Method for catalytic production of fructo-oligosaccharides by aspergillus oryzae whole cells
The technology of cane fructooligosaccharide and whole cell catalyst is applied in the field of oligosaccharide, which can solve the problems of harsh production environment and conditions, complicated separation and purification process, restricting industrial application, etc., and achieves easy operation, simple process, and reduced fermentation. Effects of the tank and its accessories
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Embodiment 1
[0029] The first step is to inoculate the spores of Aspergillus oryzae CICC 2134 (Aspergilusoryzae2134, preserved by the China Industrial Microorganism Culture Collection Management Center, address: No. 32 Xiaoyun Road, Chaoyang District, Beijing, Zip Code: 100027) into the liquid seed medium. 1×10 5 A / L, at 33 ℃, the thalline solution after 16h of cultivating on the shaker of rotating speed 150r / min is made seed liquid; The formula of described liquid seed culture medium is: 20g / L sucrose, 30g / L corn flour, 100g / L L yeast extract powder, 2g / L NaNO 3 ;Stir and dissolve evenly, then sterilize, and cool for later use;
[0030] Step 2: Inoculate the seed solution obtained in the first step into the liquid medium for producing enzyme inducer at a ratio of 5:100 by volume, and cultivate the bacteria for 24 hours at 33°C on a shaker with a rotation speed of 150rpm The solution is made into a whole-cell thalline solution; the liquid medium containing an enzyme inducer is: 70g / L suc...
Embodiment 2
[0036] The first step is to inoculate the spores of Aspergilus oryzae CICC 2134 (Aspergilus oryzae 2134, preserved by China Industrial Microorganism Culture Collection Management Center, address: No. 32 Xiaoyun Road, Chaoyang District, Beijing, Zip Code: 100027) into the liquid seed medium, The inoculum size is 1×10 10 A / L, at 28 ℃, the bacterial cell solution after 24 hours of cultivating on a shaker with a rotating speed of 100r / min is made into a seed liquid; the formula of the liquid seed medium is: 50g / L sucrose, 7g / L malt powder, 10g / L L peptone, 3g / L NaCl; stir and dissolve evenly, use for sterilization, cool and set aside;
[0037] The second step: Inoculate the seed solution obtained in the first step into the liquid medium for producing enzyme inducer at a volume ratio of 10:100, and cultivate the bacteria for 16 hours at 28°C with a rotation speed of 100rpm The solution is made into a whole-cell thalline solution; the liquid culture medium containing an enzyme indu...
Embodiment 3
[0042] The first step is to inoculate the spores of Aspergilus oryzae CICC 2134 (Aspergilus oryzae 2134, preserved by China Industrial Microorganism Culture Collection Management Center, address: No. 32 Xiaoyun Road, Chaoyang District, Beijing, Zip Code: 100027) into the liquid seed medium, The inoculum size is 1×10 6 A / L, at 25 ℃, the bacterial cell solution after 20h of cultivating on the shaker of 120r / min rotating speed is made seed liquid; The formula of described liquid seed culture medium is: 30g / L sucrose, 5g / L malt powder, 7g / L L peptone, 50g / L yeast extract, 1.5g / L NaCl, 1.5g / L NaNO 3 ;Stir and dissolve evenly, then sterilize, and cool for later use;
[0043] The second step is to inoculate the seed solution obtained in the first step into the liquid medium for producing enzyme inducer with a volume ratio of 8:100, and cultivate the bacterial cell solution for 20 hours at 25°C on a shaker with a rotation speed of 300rpm Make whole cell thalline solution; Described ...
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