Epicoccum purpurascens and Aspergillus spp. coculture method, and compound obtained through method

A technology of Aspergillus and Epicoccus niger, applied in the field of microbiology, can solve the problems of limiting the discovery of new compounds and the difficulty of discovering new compounds

Active Publication Date: 2017-05-17
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the deepening of research, a large number of compounds obtained by the traditional screening mode of discovering natural products from fungi have been repeatedly discovered. Under conventional laboratory culture conditions, Aspergillus can only produce limited metabolites,

Method used

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  • Epicoccum purpurascens and Aspergillus spp. coculture method, and compound obtained through method
  • Epicoccum purpurascens and Aspergillus spp. coculture method, and compound obtained through method
  • Epicoccum purpurascens and Aspergillus spp. coculture method, and compound obtained through method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The medium used in the present invention for the activation of E. niger strain strains is a PDA medium, which is sterilized under high pressure at 121° C. for 20-30 minutes. Invert a 90mm petri dish, take E. niger mycelium and inoculate it, culture it statically at 25°C for 3 days to 7 days, store it at 4°C, and use it for cultivation and inoculation.

Embodiment 2

[0043] The used Aspergillus strain activation medium of the present invention is GMM medium, and the described solid medium of every 1L is prepared as follows: glucose 10g, nitrate mother liquor 50mL, trace element mother liquor 1mL, yeast extract 1g, agar 15-20g , add the above ingredients in sequence, and finally add distilled water to the final volume to 1L, adjust the pH value to 6.5, and sterilize at 121°C for 20-30 minutes. Invert a 90mm petri dish, streak and inoculate, and culture at 37°C for 3 to 4 days. Collect spores in sterile water, collect spores from each plate, make a spore suspension with a final volume of 2 mL, and store at 4°C for culture inoculation.

[0044] Wherein, the nitrate mother liquor preparation (1L) method is as follows: sodium nitrate NaNO 3 120g, potassium chloride KCl 10.4g, magnesium sulfate MgSO 4 ·7H 2 O 10.4 g, dipotassium hydrogen phosphate K 2 HPO 4 ·3H 2 O 30.4g, add the above ingredients in order, and finally add distilled water...

Embodiment 3

[0047] The used coccus niger and aspergillus co-cultivation method of the present invention are: coccus niger and aspergillus nidulans, aspergillus terreus and aspergillus oryzae are simultaneously inoculated onto the same petri dish that PDA medium is poured down respectively, co-cultivate. The cultivation temperature is 25° C. to 28° C., and the culture is static; after a period of cultivation, the growth of Aspergillus niger close to the side of E. niger is significantly inhibited, and the Aspergillus spores are obviously discolored; the cultivation time is 4 days to 7 days.

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Abstract

The invention relates to an Epicoccum purpurascens and Aspergillus spp. coculture method. The coculture method allows aspergillus to generate secondary metabolites which are lowly yielded or not generated in the single culture process. The method comprises the following steps: 1, respectively inoculating an activation medium with Epicoccum purpurascens and Aspergillus spp. to activate strains; 2, inoculating a solid medium in a same culture container with the activated Epicoccum purpurascens and Aspergillus spp., and carrying out stationary culture; or inoculating a liquid medium in the same culture container with the activated Epicoccum purpurascens and Aspergillus spp., and carrying out shaking culture; and 3, collecting the Aspergillus spp. and the media when Aspergillus spp. spores in the solid medium become yellow in order to obtain a product. The invention also relates to secondary metabolite produced through the coculture method.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a method for co-cultivating P. niger and Aspergillus fungi and the obtained compound. Background technique [0002] Aspergillus spp. is an important class of filamentous fungi. Many groups in this genus are closely related to human life and health. They are widely used in traditional wine making sauces and modern food processing industries, such as: Aspergillus ferments soybeans with yeast, bacteria or combined bacteria to produce soybean paste and soy sauce; mixed fermented rice is used to produce rice wine; it is used to accelerate the ripening of cheese to make cheese have a special flavor, and it can also be used to smoke ham, sausage, etc. food. Over the years, a large number of secondary metabolites have been found in Aspergillus fungi, which have important research and application value. Some of them are highly carcinogenic mycotoxins, such as: Aflatoxins and Sterigmatocystin pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P7/24C12P7/26C12P17/06C12P17/04C07C47/575C07C50/34C07D311/92C07D307/89C12R1/66C12R1/69
Inventor 尹文兵李伟吴凡吴广畏
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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