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Electrochemistry detecting method and testing apparatus of saccharification hemoglobin content

A technology of glycosylated hemoglobin and a detection method, which is applied in the field of determination of glycosylated hemoglobin in blood, can solve the problems of expensive instruments and reagents, cumbersome operation steps, and long detection cycle, and achieve the effects of low cost, simple and flexible operation, and fast analysis speed

Inactive Publication Date: 2008-04-09
SOUTHEAST UNIV
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Problems solved by technology

These methods all include a separation step before the photometric quantitative determination of HbA1c content, such as ion-exchange chromatography and electrophoretic separation based on the surface charge difference between HbA1c and Hb, and immunological and boron affinity chromatography methods based on the structural difference between HbA1c and Hb. The detection process requires Multiple elution and special instrument detection, long detection cycle, cumbersome operation steps, expensive instruments and reagents, so the application in the early warning of diabetes and the medium and long-term control of blood sugar levels in diabetic patients is limited

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  • Electrochemistry detecting method and testing apparatus of saccharification hemoglobin content

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Embodiment 1

[0014] Preparation of glycosylated hemoglobin recognizer

[0015] (1) Glass balls with a particle size of 5-10 microns are first activated with 0.1mol / L sodium hydroxide solution for 1 hour, washed with water, then added to 10mmol / L trimethoxy-solution and soaked for 2 hours to obtain trimethyl Oxysilane modified glass spheres, and introduced -CHO active group on the surface of glass spheres. After washing and centrifuging, soak the modified glass beads in 10mmol / L plant lectin ConA (pH 7.4) or aminophenylboronic acid solution for 5 hours, and wash 3 times with pH 7.4 phosphate buffer solution to obtain Plant lectin ConA or aminophenylboronic acid modify the glycosylated hemoglobin recognition medium, fill it into a glass tube, and obtain the glycosylated hemoglobin separation column after solidification.

[0016] (2) According to Si: H 2 O molar ratio 1:4 ratio takes a certain amount of tetraethoxysilane or tetraethoxysilane and the triethoxy-3-silane mixture that accounts ...

Embodiment 2

[0038] An electrochemical detection method for glycosylated hemoglobin content, after diluting the whole blood sample 30-70 times with a binding solution, passing the glycosylated protein identifier, adding electroactive small molecules to the effluent of the whole blood sample of the glycosylated protein identifier, and using The chemically modified electrode measures the electrochemical response of the post-column solution to obtain the concentration a of hemoglobin; the glycosylated protein identifier is eluted with the eluent, and the electrochemical response of the eluent is measured to obtain the concentration b of the glycosylated hemoglobin, and b / (b+a) Obtain the content of glycated hemoglobin in the sample. The glycosylated hemoglobin recognizer uses plant lectin ConA or aminophenylboronic acid modified glass beads, silane hybrid sol or agarose gel as medium. The binding solution is a Heps buffer solution with a pH value of 7.0-8.5. The chemically modified electrod...

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Abstract

The present invention provides an electrochemical detection method for content of glycosylated hemoglobin in the blood, and is characterized in that: whole blood specimen is diluted by combined liquid ranging from 30 times to 70 times and passes through a glycosylated protein recognizer, electric activated small molecules are filled into the effluent liquid from the glycosylated protein recognizer, and electrochemical response of the liquid is detected by chemically modified electrodes after the filling, thereby obtaining the concentration of the blood glucose. The glycosylated protein recognizer is eluted by elute solvent, and the electrochemical response of the elute solvent is detected and the concentration of the glycosylated hemoglobin is obtained, thereby obtaining the content of the glycosylated hemoglobin of the sample after calculation. As the electrochemical detection is simple in operation, lower in cost and rapid in the analysis, the present invention is expected to realize the rapid determination of the clinical glycosylated hemoglobin and the rapid determination of the glycosylated hemoglobin after separation and elution.

Description

technical field [0001] The invention belongs to a technique for measuring glycated hemoglobin in blood, in particular to an electrochemical detection method and a detection device for the content of glycated hemoglobin. Background technique [0002] Diabetes Mellitus (DM) is a disease with abnormal metabolism. Its morbidity and mortality are on the rise, and it has become one of the major diseases that endanger human health after cardiovascular diseases and tumors. Healthy people can digest and decompose carbohydrates into glucose, which enters the blood and is absorbed by cells as raw materials for growth and energy. Insulin is needed to help glucose move from the blood into the cells. Insulin is a hormone secreted by the pancreas that moves glucose across cell membranes where it is converted into energy or other substances for storage. When a healthy person eats, the pancreas can automatically secrete an appropriate amount of insulin to bring glucose from the blood to th...

Claims

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Application Information

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IPC IPC(8): G01N33/72G01N27/413
Inventor 刘松琴吴亚峰
Owner SOUTHEAST UNIV
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