Method for extracting natural activated products from orange drops

Abstract

The invention belongs to the technical field of natural product extraction from oranges, and specifically relates to a method for continuously extracting natural activated products such as synephrine, hesperidin and limonin from physiological orange drops. The method comprises the following steps: using orange drops which are dried and crushed into 20-100 meshes as raw materials, adding 50-90% ethanol according to a weight / volume ratio of (1:10)-(1:50), extracting under reflux at 40-80 DEG C for 1-3 hours, filtering, recovering ethanol from the filtrate, and obtaining a concentrated solution for later use; adding a small number of chloroform crystals into the concentrated solution, adding water for layering, directly heating the water layer to 75 DEG C, filtering, adding 0.1mol / L hydrochloric acid to regulate the pH value to 3-4, and precipitating to obtain a hesperidin crude product; carrying out spin drying on the obtained chloroform layer solution, adding a right number of acetone crystals, and carrying out vacuum drying to obtain a synephrine crude product; and carrying out spin drying on the acetone solution after the crystal filtration, adding dichloromethane for dissolution, filtering, adding isopropanol into the solution according to a volume ratio of 1:2, and crystallizing to obtain a limonin crude product. Qualitative and quantitative analysis are carried out on the three resulting activated products through high-pressure liquid chromatography, and the result shows that the hesperidin has the highest purity (91.41%).

Description

technical field

[0001] The invention belongs to the technical field of plant natural product extraction, and in particular relates to a method for continuously extracting natural active products such as synephrine, hesperidin and limonin from citrus physiological fruit drop. Background technique

[0002] Citrus belongs to Rutaceae (Citrus) plant in taxonomy, and is the largest fruit in the world. In 2005, the world's citrus output reached more than 100 million tons, and China was about 15 million tons, second only to Brazil and China. United States (Ye Xinggan, Citrus Processing and Comprehensive Utilization. Beijing: China Light Industry Press, 2005, 12-15; Li Jianmin et al., Combined extraction of essential oil, pigment, pectin and hesperidin from citrus peel. Biomass Chemical Engineering, 2007a, 41(1):22-24). my country is rich in citrus germplasm resources and is the origin of many citrus varieties in the world. Many citrus varieties from all over the world were origina...

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