Method for preparing anti-oxidative peptide from aspergillus oryzae fermented shrimp shells
An antioxidant peptide, Aspergillus oryzae technology, applied in the field of preparation of antioxidant peptides, can solve the problems of high energy consumption, high cost, non-recyclable and reusable enzyme inactivation, and achieves low reaction temperature, reduced energy consumption, and high polypeptide content. Effect
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[0029] Embodiment 1. A method for preparing antioxidant peptides by fermenting shrimp shells with Aspergillus oryzae, the following steps are carried out successively
[0030] 1) Cultivation of Aspergillus oryzae: Pour 8-10ml of the prepared Chardonnay medium into a petri dish, inoculate 1 ring (inoculation ring) of Aspergillus oryzae, and cultivate at 25-28°C. After Aspergillus oryzae overgrown the petri dish (about 120 hours), add 10 ml of distilled water and mix well to obtain Aspergillus oryzae suspension, and take 1 ml for fermentation inoculation.
[0031] The Aspergillus oryzae was purchased from the China Culture Collection Center, serial number: 3.0951; the name was Rhizopus oligoides Dale var.
[0032] 2), prepare the fermentation base material according to the mass-volume ratio of shrimp shell: glucose: water of 5:1.5:30 (g / g / ml), then add 1ml of Aspergillus oryzae suspension, and ferment at a temperature of 25-28°C for 30 days ;
[0033] 3), the fermentation brot...
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