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94 results about "HEMOGLOBIN I" patented technology

Hemoglobin is the protein molecule in red blood cells that carries oxygen from the lungs to the body's tissues and returns carbon dioxide from the tissues back to the lungs.

Hemoglobin compositions

The invention provides compositions containing hemoglobin, particularly PEGylated hemoglobin. The PEGylated hemoglobin molecule is capable of transferring oxygen or carbon monoxide bound thereto to a tissue with which it is in proximity. Exemplary PEGylated hemoglobin formulations of the invention are virally inactivated. Various compositions of the invention include deoxygenated hemoglobin, which may be conjugated with one or more water-soluble polymer. PEGylated hemoglobin includes those species in which the iron atom of the hemoglobin molecule is not bound to oxygen or any other species, and hemoglobin molecules in which a species other than oxygen, e.g., carbon monoxide, is bound to the iron atom. The compositions of the invention are formulated as hypo-, iso- or hypertonic solutions of the PEGylated hemoglobin. The compositions are of use to treat and / or ameliorate disease, injury and insult by providing for the oxygenation of tissues and / organs.
Owner:PROLONG PHARMA

Preserving a hemoglobin blood substitute with a transparent overwrap

The invention relates to a method for preserving the stability of a hemoglobin blood substitute comprising maintaining the hemoglobin blood substitute in an atmosphere substantially free of oxygen. The method for preserving a deoxygenated hemoglobin blood substitute comprises maintaining the deoxygenated hemoglobin blood substitute in an oxygen barrier film overwrap package. In one embodiment, the package comprises a transparent laminate material comprising an oxygen barrier layer and a polyolefin layer, wherein the laminant has a thickness of between about 0.001 and about 0.01 inches (or about 0.0254 to about 0.254 millimeters) and an oxygen permeability of less than about 0.01 cubic centimeters per 100 square inches (or about 0.01 cc per 645 square centimeters) over 24 hours at 1 atmosphere and at about 23° C. The oxygen barrier layer comprises ethylene vinyl alcohol. Aso provided herein is a preserved deoxygenated hemoglobin blood substitute comprises a deoxygenated hemoglobin blood substitute and an oxygen barrier film overwrap package. The package comprises a transparent laminant material comprising an oxygen barrier layer and a polyolefin layer. Said oxygen barrier layer has an oxygen permeability of less than about 0.01 cubic centimeters per 100 square inches (or about 0.01 cc per 645 square centimeters) over 24 hours at 1 atmosphere and at about 27° C. room temperature, wherein the deoxygenated hemoglobin blood substitute is sealed within the overwrap package. In one embodiment, the oxygen barrier layer comprises ethylene vinyl alcohol.
Owner:OPK BIOTECH

Method for correcting for blood volume in a serum analyte determination

This invention relates to the measurement of analytes in dried blood samples. It has been discovered by measuring the hemoglobin in the dissolved dried blood sample, an estimate of blood volume may be made and used to make a correction for blood volume. Thus, analytes such as cholesterol, PSA, and other blood analytes can be more accurately determined. The invention further describes a blood collection device and disk material on which blood is dried to provide superior results.
Owner:BIOSAFE LAB

Efficient culture of stem cells for the production of hemoglobin

The present invention describes a serum-free medium that promotes the growth and differentiation of erythroid cells, cells that are highly transducible by a non-viral method and genes which increase the growth of erythroid cells and decrease their dependency on Epo. This invention can be used in the expansion of hematopoietic stem cells to produce cultures of erythroid cells as a source of erythroid-specific proteins such as hemoglobin. Hematopoietic stem cells are cultured ex vivo in a serum-free culture medium with the addition of IL-3, SCF and EPO. Cells transfected with the gene described in the present invention can be cultured in the serum-free culture medium with decreased dependency on Epo and other cytokines, thereby reducing the cost of the production of hemoglobin.
Owner:THERAPURE BIOPHARMA INC

Method of determining that an organ is not suitable for transplantation and using it for testing substances

An organ perfusion apparatus and method monitor, sustain and / or restore viability of organs and preserve organs for storage and / or transport. Other apparatus include an organ transporter, an organ cassette and an organ diagnostic device. The method includes perfusing the organ at hypothermic and / or normothermic temperatures, preferably after hypothermic organ flushing for organ transport and / or storage. The method can be practiced with prior or subsequent static or perfusion hypothermic exposure of the organ. Organ viability is restored by restoring high energy nucleotide (e.g., ATP) levels by perfusing the organ with a medical fluid, such as an oxygenated cross-linked hemoglobin-based bicarbonate medical fluid, at normothermic temperatures. During the period in which the organ is preserved and / or maintained, various drug research and development may be performed on and / or with the organ. The organ may be perfused with a fluid containing a substance such as a test substance to obtain data regarding the organ, the substance and an interaction of the substance and the organ. The data may then be used to ultimately provide information regarding the drugs efficacy in support of regulatory filings for new drugs.
Owner:LIFELINE SCI

Method for testing absolute volume of concentration of oxidized hemoglobin and reduced hemoglobin in human tissue

ActiveCN1911172AHelp to understand blood volumeUnderstanding blood volumeMaterial analysis by optical meansDiagnostic recording/measuringHemoglobin FHEMOGLOBIN I
A method for measuring the absolute concentration of oxygenated and reduced hemoglobin in human tissue features that its sensor is composed of 2 photoelectric receiving tubes and at least 3 light sources with different wavelengths, which are arranged on a straight line at intervals. Said absolute concentration can be calculated out. Said light source can emit respectively red light and near infrared light.
Owner:TSINGHUA UNIV

Nutritive composition for accelerating hemopoietic stem cell proliferation and hemoglobin synthesis

The invention discloses a nutritional composition for promoting the proliferation of hematopoietic stem cells and the synthesis of haemoglobin. The invention is characterized in that the invention comprises raw materials and the proportion by weight as follows: 90 to 110 nucleotide, 20 to 30 parts of arginine, 20 to 30 parts of lysine, 10 to 20 parts of cysteine, 25 to 35 parts of glycin, 20 to 30 parts of histidine, 110 to 130 parts of lecithin, 50 to 70 parts of cephalin, 0.4 to 0.6 parts of vitamin E, 5 to 7 vitamin C, 0.02 to 0.04 parts of folacin, 0.05 to 0.07 parts of vitamin B2, 0.07 to 0.08 vitamin B6, 0.0001 to 0.0002 parts of vitamin B12, 0.5 to 1.5 parts of ferrum, 0.5 to 0.7 parts of zinc, 0.4 to 0.6 parts of manganese, 14 to 16 parts of boxthorn amylase, and 14 to 16 parts of grapestone aqueous extract. The invention can evidently improve the quantity of hemoglobins (Hb), red blood cells (RBC), white blood cells (WBC) and blood platelets (Plt) in the blood, can stimulate the proliferation of the marrow hematopoietic stem cells to lead the hematopoietic stem cells to be evidently increased, can evidently improve the number of mitochondrion in the cell and have obvious recovery function to the damage of liver and spleen.
Owner:珍奥集团股份有限公司

Nitric oxide-blocked cross-linked tetrameric hemoglobin

The present invention includes compositions containing carboxamidomethylated cross-linked hemoglobin where the cysteine moiety of the hemoglobin includes a thiol protecting group and where the hemoglobin has a reduced ability to bind with nitric oxide. Preferably, the hemoglobin is deoxygenated, endotoxin free, and stroma free. The present invention also includes method of preparation, process of preparation and the method of use including supplementing blood volume in mammals and treating disorders in mammals where oxygen delivery agents are of benefit.
Owner:IKOR +1

Reagent for determination of leucocytes and measurement of haemoglobin in a sample of blood

The invention concerns a reagent for determination of leucocytes and measurement of haemoglobin in a sample of blood. This reagent comprises a buffer system that is suited to adjust selectively the pH of the reagent to an acidic value, at least one detergent of cationic type, a nitrogenous compound and, optionally, at least one inorganic salt. This reagent can be used in haematological analyses in human medicine and also permits the identification of a leucocytic subpopulation, in particular the basophil polymorphonuclear leucocytes.
Owner:HORIBA ABX SAS

Carboxymethylated cross-linked tetrameric hemoglobin

The present invention includes compositions containing carboxymethylated cross-linked hemoglobin where the cysteine moiety of the hemoglobin includes thiol protecting group and where the hemoglobin is incapable of binding with the nitric oxide. Preferably, the hemoglobin is deoxygenated, endotoxin free, and stroma free. The present invention also includes method of preparation, process of preparation and the method of supplementing blood in mammals.
Owner:IKOR

Electrochemistry detecting method and testing apparatus of saccharification hemoglobin content

InactiveCN101158691AEasy to combine with electrochemical analysisInexpensive combined with electrochemical analysisMaterial analysis by electric/magnetic meansBiological testingElectrochemical responseHEMOGLOBIN I
The present invention provides an electrochemical detection method for content of glycosylated hemoglobin in the blood, and is characterized in that: whole blood specimen is diluted by combined liquid ranging from 30 times to 70 times and passes through a glycosylated protein recognizer, electric activated small molecules are filled into the effluent liquid from the glycosylated protein recognizer, and electrochemical response of the liquid is detected by chemically modified electrodes after the filling, thereby obtaining the concentration of the blood glucose. The glycosylated protein recognizer is eluted by elute solvent, and the electrochemical response of the elute solvent is detected and the concentration of the glycosylated hemoglobin is obtained, thereby obtaining the content of the glycosylated hemoglobin of the sample after calculation. As the electrochemical detection is simple in operation, lower in cost and rapid in the analysis, the present invention is expected to realize the rapid determination of the clinical glycosylated hemoglobin and the rapid determination of the glycosylated hemoglobin after separation and elution.
Owner:SOUTHEAST UNIV

Targeted oxygen delivery via intravenous or intra-arterial infusion of oxygenated polymerized hemoglobin solutions

InactiveUS20100209532A1Restore oxygenationImprove integrityBiocideMembranesVeinOxygenated Hemoglobin
A method of delivering oxygen to a tissue, a blood vessel, an organ, or a region of an organ, under an ischemic condition, or prophylactically preventing occurrence of an ischemic condition, of a subject, comprising the step of administering to the subject an oxygenated hemoglobin solution, wherein the oxygenated hemoglobin solution includes polymerized hemoglobin, and wherein about 80% by weight, or greater, of the polymerized hemoglobin is oxyhemoglobin.
Owner:HEMOGLOBIN OXYGEN THERAPEUTICS

Occult blood detection in biological samples by laser desorption and matrix-assisted laser desorption/ionization mass spectrometry for biomedical applications

Methods are described for detecting and quantifying occult blood in a biological sample using laser desorption mass spectrometry (LD MS). Biological samples that can be analyzed using various embodiments of the present invention include stool (fecal occult blood, FOB), and any bodily fluid including urine, cerebrospinal fluid and other bodily fluids. If the heme or heme metabolite is bound to protein, the sample is treated with acid before analysis to release the porphyrin. Some of the methods use LD MS with a time of flight analyzer (TOF) to detect and measure unbound heme, other hemoglobin metabolites and other molecules that have a porphyrin-based structure, e.g., bilirubin, biliverdin, protoporphyrin IX, and Zinc protoporphyrin in the biological sample. In other methods, matrix-assisted laser desorption / ionization mass spectrometry (MALDI MS) is used to detect and quantify the individual α- and β-polypeptide chains of hemoglobin.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE

Methods for assaying percentage of glycated hemoglobin

The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase, and peroxidase. The invention provides kits for performing the methods of the invention.
Owner:DIAZYME LAB INC

Use of inositol-tripyrophosphate in treating tumors and diseases

InactiveUS20060106000A1Increasing regulated delivery of oxygenLowering affinity for oxygenBiocidePhosphorous compound active ingredientsDiseaseRadiation sensitivity
Inositol-tripyrophosphate is an allosteric effector of hemoglobin due to its ability to cross the plasma membrane of red blood cells and deliver oxygen to solid tumors, by lowering the oxygen affinity of the hemoglobin of red blood cells. The present invention is directed to the use of inositol-tripyrophosphate to reduce hemoglobin's affinity for oxygen in circulating red blood cells. The present invention is further directed to the use of inositol-tripyrophosphate to inhibit angiogenesis and enhance radiation sensitivity of hypoxic tumors. The present invention is further directed to the use of inositol-tripyrophosphate to enhance PO2 in hypoxic tumors.
Owner:NORMOXYS

Carboxymethylated cross-linked tetrameric hemoglobin

The present invention includes compositions containing carboxymethylated cross-linked hemoglobin where the cysteine moiety of the hemoglobin includes thiol protecting group and where the hemoglobin is incapable of binding with the nitric oxide. Preferably, the hemoglobin is deoxygenated, endotoxin free, and stroma free. The present invention also includes method of preparation, process of preparation and the method of supplementing blood in mammals.
Owner:IKOR

Method for extracting hemoglobin and superoxide dismutase from livestock blood

The invention discloses a method for extracting hemoy lobin and superoxide dismutase from bloods of livestock, which comprises the steps that: after going through an anti-freezing treatment, the fresh bloods of livestock is centrifuged to collect corpuscles, and after hemolysis, heating treatment is carried out so as to extract the hemoy lobin and SOD crude extracted liquid; SOD refined products are obtained after the SOD crude extracted liquid goes through treatments of thermal denaturation, super-filtration concentration, fractionated precipitation of acetone and absorption by ion exchanger. The method of the invention has simple operation and low production cost, is suitable for large-scale industrialized production, and can be used for simultaneously extracting the SOD and hemoy lobin in the bloods of livestock, thereby achieving the highly effective recovery of a plurality of protein resources from the blood of livestock. The extracted hemoy lobin contains salts such as sodium chloride and potassium chloride, etc which are harmless to the human body without containing copper ions. Therefore, the extracted hemoy lobin can be served as food for human beings and livestock, and also can be used for further producing hemoy lobin products such as hemachrome and decolored globin, etc.
Owner:抚顺丹阳生物科技股份有限公司

Cross-linked hemoglobin preparation method and organ perfusate comprising hemoglobin

The invention discloses a cross-linked hemoglobin preparation method, wherein the preparation method comprises the following steps: a, diluting deoxidized hemoglobin, and adding the diluted deoxidizedhemoglobin into an anaerobic reactor; b, arranging an atomizer at the top of the anaerobic reactor, adding a cross-linking agent into the reactor through the atomizer, and carrying out a cross-linking reaction; and c, adding a reducing agent to terminate the cross-linking reaction, and removing the unreacted cross-linking agent and the terminating reducing agent in an ultrafiltration liquid changing manner to obtain the cross-linked hemoglobin. The cross-linked hemoglobin prepared by the preparation method contains low contents of macromolecules (greater than 500 kD) and micromolecules (lessthan 64 kD); by adding the cross-linked hemoglobin into organ perfusate, the phenomena of organ acidosis and lysosomal enzyme activation adverse consequences caused by lack of an oxygen carrying agentin conventional perfusate and cell damage caused by excessive free radicals generated during reperfusion can be solved, and the perfusate is quite simple and convenient to prepare, store, transport and use.
Owner:REDPHARM BEIJING BIOPHARMACEUTICAL INST CO LTD

Broadband illuminator for oximetry, hemoglobin, met-hemoglobin, carboxy-hemoglobin, and other blood component monitoring

An ischemia detection system in two or more somatic measures, collected simultaneously or near-simultaneously, are provided for direct or computational comparison, in which light from light source A (103A) and source B (103B) is detected by a sensor (155), and a difference-weighted value is determined (167), thereby enhancing the value of the spectroscopic measurements over values taken individually and singly.
Owner:J FITNESS LLC

Noninvasive apparatus and method for testing glycated hemoglobin

An apparatus for testing glycated hemoglobin includes a first light measurement device configured to radiate a first light to an object and detect first information about the first light reflected from the object, a second light measurement device configured to radiate a second light to the object and detect second information about the second light reflected from the object, a data extractor configured to extract first extracted data related to hemoglobin from the first information and second extracted data related to glucose from the second information, and a data processor configured to determine information related to glycated hemoglobin from the first extracted data and the second extracted data.
Owner:SAMSUNG ELECTRONICS CO LTD

Blood substitutes

The functional characteristics of heme proteins can be modified to produce hemoglobins that can be used as blood substitutes in different therapeutic applications. Stable polymers of tetrameric hemoglobin, and of myoglobin molecules, are provided for use in the blood substitutes.
Owner:TEMPLE UNIVERSITY +1

Hemoglobin-antioxidant conjugates

There are provided biocompatible chemical compositions having oxygen transporting capability and comprising oxygen transporting molecules chemically bound to antioxidants, to form compositions capable of protecting a mammalian body from oxidative damage. An example of a composition according to the invention is hemoglobin covalently coupled to a 6-hydroxy chroman carboxylic acid, such as Trolox.
Owner:THERAPURE BIOPHARMA INC

Method for measuring glycated hemoglobin

A method for measuring glycated hemoglobin includes hemolyzing a blood sample with a hemolysate; reacting the hemolyzed blood sample with bead conjugates in which beads are conjugated with glycated hemoglobin binding materials; measuring the amount of total hemoglobin in the reacted blood sample; isolating normal hemoglobin from the glycated hemoglobin conjugated with the bead conjugates; measuring the amount of glycated hemoglobin isolated from the normal hemoglobin; and determining the percentage of the glycated hemoglobin in the blood sample on the basis of the measured amounts of total hemoglobin and glycated hemoglobin. The isolation of normal hemoglobin is performed by absorbing the normal hemoglobin using an absorption pad that is a porous pad having pores, each of which has a size greater than the size of the normal hemoglobin and smaller than the size of the bead.
Owner:OSANG HEALTHCARE CO LTD

No-modified hemoglobins and uses therefore

S-nitrosohemoglobin (SNO-Hb) can be formed by reaction of Hb with S-nitrosothiol and by other methods described herein which do not result in oxidation of the heme Fe. Other methods can be used which are not specific only for thiol groups, but which nitrosate Hb more extensively, and may produce polynitrosated metHb as a product or intermediate product of the method. SNO-Hb in its various forms and combinations thereof (oxy, deoxy, met; specifically S-nitrosylated, or nitrosated or nitrated to various extents) can be administered to an animal or human where it is desired to oxygenate, to scavenge free radicals, or to release NO+ groups to tissues. Thiols and / or NO donating agents can also be administered to enhance the transfer of NO+ groups. Examples of conditions to be treated by SNO-Hbs or other nitrosated or nitrated forms of Hb include ischemic injury, hypertension, angina, reperfusion injury and inflammation, and disorders characterized by thrombosis. Further embodiments of the invention are methods for assessing oxygen delivery to the tissues-of a mammal by measuring SNO-Hb and nitrosylhemoglobin in blood.
Owner:DUKE UNIV

Method for improving oxygen transport by stored red blood cells

InactiveUS7267817B2Reduce rateRestored oxygen delivery potentialBiocidePeptide/protein ingredientsMedicineHEMOGLOBIN I
A method of treatment of a patient includes administering to the patient stored red blood cells and a hemoglobin solution. The stored red blood cells and the hemoglobin solution can be administered to the patient simultaneously. Alternatively, the hemoglobin solution can be administered to the patient prior to administrating the stored red blood cells or the stored red blood cells can be administered to the patient prior to administering the hemoglobin solution. A composition of the invention includes stored red blood cells and a hemoglobin solution.
Owner:OPK BIOTECH

Hemolysis reagent composition for hemoglobin a1c quantitative analysis using enzymatic method

Disclosed is a hemolysis reagent composition for the quantitative analysis of glycated hemoglobin (HbAlc) via an enzymatic method. The hemolysis reagent compositions of the present invention containing a zwitterionic surfactant and a nitrous compound considerably accelerate hemolysis rate due to the presence of the zwitterionic surfactant, and also induce the N-terminal β-chain of hemoglobin released by hemolysis to be exposed to the outside of the hemoglobin molecule and assists the participation of a greater number of molecules in the reaction, thereby improving reaction sensitivity and reaction rate of the glycated hemoglobin, and the nitrous compound makes the protein structure of the hemoglobin flexibly modified so that the amino acid sequence of the N-terminal β-chain of hemoglobin can be readily cut off, thereby significantly reducing the entire time required for the measurement and improving the accuracy of the measurement.
Owner:I SENS INC

High-temperature stable oxygen-carrier-containing pharmaceutical composition

A high temperature-stable and highly purified cross-linked (optionally > 70% ss-ss linked) tetrameric hemoglobin with high efficiency of oxygen delivery suitable for use in mammals without causing renal injury and vasoconstriction is provided. The dimeric form of hemoglobin is degenerated and purification processes are performed on red blood cells from whole blood. Controlled hypotonic lysis in an instant cytolysis apparatus prevents lysis of white blood cells. Nucleic acids from white blood cells and phospholipids impurities are not detected. Blocking of reactive sulfhydryl groups by a sulfhydryl reagent is performed in an oxygenated environment. Flowthrough column chromatography removes different plasma protein impurities. N-acetyl cysteine is added to the cross-linked tetrameric hemoglobin to maintain a low level of met-hemoglobin. The stabilized hemoglobin is preserved in an infusion bag with aluminum overwrap to prevent formation of inactive met-hemoglobin from oxygen intrusion. The product finds use in tissue oxygenation and cancer treatment.
Owner:FIRST GAIN INT

Method of preparing controls for glycated hemoglobin s-a1c derived from healthy blood cells

By way of summary, the present invention meets some or all of the above needs by providing in a first aspect a method comprising contacting red blood cells in a suspension medium containing mannose under conditions such that the concentration of S-Alc glycated hemoglobin is increased to greater than about 6 percent by weight of the hemoglobin in the red blood cells. Preferably the suspension medium is an aqueous suspension medium. In preferred embodiments, the method is performed at about ambient temperature, 18° C. to about 23° C.
Owner:STRECK LLC

Glycated hemoglobin detection kit

The invention relates to the biotechnology field, in particular to a glycated hemoglobin detection kit. The glycated hemoglobin detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from rubber latex, a buffer solution A and a surfactant; and the reagent R2 is prepared from an anti-body, a buffer solution B and the surfactant. The kit can eliminate influences ofindividual hemoglobin content difference in glycated hemoglobin detection, is good in stability, and is convenient to use and store.
Owner:SICHUAN MACCURA BIOTECH CO LTD
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