Saccharomyces longidosporum and its selenium-enriched culture method and application

A technology of yeast long spore and a culture method, which is applied in the field of microorganisms and achieves the effects of high selenium enrichment efficiency, good application prospects and good selenium enrichment performance.

Active Publication Date: 2017-09-01
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no public report on the related research of Saccharomyces longans as a selenium-enriched yeast

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  • Saccharomyces longidosporum and its selenium-enriched culture method and application
  • Saccharomyces longidosporum and its selenium-enriched culture method and application
  • Saccharomyces longidosporum and its selenium-enriched culture method and application

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Embodiment 1

[0028] real materials and methods

[0029] 1.1 Test material

[0030] 1.1.1 Strains

[0031] In the present invention, the inventor uses a selective medium to screen the Loudtia longidosporum isolated from the intestinal tract of freshwater fish, and analyzes the ITS sequence and hsp78 gene. The new Lodderomyces elongisporus is classified as Lodderomyces elongisporus, numbered SCLE01, and has been deposited in the China Center for Type Culture Collection (CCTCC) on May 15, 2015, with the preservation number CCTCC NO: M 2015301.

[0032] The morphological characteristics of the bacterial strain of L. elongisporus SCLE01 (L.elongisporus SCLE01, preservation number is CCTCCNO: M 2015301) described in the present invention are: the bacterial colony is milky white, slightly rough, and after overnight cultivation on solid medium, the bacterial colony is 0.5- 1mm, the surface is relatively smooth, the edges are neat, the colonies are raised, and the density is high; after static cu...

Embodiment 2

[0056] This example evaluates its selenium-enriching performance by studying the selenium-tolerant ability and selenium-enriched fermentation conditions of freshwater fish-derived Loudtia longans, and obtains the concentration of selenium in the medium, the way of adding selenium, the inoculation amount and the culture time through a single factor test The level range of 4 factors provides a basis for evaluating the feasibility of freshwater fish-sourced Loudia longans as a selenium-enriched yeast strain.

[0057] 2.1 Determination method of biomass and selenium content

[0058] 2.1.1 Biomass measurement

[0059] After the shaking culture was completed, the culture medium was taken, centrifuged at 6000rpm for 5min, and the bacterial cell precipitate was washed 3 times with distilled water, and the bacterial cell was collected, dried at 90°C to constant weight, and then weighed.

[0060] 2.1.2 Determination of selenium content

[0061] Se(Ⅳ) can catalyze potassium chlorate to...

Embodiment 3

[0083] 3.1 Test material

[0084] The yeast used in this test is the freshwater fish intestinal strain that has been isolated and identified: Saccharomyces longus sporogenes (SCLE01, preservation number CCTCC NO.M 2015301). Japanese eel juveniles and complete feed (as basic feed) were purchased from Dongguan Yinhua Eel Breeding Co., Ltd. Lysozyme (LZM) was purchased from Nanjing Jiancheng Bioengineering Institute.

[0085] 3.2 Test method

[0086] 3.2.1 Preparation of selenium-enriched yeast

[0087]Prepare selenium-enriched yeast according to the previous test results, and the specific conditions are as follows: 10% inoculum size, 20 μg / mL selenium concentration (final concentration in the medium), added in 3 times (6h, 9h, 12h) in the logarithmic phase , the amount added in turn is 25%, 25%, 50%, 30°C, 190rpm shaking culture for 36h, to obtain selenium-enriched Loudia longans. Then the biomass of selenium-enriched yeast and the selenium content of bacteria were measured,...

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Abstract

The invention relates to a Lodderomyces elongisporus, whose preservation number is CCTCC NO: M 2015301. The selenium-enriched culture method of Lodderomyces elongisporus (Lodderomyces elongisporus) comprises the following steps: inoculating the above-mentioned Lodderomyces elongisporus in the YEPD medium for cultivation, 5%-10% inoculation amount; Add selenium three times at 6h, 9h, and 12h. The final concentration of selenium in the medium is 10-20μg / mL, and culture for 24h-48h. The Loudtia longidosporum yeast described in the present invention has high selenium-enriching efficiency and good selenium-enriching performance, has the feasibility of producing organic selenium, can be used as a selenium-enriched yeast strain, and has a good application prospect in the product development of organic selenium animal feed additives .

Description

technical field [0001] The invention relates to the field of microbes, in particular to a Loudtia longidosporum and its selenium-enriched culture method and application. Background technique [0002] Selenium is one of the essential trace elements for aquatic animals. It has the functions of anti-oxidation, enhancing the body's immunity, regulating the body's metabolism, enhancing reproductive ability, and reducing certain heavy metal poisoning. Selenium deficiency will reduce the activity of glutathione peroxidase (GSH-Px) in fish, weaken the body's antioxidant capacity, lead to the accumulation of free radicals in the body, and reduce the ability to prevent and resist diseases. Selenium can significantly improve the growth of aquatic animals and enhance the resistance of aquatic animals, and the effect of organic selenium is better than that of inorganic selenium. [0003] Yeast is the most widely used and safest because of its fast growth, convenient cultivation, protein...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23K20/10A23K50/80
CPCC12N1/16C12N1/145C12R2001/645Y02A40/818
Inventor 郝乐柯浩刘振兴马艳平梁志凌马江耀
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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