A method for improving the selenium enrichment rate of Cordyceps militaris by using light
A technology of Cordyceps militaris and selenium enrichment, which is applied in the field of microbial cultivation, can solve problems such as the rare influence of Cordyceps militaris selenium enrichment, and achieve the effects of avoiding inhibition, improving the efficiency of selenium enrichment, and increasing the rate of selenium enrichment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
experiment example 1
[0042] After the liquid mycelium of Cordyceps militaris was inserted into the basic medium, it was cultivated in a shaker at 20°C and 160r / min under dark conditions, and cultivated for a period of time until the mycelial activity was the strongest;
[0043] Then, adjust the pH of the medium to 5, and add Na 2 SeO 3 Adjust the concentration to 25 μg / ml for selenium-enriched culture as a control group;
[0044] Another group of Cordyceps militaris liquid mycelium was inserted into pH=5, and the concentration of 25 μg / ml Na was added 2 SeO 3 In the basal medium, and under dark conditions, 20 ° C, 160r / min shaker culture for the same time, as a treatment group;
[0045] There were at least three control groups for each group.
[0046] Continue to culture the control group and the treatment group at 20 ℃, 160r / min shaker for a period of time, and measure the effect of Cordyceps militaris mycelia on Na 2 SeO 3 total absorption.
[0047] Measurement result shows: the mycelia g...
experiment example 2
[0052] Put the liquid mycelium of Cordyceps militaris into the basic medium, and culture it on a shaker at 20°C and 160r / min in the dark, and cultivate it for a period of time until the mycelium activity is the strongest, adjust the pH of the medium to 5 and add Na 2 SeO 3 Adjust the concentration to 25 μg / ml for selenium-enriched culture;
[0053] Among them, one group was cultured on a shaker in the dark as a control group, and the other group was cultured on a shaker under 600 lx white light irradiation under the same other conditions as a treatment group. There were at least three control groups for each group.
[0054] Continue 20 ℃, 160r / min shaker culture 40h, measure the mycelia of Cordyceps militaris to Na 2 SeO 3 total absorption.
[0055] The measurement results showed that: the hyphae of the treatment group were sensitive to Na 2 SeO 3 The total absorption of 1.519±0.250mg, selenium enrichment rate is 60.2%;
[0056] Na 2 SeO 3 The total absorption of sele...
experiment example 3
[0061] Put the liquid mycelium of Cordyceps militaris into the basal medium, and cultivate it on a shaker at 20°C and 160r / min in the dark, and cultivate it for a period of time until the mycelium activity is the strongest, adjust the pH of the medium to 5, and add Na 2 SeO 3 Adjust the concentration to 25 μg / ml for selenium-enriched culture;
[0062] A group of cultured on a shaking table in the dark was used as a control group, and a group of other conditions were the same and placed on a shaking table under 30 lx blue light irradiation as a treatment group. There were at least three control groups for each group.
[0063] Continue 20 ℃, 160r / min shaker culture 40h, measure the mycelia of Cordyceps militaris to Na 2 SeO 3 total absorption.
[0064] The measurement results showed that: the hyphae of the treatment group were sensitive to Na 2 SeO 3 The total amount of absorption is 1.950±0.110mg, and the selenium enrichment rate is 77.5%;
[0065] Na 2 SeO 3 The total...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com