Plant squalene and its preparation method

A technology of squalene and Luo Han Guo, applied in the field of Luo Han Guo squalene and its preparation, can solve the problems of low reproduction rate, long growth period of deep-sea sharks, difficult sources, etc., and achieve huge social and economic benefits

Active Publication Date: 2006-11-15
桂林中族中药股份有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, people can only extract squalene from the liver of deep-sea sharks, and only trace amounts of seabuckthorn are found in plants.
However, deep-sea sharks have a long growth period, low reproductive rate, and difficult sources. Therefore, finding another source of squalene is a problem that people are eager to solve.

Method used

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  • Plant squalene and its preparation method
  • Plant squalene and its preparation method
  • Plant squalene and its preparation method

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preparation example Construction

[0013] Preparation method process step of the present invention is:

[0014] a. Broken the kernels or seeds of Luo Han Guo, and extracting fat-soluble substances with organic solvents;

[0015] b, remove the organic solvent in the fat-soluble substance, promptly make the crude product of Luo Han Guo squalene;

[0016] c. Pass the crude product of Luo Han Guo squalene through a silica gel chromatography column, elute with an organic solvent, and collect the colorless part of the eluate;

[0017] d. Remove the organic solvent from the collected colorless eluent by distillation under reduced pressure, so as to obtain the fine product of Luo Han Guo squalene.

Embodiment 1

[0019] Take 1000 grams of Luo Han Guo seed kernels and crush them, use 10 liters of petroleum ether to extract fat-soluble substances from them by percolation, and remove the petroleum ether to obtain 520 grams of Luo Han Guo squalene crude product, which contains 42.5% of squalene after testing;

Embodiment 2

[0021] Take 1000 grams of Luo Han Guo seeds and crush them, use 10 liters of petroleum ether to extract fat-soluble substances from them by percolation, and remove the petroleum ether to obtain 71 grams of Luo Han Guo squalene crude product, which contains 41.5% of squalene after testing;

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Abstract

The invention crushes the kernels or seeds of Luo Han Guo, extracts fat-soluble substances with an organic solvent, removes the organic solvent in the fat-soluble substances, and obtains the crude product of Luo Han Guo squalene. Then pass the crude product of Luo Han Guo squalene through a silica gel chromatography column, elute with an organic solvent, collect the colorless part of the eluate, and remove the organic solvent by vacuum distillation to obtain the fine product of Luo Han Guo squalene. The squalene content in the crude product is more than 40%, and the squalene content in the fine product is more than 95%. The signals are consistent with the squalene standard sample spectrum after determination by high-pressure liquid chromatography, infrared spectroscopy, and nuclear magnetic resonance. , and the product has the unique fragrance of squalene, which can completely replace squalene, which can only be extracted from the liver of deep-sea sharks. Luo Han Guo squalene boutique can be used as a squalene standard sample. The development and utilization of Luo Han Guo squalene has huge social and economic benefits.

Description

(1) Technical field: [0001] The invention relates to monk fruit squalene extracted from plant kernels or seeds and a preparation method thereof. (two) background technology: [0002] The chemical name of squalene is: 2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene. Squalene has the functions of enhancing physical fitness and anti-fatigue, and can be used for liver disease treatment and has certain anti-cancer, anti-cancer, moisturizing and beautifying effects. At present, people can only extract squalene from the liver of deep-sea sharks, and only trace amounts of seabuckthorn are found in plants. However, deep-sea sharks have a long growth period, low reproductive rate, and difficult sources. Therefore, finding another source of squalene is a problem that people are eager to solve. (3) Contents of the invention: [0003] The present invention can provide the public with a method for preparing grosvenor squalene extracted from the kernel or seeds of grosvenor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C7/10C07C11/21
Inventor 陈全斌义祥辉程忠泉
Owner 桂林中族中药股份有限公司
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