A functional dairy cow feed additive for preventing recessive mastitis and reducing the number of somatic cells in dairy cows
A technology for recessive mastitis and dairy cow feed, which is applied in the field of dairy cow feed additives, can solve the problems of unsatisfactory animal feeding effect, failure to meet the needs of green and safety, etc., to improve biological utilization, ensure intestinal health, The effect of low moisture content
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Embodiment 1
[0023] Embodiment 1 Selenium-enriched yeast powder preparation
[0024] (1) Incline strains: Inoculate the strains of selenium-enriched yeast (Saccharomyces cerevisiae) CGMCCNo.0539 (purchased from the Institute of Microbiology, Chinese Academy of Sciences) on wort solids containing 15% sugar and 500 μg / ml sodium selenite concentration After culturing for 36 hours on the slant at 28°C, store in a 4°C refrigerator.
[0025] (2) First-level liquid strain culture: After activating the preserved selenium-enriched yeast strain, a ring of cells was cultured in 150 ml of wort with a total sugar content of 15% and a selenite concentration of 500 μg / ml Under the condition of 28° C., the rotation speed of the shaker is 180 rpm, and cultivated for 20 hours, which is the first-level liquid seed culture.
[0026] (3) Secondary liquid strain culture: Put the primary seed culture solution into a 3000ml Erlenmeyer flask of wort medium with a total sugar content of 15% and a selenite concentr...
Embodiment 2
[0031] Embodiment 2 Preparation of iron-rich yeast powder
[0032] (1) Incline strains: Inoculate iron-rich yeast (Saccharomyces cerevisiae) CGMCCNo.0895 (purchased from the Institute of Microbiology, Chinese Academy of Sciences) on wort solid slopes containing 8% sugar and 400 μg / ml ferrous sulfate After culturing for 48 hours at 28°C, store in a 4°C refrigerator.
[0033] (2) First-level liquid strain culture: After activating the preserved iron-rich yeast strains, connect a ring of cells to 150 ml of wort medium with a total sugar content of 10% and a ferrous sulfate concentration of 400 μg / ml In the Erlenmeyer flask, under the condition of 28 ℃, the rotation speed of the shaker is 180rpm, and cultivated for 18 hours, which is the first-level liquid seed culture.
[0034] (3) Secondary liquid strain culture: Put the primary seed culture solution into a 3000ml Erlenmeyer flask of wort medium with a total sugar content of 20% and a ferrous sulfate concentration of 600 μg / ml ...
Embodiment 3
[0039] Embodiment 3 Zinc-rich yeast powder preparation
[0040] (1) Incline strains: inoculate the strains of zinc-rich yeast (Saccharomyces cerevisiae) CGMCCNo.0896 (purchased from the Institute of Microbiology, Chinese Academy of Sciences) on the wort solid slope with sugar content of 10% and zinc sulfate concentration of 400 μg / ml , under the condition of 28°C, cultured for 48 hours, and stored in a 4°C refrigerator.
[0041] (2) First-level liquid strain culture: After activating the preserved zinc-rich yeast strains, connect a ring of cells to a medium containing 150 ml of wort medium with a total sugar content of 10% and a zinc sulfate concentration of 400 μg / ml. In the Erlenmeyer flask, under the condition of 28° C., the rotating speed of the shaker is 180 rpm, and cultured for 16 hours, which is the primary liquid seed culture.
[0042] (3) Secondary liquid strain culture: Put the first-level seed culture solution into a 3000ml Erlenmeyer flask of wort medium with a t...
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