Aspergillus oryzae strain YSY035 and isolating and screening method and application of selenium-enriched Aspergillus oryzae

A YSY035, separation and screening technology, applied in the field of food additives, can solve the problems of low selenium enrichment, less introduction of fermentation methods, etc.

Inactive Publication Date: 2016-08-31
BEIJING YUANSHENGYUAN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN1632128A provides a method for preparing organic selenium from Aspergillus oryzae, but its selenium-rich amount is low, and the fermentation method is less introduced

Method used

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  • Aspergillus oryzae strain YSY035 and isolating and screening method and application of selenium-enriched Aspergillus oryzae
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  • Aspergillus oryzae strain YSY035 and isolating and screening method and application of selenium-enriched Aspergillus oryzae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Isolation and screening method and identification of Aspergillus oryzae YSY035 strain:

[0090] (1) Strain isolation: Take 1g of rice wine koji collected from a food factory, put it into 10mL sterile water, shake and mix well, and prepare 10 -1 Sample diluent; then use serial dilution method to serially dilute, respectively prepare 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 For different concentrations of spore suspension, draw 0.2mL of different concentrations of spore suspension and spread it evenly on the Martin's medium plate, incubate at 25°C for 4 days, observe and record the growth of the colony;

[0091] Among them, the composition of Martin's medium is: KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 10g, 1% Bengal red aqueous solution 3.3mL, agar 15-20g, water 1000mL, pH value is natural; add 0.3mL of 1% streptomycin solution to every 100mL medium before use;

[0092] (2) Strain purification: Inoculate the single colony grown on the Martin's medium ...

Embodiment 2

[0099] The isolation and screening method of Aspergillus oryzae YSY033:

[0100] (1) Strain isolation: Take 1 g of the sauce collected from the food factory, put it into 10 mL of sterile water, shake and mix well, and prepare 10 -1 Sample diluent; then use serial dilution method to serially dilute, respectively prepare 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 For different concentrations of spore suspension, draw 0.2mL of different concentrations of spore suspension and evenly spread it on the Martin's medium plate, culture it at 25°C for 6 days, observe and record the growth of the colony;

[0101] Among them, the composition of Martin's medium is: KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 10g, 1% Bengal red aqueous solution 3.3mL, agar 15-20g, water 1000mL, pH value is natural; add 0.3mL of 1% streptomycin solution to every 100mL medium before use;

[0102] (2) Strain purification: inoculate the single colony grown on the Martin's medium plate on the slope...

Embodiment 3

[0108] The isolation and screening method of Aspergillus oryzae YSY036:

[0109] (1) Strain isolation: Take 1 g of koji and soy sauce koji collected from a food factory, put it into 10 mL of sterile water, shake and mix well, and prepare 10 -1 Sample diluent; then use serial dilution method to serially dilute, respectively prepare 10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 For different concentrations of spore suspension, draw 0.2mL of different concentrations of spore suspension and evenly spread it on the Martin's medium plate, incubate at 26°C for 4 days, observe and record the growth of the colony;

[0110] Among them, the composition of Martin's medium is: KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 10g, 1% Bengal red aqueous solution 3.3mL, agar 15-20g, water 1000mL, pH value is natural; add 0.3mL of 1% streptomycin solution to every 100mL medium before use;

[0111] (2) Strain purification: inoculate the single colony grown on the Martin's medium plate on...

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Abstract

The invention relates to an Aspergillus oryzae strain YSY035 and an isolating and screening method and application of selenium-enriched Aspergillus oryzae. The Aspergillus oryzae strain YSY035 has been preserved in China General Microbiological Culture Collection Center (CGMCC for short) on May 11, 2016, with a preservation number of CGMCC NO. 12378. By virtue of isolation, purification, primary screening and secondary screening on the Aspergillus oryzae strain, the selenium-enriched Aspergillus oryzae is obtained; and by virtue of scale production on the selenium-rich Aspergillus oryzae, a selenium-enriched product with a selenium content of 8.0mg / g-11.0mg / g and an organic selenium content of 7.0mg / g-10.5mg / g is obtained. The selenium-enriched Aspergillus oryzae provided by the invention is used as an additive of foods and health products for replacing common Aspergillus oryzae, so that selenium can be continuously supplemented, and diseases caused by selenium deficiency can be effectively prevented and treated.

Description

technical field [0001] The invention relates to the technical field of food additives, in particular to a method for separating and screening Aspergillus oryzae strain YSY035 and selenium-enriched Aspergillus oryzae and its application. Background technique [0002] As an essential trace element for humans and animals, selenium has a variety of biological functions, such as participating in a variety of enzymatic reactions, promoting the decomposition of toxic peroxides in the metabolic process, enhancing the body's immunity, inhibiting the absorption of toxic substances in the body, and anti-oxidation. cancer etc. The World Health Organization recommends that the human body supplement 200 μg of selenium per day. However, insufficient and unbalanced selenium intake is common in China, and the phenomenon of selenium deficiency is relatively common. About 2 / 3 of the 22 provinces and cities in the country are deficient in selenium. The lack of selenium can cause many diseases,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L33/10A61K36/06A61P3/02C12R1/69
CPCA61K36/06C12N1/14C12N1/145C12R2001/69
Inventor 于赞毛亮
Owner BEIJING YUANSHENGYUAN SCI & TECH DEV
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