A kind of fingerprint detection method of white ginseng bacteria

A technology of fingerprint spectrum and detection method, applied in the field of fingerprint spectrum detection of white ginseng bacteria, can solve the problem of high cost, and achieve the effects of simple operation, good peak shape and objective conclusion.

Active Publication Date: 2022-05-17
湖南宇山玉月农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The literature "Analysis of Amino Acids and Inorganic Elements in Different Sources of Schizophyllum Fruiting Bodies and Fermented Cultured Mycelia" reported that both wild Schizophyllum fruiting bodies (from Hangzhou) and artificially cultivated Schizophyllum fruiting bodies contained 15 kinds of amino acids, but each The detection needs to use 15 kinds of amino acid reference substances to calculate the content, the cost is high, and other components such as polysaccharides cannot be analyzed as a whole. However, the present invention establishes the fingerprint of white ginseng bacteria, and uses the relative retention time to assign amino acids and other components. Using the relative peak area to control the content of each component, the fingerprint of white ginseng bacteria is of great significance for the quality control of white ginseng bacteria

Method used

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  • A kind of fingerprint detection method of white ginseng bacteria
  • A kind of fingerprint detection method of white ginseng bacteria
  • A kind of fingerprint detection method of white ginseng bacteria

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Experimental program
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Effect test

Embodiment 1

[0030] (1) Screen different chromatographic columns to investigate the number of peaks, resolution and peak shape. (area normalization method, peaks below 0.05% are not counted)

[0031]

[0032] From the results, MCI GEL SCA04 (4.6×150mm) has the most detected peaks and the best resolution, so MCI GELSCA04 was selected

[0033] It is used to develop the detection method of HPLC characteristic fingerprint of white ginseng bacteria.

[0034] (2) Screen different mobile phases to investigate the number of peaks, resolution and peak shape. (area normalization method, peaks below 0.05% are not counted)

[0035]

[0036] From the results, the detection peaks of 5% methanol-water and methanol-0.02N sodium carbonate solution (5:95) systems are basically the same, and the resolution is optimal, so 5% methanol-water is selected as the HPLC characteristic of developing white ginseng bacteria. Mobile phase salts for fingerprint detection.

[0037] (3) Screen different wavelengt...

Embodiment 2

[0041] 1 Methodological investigation 1.1 Chromatographic conditions: MCI GEL SCA04 (4.6×150mm) chromatographic column was used, methanol was used as mobile phase A, 5% methanol-water was used as mobile phase B, gradient elution, flow rate 1.2ml / min, column temperature 30°C, the detection wavelength is 230nm; the number of theoretical plates should not be less than 3000 based on the peak of schizophyllin.

[0042] Gradient elution table:

[0043]

[0044] 1.2 Processing of samples and reference substances

[0045] (1) Preparation of the test solution: Freeze-dry the white ginseng bacteria, crush them, take 5-20g, weigh them accurately, add 80ml of 10% acetic acid to soak for 24h, add 55% methanol for ultrasonic extraction for 1 hour, let cool, and then Weigh the weight, add methanol to make up the weight, shake well, filter, and take the filtrate to get it;

[0046] (2) Preparation of reference substance solution 1: Take 6 mg of schizophyllin reference substance, put it i...

Embodiment 3

[0054] 1. Instrument

[0055] Agilent1260 high performance liquid chromatography (Agilent, USA); PB-10 pH meter (Sartorius), BP-211D analytical electronic balance (Sartorius).

[0056] 2. Test solution

[0057] Methanol (Sigma Company, chromatographically pure), and water was Wahaha purified water.

[0058] 3. Reference substance

[0059] Schizophyllan, valine, isoleucine, leucine, threonine, methionine, lysine, phenylalanine, tryptophan, cysteine, serine, glycine, Tyrosine, Aspartic Acid, Asparagine, Glutamic Acid, Glutamine, Alanine, Arginine, Histidine, Proline.

[0060] 4. Sample

[0061] White ginseng fungus (wild, picked from dead wood on Taoyuan Mountain in Changde).

[0062] 5. Inject each reference substance separately for positioning.

[0063] 6. Establishment of fingerprints and marking of common peaks

[0064] The white ginseng bacterium is prepared need testing solution by the method under "1.2" item in embodiment 2 respectively, is analyzed by the chromato...

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Abstract

The invention discloses a fingerprint detection method of white ginseng fungus, which uses high-pressure liquid chromatography to measure the fingerprint with reference to a schizophyllin reference substance. The invention establishes the HPLC characteristic fingerprint of the white ginseng fungus, overcomes the singleness and one-sidedness of the existing quality standards, and can fully reflect the quality information of the white ginseng fungus, thereby achieving the purpose of more comprehensive and effective quality control of the white ginseng fungus. The white ginseng bacteria fingerprint spectrum obtained by the method of the invention has many peaks, good peak shape, easy identification, high similarity, accuracy and reliability.

Description

technical field [0001] The invention belongs to the field of quality analysis, and in particular relates to a fingerprint detection method of white ginseng bacteria. Background technique [0002] White ginseng fungus (Schizophyllum communer), also known as schizophyllum communer, white fungus (Yunnan), tree flower (Shaanxi), cockscomb (Hunan), chicken feather fungus (Hubei), white ginseng mushroom (Henan), belongs to Basidiomycotina . White ginseng fungus is a rare mountain delicacy that is used both as food and medicine, and enjoys a high reputation in domestic and foreign markets. [0003] White ginseng, gills narrow, radiating from base, stipe short or absent. Mushrooms have tough physique, fragrant taste, delicious and refreshing, and rich nutrition. According to the analysis of artificially cultivated white ginseng bacteria by the Testing Center of Yunnan Academy of Agricultural Sciences, the total content of 8 essential amino acids in white ginseng bacteria reaches ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8686
Inventor 曾灿丽卢洪成
Owner 湖南宇山玉月农业科技有限公司
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