Composite microorganism additive agent for milk cattle feed stuff and method of preparing the same
A technology of microbial additives and compound microorganisms, applied in the field of microorganisms, can solve problems such as single efficacy, unsatisfactory effect, and unsatisfactory effects of dairy cow feeding
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[0040] 1. Materials and sources:
[0041] 1. The Bacillus subtilis strain in natto is the Bacillus subtilis (Bacillus subtilis) whose preservation number is CGMCC No.2420;
[0042] 2. The species of Enterococcus faecium is Enterococcus faecium with a preservation number of CGMCC No.2419;
[0043] 3. The strain of Saccharomyces cerevisiae is Saccharomyces cerevisiae with a preservation number of CGMCC No.2418;
[0044] 4. Source of soybean meal: China Animal Sunshine Feed Company.
[0045] 2. Preparation of compound microbial additives for dairy cattle feed
[0046] (1) Preparation of fermentation broth:
[0047] The preparation of natto bacillus subtilis bacterium liquid: get the two-ring activation bacillus subtilis natto bacterial classification from the PDA slant culture medium, its preservation number is CGMCC No.2420, connects and is equipped with 30mL seed culture medium (sucrose 1%, Soy peptone 1%, NaCl 0.5%, Na 2 HPO 4 0.6%, NaH 2 PO 4 0.1%, CaCl 2 0.02%, MgS...
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