Method for cultivating aspergillus versicolor HY12 strains

A technology of Aspergillus versicolor and its cultivation method, applied in the biological field, can solve the problems of loss of commodity value, harm, production damage, etc., and achieve the effects of high teratogenicity rate and lethality rate, and fast growth and reproduction speed

Active Publication Date: 2019-03-22
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Spodoptera litura enters gluttony after the 4th instar and bites the leaves, leaving only the main veins. On the cabbage, the larvae can also drill into the leaf balls to cause harm, eat up the interior, and excrete feces, causing pollution and reducing it. loss of commodity value
Spodoptera litura pests occur all over the country, causing great damage to production

Method used

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  • Method for cultivating aspergillus versicolor HY12 strains
  • Method for cultivating aspergillus versicolor HY12 strains
  • Method for cultivating aspergillus versicolor HY12 strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Screening of Aspergillus versicolor HY12 strain

[0034] 47 soil samples were collected from the Spodoptera litura infested areas in southern Hunan tobacco fields, including 25 soil samples in Jianghua, Yongzhou, 16 soil samples in Hengyang, 4 soil samples in Jiangyong, and 2 soil samples in Ningyuan. Among them, the 5-point sampling method is used to collect soil samples, and the center distance between the two tobacco plants is selected as the soil sampling point, and the soil samples with a length of 10 cm, a width of 10 cm, and a depth of 5 cm are cut down with a soil shovel and put into a sterile self-sealing plastic bag. Take it back to the laboratory and store it in the refrigerator for separation. The time, location and related information of sample collection shall be recorded in detail, and different soil samples shall be marked separately.

[0035] The retrieved tobacco field soil samples were crushed under aseptic conditions and fully mixed, then fully mixe...

Embodiment 2

[0054] Cultivation of Aspergillus versicolor HY12 strain

[0055] The Aspergillus versicolor HY12 strain obtained in Example 1 was identified, and the Aspergillus versicolor HY12 had a high similarity with multiple strains of Aspergillus, and the similarity coefficient with Aspergillus versicolor reached 99%. According to the rDNA ITS sequence, combined with its morphology The strain HY12 was identified as Aspergillus versicolor, wherein the rDNA ITS segment size of Aspergillus versicolor was about 500bp; SEQ ID NO.1.

[0056] The Aspergillus versicolor HY12 strain was cultured on a fungal medium (4 parts of glucose, 1 part of peptone, 1 part of yeast extract powder, 2 parts of agar, 0.2 part of gentamicin, 100 parts of water) until sufficient sporulation, and picked Conidia powder, utilize 0.05% Tween80 solution to prepare concentration and be 10 8 spore suspension per mL; inoculate 10ml of spore suspension into 1L fermentation medium (glucose 40g, peptone 10g, yeast extract...

Embodiment 3

[0058] In the present embodiment, the cultivation temperature of Aspergillus versicolor HY12 strain in the fermentation medium is 15, 20, 22, 25, 28, 30 and 35 respectively, and the OD is measured with a spectrophotometer 600nm value and record the results, each group was repeated 3 times, and the thalline growth in each fermentation medium was counted to reach the maximum value, and other technical characteristics were the same as in Example 2. The result is as figure 2 shown by figure 2 It can be seen that the temperature of the fermentation medium is the maximum at 28 ° C, OD 600nm was 0.833; when the temperature was higher than 30℃, the growth of Aspergillus versicolor HY12 strain was significantly inhibited.

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Abstract

The invention provides a method for cultivating aspergillus versicolor HY12 strains. The method comprises steps of preparation of aspergillus versicolor HY12 strain conidium suspension and fermentation cultivation. According to the method for cultivating aspergillus versicolor HY12 strains provided by the invention, by researching growth and propagation habits of the aspergillus versicolor HY12 strains, the aspergillus versicolor HY12 can achieve an extremely high growth and propagation rate within a short time by utilizing the cultivation method in the invention, and lots of conidiums can beproduced. The method has a high teratogenesis rate and fatality rate on prodenia litura, lays an experimental foundation for later control of the prodenia litura, and provides favorable reference forcontrol of other injurious insects in the field.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for cultivating Aspergillus versicolor HY12 strain. Background technique [0002] Since the invention of chemical pesticides, chemical pesticides have grown at an astonishing rate of 5% per year. Although in the past few decades, chemical pesticides have played an important role in increasing agricultural production and pest control, the extensive use of chemical pesticides has brought about serious "3R" problems (namely, resistance to pesticides, residence of pesticide residues, and Rampant Resurgence) has also brought a series of ecological and environmental problems. The long-term and extensive use of chemical pesticides has led to an increase in pesticide-resistant pests, especially in the past 10 years, the resistance of cotton bollworms, aphids, diamondback moths, Spodoptera litura, and spider mites to pyrethroids and organophosphorus chemical pesticides has increased hund...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/66
CPCC12N1/14C12N3/00
Inventor 谭琳胡秋龙伍绍龙汤心砚杨中侠樊吉君
Owner HUNAN AGRICULTURAL UNIV
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