Plant squalene and its preparation method
A technology of squalene and plants, applied in the fields of extraction and purification/separation, organic chemistry, etc., can solve the problems of low reproduction rate, long growth period of deep-sea sharks, difficult sources, etc., and achieve huge social and economic benefits
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[0013] Preparation method process step of the present invention is:
[0014] a. Broken the kernels or seeds of Luo Han Guo, and extracting fat-soluble substances with organic solvents;
[0015] b, remove the organic solvent in the fat-soluble substance, promptly make the crude product of Luo Han Guo squalene;
[0016] c. Pass the crude product of Luo Han Guo squalene through a silica gel chromatography column, elute with an organic solvent, and collect the colorless part of the eluate;
[0017] d. Remove the organic solvent from the collected colorless eluent by distillation under reduced pressure, so as to obtain the fine product of Luo Han Guo squalene.
Embodiment 1
[0019] Take 1000 grams of Luo Han Guo seed kernels and crush them, use 10 liters of petroleum ether to extract fat-soluble substances from them by percolation, and remove the petroleum ether to obtain 520 grams of Luo Han Guo squalene crude product, which contains 42.5% of squalene after testing;
Embodiment 2
[0021] Take 1000 grams of Luo Han Guo seeds and crush them, use 10 liters of petroleum ether to extract fat-soluble substances from them by percolation, and remove the petroleum ether to obtain 71 grams of Luo Han Guo squalene crude product, which contains 41.5% of squalene after testing;
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