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Method for increasing selenium enrichment rate of cordycep smilitaris(Vuill.)Fr. by utilizing illumination

A technology for Cordyceps militaris and selenium enrichment, which is applied in the field of microbial culture, can solve problems such as rare effects of selenium enrichment in Cordyceps militaris, and achieve the effects of avoiding inhibition, improving selenium enrichment rate, and improving selenium enrichment efficiency.

Active Publication Date: 2019-07-05
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current research on selenium-enriched Cordyceps militaris is mostly in the aspects of medium composition and subsequent extraction, but there are few studies on the influence of culture conditions, especially light conditions, on selenium-enriched Cordyceps militaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0042] After the liquid mycelium of Cordyceps militaris was inserted into the basic medium, it was cultivated in a shaker at 20°C and 160r / min under dark conditions, and cultivated for a period of time until the mycelial activity was the strongest;

[0043] Then, adjust the pH of the medium to 5, and add Na 2 SeO 3 Adjust the concentration to 25 μg / ml for selenium-enriched culture as a control group;

[0044] Another group of Cordyceps militaris liquid mycelium was inserted into pH=5, and the concentration of 25 μg / ml Na was added 2 SeO 3 In the basal medium, and under dark conditions, 20 ° C, 160r / min shaker culture for the same time, as a treatment group;

[0045] There were at least three control groups for each group.

[0046] Continue to culture the control group and the treatment group at 20 ℃, 160r / min shaker for a period of time, and measure the effect of Cordyceps militaris mycelia on Na 2 SeO 3 total absorption.

[0047] Measurement result shows: the mycelia g...

experiment example 2

[0052] Put the liquid mycelium of Cordyceps militaris into the basic medium, and culture it on a shaker at 20°C and 160r / min in the dark, and cultivate it for a period of time until the mycelium activity is the strongest, adjust the pH of the medium to 5 and add Na 2 SeO 3 Adjust the concentration to 25 μg / ml for selenium-enriched culture;

[0053] Among them, one group was cultured on a shaker in the dark as a control group, and the other group was cultured on a shaker under 600 lx white light irradiation under the same other conditions as a treatment group. There were at least three control groups for each group.

[0054] Continue 20 ℃, 160r / min shaker culture 40h, measure the mycelia of Cordyceps militaris to Na 2 SeO 3 total absorption.

[0055] The measurement results showed that: the hyphae of the treatment group were sensitive to Na 2 SeO 3 The total absorption of 1.519±0.250mg, selenium enrichment rate is 60.2%;

[0056] Na 2 SeO 3 The total absorption of sele...

experiment example 3

[0061] Put the liquid mycelium of Cordyceps militaris into the basal medium, and cultivate it on a shaker at 20°C and 160r / min in the dark, and cultivate it for a period of time until the mycelium activity is the strongest, adjust the pH of the medium to 5, and add Na 2 SeO 3 Adjust the concentration to 25 μg / ml for selenium-enriched culture;

[0062] A group of cultured on a shaking table in the dark was used as a control group, and a group of other conditions were the same and placed on a shaking table under 30 lx blue light irradiation as a treatment group. There were at least three control groups for each group.

[0063] Continue 20 ℃, 160r / min shaker culture 40h, measure the mycelia of Cordyceps militaris to Na 2 SeO 3 total absorption.

[0064] The measurement results showed that: the hyphae of the treatment group were sensitive to Na 2 SeO 3 The total amount of absorption is 1.950±0.110mg, and the selenium enrichment rate is 77.5%;

[0065] Na 2 SeO 3 The total...

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Abstract

The invention provides a method for increasing the selenium enrichment rate of cordycep smilitaris(Vuill.)Fr. by utilizing illumination. The method comprises the steps of culturing cordycep smilitaris(Vuill.)Fr. mycelia under a dark condition and then culturing the cordycep smilitaris(Vuill.)Fr. mycelia under a blue light illumination condition, wherein during culturing under the blue light illumination condition, culturing is performed under the blue light illumination condition at a liquid mycelium culturing stage and / or a color conversion stage in a solid culture medium. The blue light is used for replacing traditional white light as a scattering light source, correspondingly the yield of cordycep smilitaris(Vuill.)Fr. stromata can be increased, and the selenium enrichment efficiency ofcordycep smilitaris(Vuill.)Fr. can be improved. According to the method, blue light illumination is utilized, and the inhibition effect of white light which is adopted as a light source in a traditional method on absorption of inorganic selenium in cordycep smilitaris(Vuill.)Fr. can be avoided.

Description

technical field [0001] The invention relates to the field of microorganism cultivation, in particular to a method for increasing the selenium enrichment rate of Cordyceps militaris by using light. Background technique [0002] Selenium is an essential trace element for the human body, and it is also an internationally recognized human immune activator. Therefore, selenium supplementation has become an important issue related to human health. Due to its low physiological activity and certain biological toxicity, the use of inorganic selenium as a selenium supplement is decreasing, and more and more people are supplementing selenium through the intake of organic selenium. [0003] Selenium-enriching bacteria is a product that organically combines inorganic selenium with edible fungi. Through the absorption and fermentation of edible fungi, it can convert inorganic selenium into organic selenium, making selenium easier for the human body to absorb and utilize. [0004] Cordyc...

Claims

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Application Information

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IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 王新风顾文智胡卫成陈倩倩胡烨烨
Owner HUAIYIN TEACHERS COLLEGE
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