst preparation comprising inoculating a seed medium with aspergillus oryzae spores, preparing a seed solution under a certain condition, inoculating a liquid fermentation enzyme-production medium for producing an enzyme inducer with the seed solution, preparing a whole-cell aspergillus oryzae solution under a certain condition, collecting aspergillus oryzae, filtering the aspergillus oryzae by a filter cloth of 200 to 400 meshes, and carrying out washing, re-filtration and vacuum freeze drying to obtain aspergillus oryzae whole cells, and 2, aspergillus oryzae whole cell-based fructo-oligosaccharide catalytic production comprising carrying out conversion under a certain conditions and carrying out filtration and vacuum concentration of the obtained fructo-oligosaccharide liquid to obtain fructo-oligosaccharide syrup. A high pressure liquid chromatography test on the fructo-oligosaccharide product proves that the solids comprise greater than or equal to 50% of total oligosaccharides. The method has simple processes, is convenient for operation, and has high enzyme activity, high conversion efficiency and an important industrial value.

The invention discloses a fingerprint detection method of white ginseng fungus, which uses high-pressure liquid chromatography to measure the fingerprint with reference to a schizophyllin reference substance. The invention establishes the HPLC characteristic fingerprint of the white ginseng fungus, overcomes the singleness and one-sidedness of the existing quality standards, and can fully reflect the quality information of the white ginseng fungus, thereby achieving the purpose of more comprehensive and effective quality control of the white ginseng fungus. The white ginseng bacteria fingerprint spectrum obtained by the method of the invention has many peaks, good peak shape, easy identification, high similarity, accuracy and reliability.

The invention relates to a new technique for purifying and preparing eptifibatide. At present, the eptifibatide is separated, purified and produced by adopting opposite phase high pressure liquid chromatography. However, mass production is not easy to realize, and the equipment are expensive. Separation and purification are carried out by the method by applying two solvent systems which are not dissolved in each other and make epicyclic motion at high speed in a chromatographic column tube; the processing steps comprises: a. synthetic crude product of the eptifibatide is dissolved by solvent;b. the dissolved crude product of the eptifibatide is separated and purified by high-speed centrifugation separation chromatography (FCPC), and is tested by a uv detector from the distance of 230nm, so that target peak is collected by subsection; c. the collected cut fraction is tested by HPLC, wherein, the cut fraction with the purity higher than 98% is bended to be treated by the next step, andthe cut fraction with the purity lower than 98% is recycled and purified again; d. ion exchange is carried out on the cut fraction with qualified purity to remove trifluoroacetic acid (TFA), and thenthe cut fraction is transformed into acetic acid eptifibatide. The new technique has no irreversible adsorption and the advantages of no loss of sample, no pollution, high speed and high efficiency, and is suitable for mass production.