Method for preparing kestose and nystose through yacon

A technology of kestose and kestose, which is applied in the preparation of sugar derivatives, chemical instruments and methods, oligosaccharides, etc., can solve the problems of lack of mature technology and less research

Inactive Publication Date: 2012-04-11
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current research focuses on the preparation of high-purity fructooligosaccharides, but there are few studies on the preparation of single components such as kestose and kestose tetraose in fructooligosaccharides, and there is still a lack of mature technology

Method used

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  • Method for preparing kestose and nystose through yacon
  • Method for preparing kestose and nystose through yacon
  • Method for preparing kestose and nystose through yacon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0009] The preparation of oligosaccharides in implementation case 1 Yacon

[0010] Raw materials: Yacon fruit is produced in Yunnan, with a maturity of 95%, stored at 4°C for half a month. Slice the yacon, dry it at 50°C, and pulverize it with a high-speed pulverizer to make yacon powder.

[0011] Yacon powder → 80°C hot water extraction → centrifugation, suction filtration → retain the supernatant, and repeatedly extract the residue → combine the extracts → concentrate → 80% ethanol (v / v) precipitation → retain the supernatant → concentrate → freeze-dry → Yacon Oligosaccharides

Embodiment example 2

[0012] Qualitative and quantitative tests of oligosaccharides in implementation case 2 yacon

[0013] HPLC

[0014] Sample treatment: The yacon oligosaccharide sample was formulated into a 5 mg / ml solution with mobile phase, filtered through a 0.22 μm microporous membrane, degassed by ultrasonic, and 20 μl of the filtrate was taken for analysis.

[0015] Preparation of standard solution: prepare 5mg / ml standard solutions of fructose, glucose, sucrose, kestose, and kestosetetose reference substances respectively with mobile phase, pass through a 0.22μm filter membrane, and ultrasonically degas for later use. .

[0016] Chromatographic conditions:

[0017] Chromatographic column: APS-2HYPERSIL;

[0018] Column temperature: 30°C;

[0019] Mobile phase: acetonitrile-water (75:25, V / V), mix well, and ultrasonically degas before use;

[0020] Flow rate: 0.8ml / min;

[0021] Injection volume: 20 μl.

[0022] Detector: differential detector

[0023] According to the HPLC chrom...

Embodiment 3

[0024] Embodiment 3: Preparation of kestose and kestetose in fructooligosaccharides

[0025] After repeated thin-layer chromatography experiments, it was determined that the developing solvent glacial acetic acid: chloroform: absolute ethanol: water = 3: 11: 11: 1 was used as the eluent for silica gel column chromatography. Take an appropriate amount of silica gel, add eluent, mix well, and transfer to a glass chromatography column (1.6cm×50cm). Take 1g of oligosaccharide sample, put it on the column by dry method, and then use eluent to elute, and the elution speed is 1ml / min. The eluate was collected in a test tube, concentrated, and freeze-dried. Thin layer chromatography was then performed. The chromogen is 2.5g diphenylamine, add 5mL aniline, 25mL 85% phosphoric acid to mix and dissolve, add acetone to dilute to 250mL, refrigerate for later use (refrigeration can be used within 2-3 weeks). It can be seen from the TLC images that tubes 81-107 are mainly kestose and tube...

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Abstract

The invention discloses a method for preparing kestose and nystose through yacon. Method of water extraction by alcohol sedimentation is adopted and supernate thereof is taken, concentrated, frozen and dried to obtain faint yellow yacon oligosaccharide. The yacon oligosaccharide is analyzed through high pressure liquid chromatography with a chromatography condition as follows: a chromatographic column is APS-2HYPERSIL, a column temperature is 30 DEG C, a moving phase is acetonitrile-water (75 to 25, V/V), a flow velocity is 0.8ml/min, a sample size is 20 Mul and a differential detector is arranged. The yacon oligosaccharide comprises fructose, glucose, saccharose, kestose, nystose and 1F-Fructofuranosyl nystose with contents respectively as 38,30 percent, 16.44 percent, 14.58 percent, 12.29 percent, 12.17 percent and 6.2 percent. Silicagel column chromatography is implemented on a prepared oligosaccharide sample; glacial acetic acid, chloroform, absolute ethyl alcohol and water with a proportion of 3 to 11 to 11 to 1 are used as an eluant for elution; the eluant is collected in sequence and then is concentrated, frozen and dried to obtain the kestose and nystose. The purity of the kestose and nystose prepared through high pressure liquid chromatography can be more than 90 percent.

Description

technical field [0001] The invention relates to oligosaccharides in yacon, and at the same time, the invention also relates to the qualitative and quantitative analysis of oligosaccharides in yacon and the preparation of kestose and kestetose in fructooligosaccharides. Background technique [0002] Fructose-oligosaccharides are active substances with a higher content in yacon. Fructose-oligosaccharides are composed of kestrose, kestose tetraose, and kestrose pentaose, etc., and have special health functions. Most of the current research focuses on the preparation of high-purity fructooligosaccharides, but there are few studies on the preparation of single components such as kestose and kestose tetraose in fructooligosaccharides, and there is still a lack of mature technology Contents of the invention [0003] The object of the present invention is to provide a kind of yacon oligosaccharide, its extraction method is simple and easy, and can be mass-produced. [0004] Anot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08
Inventor 朱振元连红玉司传领
Owner TIANJIN UNIV OF SCI & TECH
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