199 results about "Sulforaphane" patented technology

Personal care composition comprising at least one hair growth regulating compound selected from the group consisting of glyceryl dilaurate, apigenin, tetrahydrocurcumin, oleanolic acid, azelaic acid, sulforaphane, canavanine, pyridoxal 5-phosphate, phytic acid, tannic acid, grape seed extract, NG-nitro-L-arginine-methyl ester, benzamidine, sodium butyrate, betulinic acid, polyornithine, polyarginine, fisetin, jasmonates, methyl-jasmonate, cis-jasmone, caffeic acid phenethyl ester, delphinidin, ethyl abietate, esculetin, sorbic acid methyl ester, canaline, N-formyl-methionine, N-formyl-alanine, taurine, palmitoyl carnitine, undecanol, undecylenic acid, rutin, fusidic acid, phenyl pyruvic acid, L-isoleucine, phenyl glycine, silibinin, silymarin, L-ascorbic acid-6-palmitate, N-undecylenoyl-L-phenylalanine, and salts, derivatives and mixtures of any of the foregoing; and a dermatologically-acceptable carrier.

Disclosed herein are methods and uses for preventing melanoma, reducing progression of atypical nevi, and inducing cell cycle arrest and / or apoptosis in a melanoma cell through oral and enteral administration of sulforaphane to subjects indicated to be at risk due to factor(s) such as medical history of atypical nevi, melanoma, or UV exposure. Sulforaphane can be administered orally as a safe and well-tolerated natural agent as a chemopreventive strategy in individuals with atypical melanocytic nevi.

The invention relates to a process for preparing brassica vegetable seedling powder chewable tablets and a brassica vegetable seedling powder chewable tablet product, belonging to the technical field of deep processing of agricultural products. The process is characterized by comprising the following steps of: with brassica vegetable seedling powder, such as broccoli and the like as a main basic stock, compounding, preparing a soft material, granulating, drying, tabletting, sterilizing and packing to prepare the brassica vegetable seedling powder chewable tablet with the advantages of clean appearance, green color and crispness. The process for preparing the brassica vegetable seedling powder chewable tablet, disclosed by the invention, is simple, has high industrial degree and can realize complete utilization on the brassica vegetable seedling, such as broccoli and the like, thereby increasing the additional value of the brassica vegetable seedling. The brassica vegetable seedling powder chewable tablets, including the broccoli and the like, prepared by the process disclosed by the invention have functions of resisting oxidation, slowing aging and resisting inflammation and cancer and the like of human bodies, and is a kind of casual healthcare food. According to the product of the process for preparing the brassica vegetable seedling powder chewable tablets, disclosed by the invention, the content of 4-methylsulfinylglucosinolate is up to 185.0-413.0mg / 100g, and the content of sulforaphane provided by each chewable tablet is equal to that provided by 30-75g of fresh brassica vegetable, such as broccoli and the like.

A method of stabilizing sulforaphane is provided. The method includes contacting sulforaphane, or an analog thereof, and a cyclodextrin to form a complex between the sulforaphane, or analog thereof, and the cyclodextrin.

The invention discloses a sulforaphane microencapsulation method which comprises the following steps of: I, extraction of sulforaphane: performing digestion of the raw material crucifer to extract the sulforaphane, wherein the extraction concretely comprises the following steps of: A, grinding seeds; B, adding petroleum ether for degreasing; C, adopting phosphate buffer solution with pH value of 7.0; D, performing enzymolysis; E, adding an extractant for extraction; F, adding sodium sulfate and sodium chloride respectively, and filtering; G, performing vacuum concentration to remove the organic solvent; H, fixing the volume to 5,000ml with methanol; I, filtering with a 0.22-micron microfiltration membrane; and J, measuring the content of sulforaphane by liquid chromatography; and II, microencapsulation of sulforaphane: adding monoglyceride and sodium alginate into the composite wall material, adding the deionized aqueous solution, adding the sulforaphane while stirring, spray-drying and collecting the product. In the method disclosed by the invention, the extremely unstable sulforaphane liquid is synthesized into powdery solid particles through a microencapsulation method so as to obtain perfect stability.

The invention discloses a method for extracting high-purity sulforaphane from broccoli seeds; and the method comprises the following steps of: (1) after crushing dry broccoli seeds, adding water to the dry broccoli seeds to hydrolyze the broccoli seeds, so as to obtain a solid-liquid mixture; (2) adding anhydrous ethanol to the mixture; ultrasonically extracting the mixture; and carrying out suction filtration or double-layer gauze filtering on the mixture; (3) concentrating and evaporating ethanol from a filter liquor in a vacuum state; (4) carrying out chromatography on a material liquor by using a macroporous resin column and eluting the material liquor by using an ethanol solution in a gradient way; (5) collecting an eluting solution containing sulforaphane; carrying out chromatography on the eluting solution by using an alumina column; and concentrating a sulforaphane chromatography solution to form an extract so as to obtain a crude sulforaphane product; (6) adding water to the obtained crude product so as to dissolve the crude product; and extracting the dissolved crude product by using a polar solvent; and (7) collecting an organic layer; and concentrating the organic layer in a vacuum state so as to obtain an oily extract, so that the high-purity sulforaphane is obtained. As the high-purity sulforaphane can be directly extracted from the broccoli seeds and broccoli related materials, the method for extracting the high-purity sulforaphane from the broccoli seeds, disclosed by the invention, has the advantages of simple process, stable product, simple equipment and safety in production and is suitable for enterprises for industrially extracting the sulforaphane.

The invention provides a synthetic method for sulforaphane and belongs to the field of drug synthesis. The method comprises the following steps that: after amino in 4-amino-1-butanol is protected by Boc groups, hydroxy in 4-amino-1-butanol is changed into methanesulfonyl ester by methanesulfonyl chloride, and then the resultant reacts with sodium methyl mercaptide to produce 4-methylthio butyl-1-tert-butoxycarbonylamide; Boc protective groups are removed under acidic condition to obtain 4-methylthio-1-butylamine; 4-methylthio-1-butylamine reacts with carbon disulfide for one hour in the presence of triethylamine and p-toluenesulfonyl chloride is added for treatment for half an hour to produce 4-methylthio butyl-1-isothiocyanate; and at last 4-methylthio butyl-1-isothiocyanate is oxidized by m-CPBA to produce sulforaphane. According to the invention, complex hydrazinolysis of phthalimide in aftertreatment is avoided and toxic thiophosgene is not needed in the preparation of isothiocyanate; overall yield of sulforaphane is 64%, substantially higher than the overall yield of 8% reported in literature; the whole preparation process is simple and time-saving and is suitable for large scale production.

The invention relates to a method for extracting sulforaphane from broccoli. The method comprises the following steps: (1) drying edible flowering branches of fresh broccoli to constant weight and crushing to obtain broccoli dry powder; (2) carrying out vacuum filtration on newly harvested crushed mustard seeds to remove impurities to obtain a crude enzyme; (3) adding the crude enzyme into the broccoli dry powder, uniformly stirring, and carrying out enzymolysis and ultrasonic vibration to extract to obtain extracting liquor which is screened and deslagged to obtain a filtrate; carrying out vacuum filtration on the filtrate to obtain sulforaphane coarse extracting liquor; (4) vacuum concentrating the sulforaphane coarse extracting liquor to obtain sulforaphane coarse extracting aqueous liquor; (5) adding ethyl acetate into the sulforaphane coarse extracting aqueous liquor to extract to obtain an ethyl acetate phase, carrying out vacuum concentration on the ethyl acetate phase to get paste, and then eluting the paste and further vacuum-concentrating to obtain a paste sulforaphane coarse extract; (6) freezing and drying the paste sulforaphane coarse extract to constant weight to obtain the sulforaphane coarse product which is 1-5% in purity. The method provided by the invention is simple, efficient and low in cost.

The invention discloses a seed extract of Broccoli and relevant preparation method. The preparation method is to receive the extract of the Broccoli seed hydrolyzing, degreasing, inactivating, and condensing the seed of the Broccoli and to receive a health product by adding auxiliary materials. The main components of the Broccoli extract of the invention is compounds of isothiocyanate; wherein, sulforaphane is the most activated compound with maximal content, and sulforaphane is also the isothiocyanate compound with maximal cancer-prevention activity. The health product containing the Broccoli extracts can effectively help human body improve the resistance to toxicants after the proof of animal experiment, thereby effectively reducing the formation of tumor induced by chemical matters.

The invention relates to the technical field of medicines, and provides a docetaxel and sulforaphane loaded hyaluronic acid modified poly(lactic-co-glycolic acid) copolymer self-assembled nano-particle, a preparation method thereof and an application of the self-assembled nano-particle in preparing a medicine for treating breast cancer. The microscopic structure of the nano-particle comprises a hydrophobic PLGA (poly(lactic-co-glycolic acid)) core and a hydrophilic HA (hyaluronic acid) shell, wherein the PLGA core is used for wrapping a medicine DTX (docetaxel) or SFN (sulforaphane), and the hydrophilic shell HA can target a breast cancer stem cell highly expressing a CD44 receptor. In-vitro cytotoxicity tests indicate that the drug-loaded nano-particle can play a good role in resisting differentiation of mammary gland cells and breast cancer stem cells when compared with free medicines; in-vivo tumor inhibition experiments indicate that the drug-loaded medicine is more effective than free medicines and combined free medicines, and has a small systematic toxicity. The nano-particle can play a role in simultaneously performing target differentiating on mammary gland cells and breast cancer stem cells, and a new strategy is provided to treatment of breast cancer.

The invention provides a method for preparing high-purity sulforaphane and in particular relates to a method for extracting the sulforaphane from radish seeds. The method is used for preparing products having the purity of more than 98% from air-dried radish seeds as the raw material and through the steps of cracking, degreasing, hydrolysis, initial separation by use of resin, reverse osmosis concentration, preparation separation and freeze-drying. The method is simple and convenient to operate, simple in equipment, mild in reaction conditions, energy-saving, low in production cost, and convenient to popularize and utilize. The used methanol waste liquid is recovered, the used resin is regenerated and impurities generated can be recycled, and therefore, the production is safe, green and pollution-free, and the production method is capable of saving energy and reducing emission. The sulforaphane product prepared by the method can be widely applied to the industries such as medicines and health care products.

The invention relates to a method for extracting sulforaphane from leaves of cauliflowers, which comprises the following steps of: after bulbs of the cauliflowers are harvested, immediately collecting waste leaves, cleaning, and performing freezing treatment in an ultralow temperature refrigerator; performing vacuum freeze drying, uniformly crushing into powder, taking 1 part of powder, adding exogenous myrosinase in an amount which is 6 to 10 times weight of the powder and deionized water in an amount which is 4 to 6 times weight of the powder, and fully mixing into uniform slurry; and performing enzymolysis reaction, solvent extraction, filtration, concentration, and vacuum freeze drying to obtain high-purity sulforaphane. The raw material sources are rich, the extraction method is simple, and the method is more suitable for industrial production.

The invention relates to a method for enriching and extracting sulforaphane from brassica vegetable seeds and belongs to the technical field of food biological technology. The method is characterized by comprising the following steps of: taking the sulforaphane brassica vegetable seeds with germinating ability as raw materials; crushing the raw materials and adding a Tris-HCl buffer solution according to the ratio of the materials to liquid being (w / v) 1:(3-5); adjusting the pH value to 6.0-8.0 to form an incubation buffer; adding vitamin C and Zn<2+>, activating endogenous myrosinase in the vegetable seeds and hydrolyzing glucosinolate to form the sulforaphane with an anti-cancer effect; and extracting the sulforaphane from the incubation buffer with ethyl acetate and carrying out filtering, vacuum concentration, ultrasonic extraction and vacuum condensation to obtain a sulforaphane product. The method disclosed by the invention has the characteristics of wide application range of the raw material, low production cost, high enrichment quantity of the sulforaphane and the like. The method is suitable for health-care food sulforaphane products.

The invention discloses a chemical synthesis method of sulforaphane. The method is characterized by comprising the following steps of: (a) in the presence of a catalyst, heating 1,3-propylene glycol and hydrobromic acid for reacting to obtain 3-bromine-1-propyl alcohol; (b) reacting dimethyl sulfoxide and sodium hydride at normal temperature, after the dimethyl sulfoxide and the sodium hydride completely react, adding the 3-bromine-1-propyl alcohol, and stirring; after the 3-bromine-1-propyl alcohol completely reacts, washing with a small quantity of water; extracting by using dichloromethane, taking an organic layer, and carrying out spin evaporating on the organic layer to obtain white solid 4-methylsulfonyl-1-butanol; (c) dissolving the 4-methylsulfonyl-1-butanol in an organic solvent, adding a chlorinated reagent for a chlorinated reaction to obtain 1-chlorine-4-methylsulfonyl butane; and (d) under the protection of nitrogen, dissolving the 1-chlorine-4-methylsulfonyl butane in an organic solvent, adding a thiocyanide reagent and heating, after the reaction ends, washing with water, extracting by using an organic solvent, and carrying out spin evaporating to remove the solvent to obtain faint yellow oily liquid, i.e. the sulforaphane. The method has the advantages of simpleness of operation, high yield and stable product.

The invention belongs to the field of natural product separation and discloses a method for preparing sulforaphane, which comprises the following steps of: 1) crushing a radish seed medicinal material, adding water in an amount which is 2 to 3 times that of the crushed medicinal material, regulating the pH to be 4-5, performing enzymolysis at the temperature of between 45 and 50DEG C for 30 to 60 minutes, quickly heating to 80DEG C for deactivating enzymes, adding water in an amount of 5 to 6 times that of the solution, stirring, extracting, filtering out liquid, adding water in an amount which is 5 to 6 times that of the materials, extracting, mixing liquid in two times, and filtering to obtain extracting solution; 2) adsorbing the extracting solution by using macroporous resin, washing with water to reach neutrality, eluting by using 50- to 70 percent of ethanol solution in an amount which is 5 to 6 times that of the solution, collecting eluent, performing nanofiltration by using a nanofiltration membrane, and concentrating the passing liquid by using a reverse osmosis membrane to obtain concentrated solution; and 3) extracting the concentrated solution by using dichloromethane, and recovering reagents from extracting solution under reduced pressure to obtain the sulforaphane. The sulforaphane is prepared by using the macroporous resin and a membrane separation technology; and the process is easy to operate, the production cost is low and the method is suitable for industrial production.

This invention relates to compositions comprising zinc and sulforaphane which can be used in the treatment or prevention of cancer. The invention also relates to methods of treating or preventing cancer, including prostate cancer, which comprises the administration of zinc and sulforaphane to a patient in need thereof.

The present invention relates to methods of isolating and purifying sulforaphane. More specifically, the present invention relates to methods of isolating and purifying sulforaphane from natural sources. The present invention also relates to methods of forming high purity complexes of sulforaphane with cyclodextrin.

The present invention is directed to chemoprotectant precursor compositions provided from crucifer seeds and sprouts and methods for their preparation. Treatment of aqueous extracts from crucifer seeds or sprouts with adsorbents substantially increases the ratio of certain highly chemoprotectant precursor compounds (alkyl glucosinolates such as glucoraphanin, a.k.a. sulforaphane glucosinolate) to undesirable compounds such as indole glucosinolates (for example 4-hydroxyglucobrassicin). The method provides an extract which has a ratio of glucoraphanin to 4-hydroxyglucobrassicin of about 70 or greater. The resulting extract has improved color and odor and may be dried or used directly as an additive in a variety of foodstuffs.

The invention discloses a new preparation method of 1-isothiocyanic acid-4-methanesulfonyl-(2-ene) butane, and particularly relates to a method for the extraction of sulforaphane from radish seeds or broccoli seeds or rape seeds. According to the method, air-dried radish seeds or broccoli seeds or rape seeds are used as a raw material for preparation of a radish seed crushing liquid, an enzyme deactivated broccoli seed or radish seed dispersion liquid, a degreased fine grinding liquid, a fermentation liquid, a protein removal fermentation liquid, a concentrated liquid, dry polysaccharide powder and high purity sulforaphane freeze-dried powder for preparation of 1-isothiocyanic acid-4-methanesulfonyl-(2-ene) butane with the purity of more than 98%. The production rate of the sulforaphane prepared by the method is high, the operation is simple, and the product has the characteristics of being high in product purity of more than 98%, green, non-pollution and the like. The product prepared by the method can be widely used in medicine and health care industry, and has cancer preventing and resistant function.

The anti-cancer combined preparation containing sulforaphane and platinum drugs is used for the treatment of cancer, especially lung cancer, and the components can be administered simultaneously, separately or sequentially. Among them, sulforaphane increases the anticancer effect of platinum anticancer drugs.

The invention discloses a fertilizer capable of improving the content of sulforaphane in cultivated vegetable and an application method thereof. The fertilizer contents 30-150ppm of nitrogen, 8-128ppm of sulfur and 25-200ppm of methyl jasmonate. The method for improving the content of sulforaphane in the cultivated vegetable by using the fertilizer is as follow: the fertilizer is applied to vegetable plants. The fertilizer and the method provided by the invention can be used for solving the problem that the content of sulforaphane is low and have wide application prospects.

The invention relates to a preparation method of a sulforaphane capsule. The method comprises the following steps: (1) dehydrating, drying and smashing a flowering branch of an edible part of fresh broccoli to obtain broccoli dry powder; (2) smashing new harvested mustard seeds, adding distilled water, and carrying out ultrasonic oscillation and suction filtration to obtain crude enzyme; (3) uniformly mixing the broccoli dry powder with the crude enzyme, carrying out enzymolysis and drying to obtain dry broccoli dry powder; (4) adding ethyl alcohol to the broccoli dry powder, carrying out ultrasonic oscillation extraction, sieving and suction filtration to obtain sulforaphane crude extract; (5) concentrating the sulforaphane crude extract to obtain sulforaphane aqueous solution; (6) diluting the sulforaphane aqueous solution to obtain the sulforaphane aqueous solution with the sulforaphane content of 3-5mg / mL; (7) adding the sulforaphane aqueous solution to the dry broccoli powder, and drying after uniform mixing to obtain redried broccoli powder; (8) smashing the redried broccoli powder for capsule filling to obtain the sulforaphane capsule with the sulforaphane content of 6-14mg / g. The preparation method is simple to operate and low in cost.

The invention relates to a compound metagen solid beverage and a preparation method thereof. The solid beverage is prepared from the following components in percentage by mass: 15-20% of sulforaphane, 20-25% of sodium butyrate, 25-30% of metagen solid powder, 18-22% of an adhesive, 4.5-6.0% of a sweetening agent and 3.5-6.0% of a sour agent, wherein the metagen solid powder comprises lactobacillus paracasei G15 and Q14 powder, lactobacillus rhamnosus powder and bifidobacterium powder; and the four kinds of lactobacillus powder are obtained by drying supernatants of respective bacilli. The metagen solid beverage is especially suitable for crowds with special requirements, such as crowds with hyperlipidemia, crowds with fatty liver and crowds with weight loss.

Disclosed is a method for preparing broccoli with increased sulforaphane content using high-voltage pulsed electric field treatment. According to the method, broccoli with increased sulforaphane content can be eaten raw or can be chopped to appropriate sizes before eating without the need to crush the broccoli. In addition, sulforaphane can be extracted from the broccoli with increased sulforaphane content. The broccoli with increased sulforaphane content and the sulforaphane extract produced therefrom can be used as an active ingredient of a food, feed or cosmetic composition to exhibit the activities of sulforaphane, including antioxidative and anti-inflammatory activities.

The invention relates to a method for extracting high-purity sulforaphane by taking broccoli seeds as a raw material. The method comprises the steps of degreasing the crushed broccoli seeds by using normal hexane, filtering, adjusting the pH value of a water solution to 4.5-5.5, carrying out enzymolysis, extracting dichloromethane, and finally carrying out column chromatography on silica gel, purification and the like to obtain a final product. The method is high in extraction rate and product purify. The method has the advantages of being high in extraction rate, simple in process, stable in product quality, easy in industrial production, etc.

The invention provides an anti-tumor and immune-modulating composition, a preparation method and an application of the composition in preparing anti-tumor and immune-modulating medicines. The preparation composition is formed by mixing 1 to 20 parts of broccoli extracting solution, 0.5 to 1 part of chitosan oligosaccharide, 1 to 2 parts of ganoderan, 1 to 2 parts of cordyceps polysaccharide and 1 to 2 parts of dextrin in parts by weight; the preparation composition is characterized in that the content of sulforaphane is 2 to 15 mg / g and the content of polysaccharide is 200 to 400 mg / g in the preparation composition; the preparation method comprises the following steps: crushing, porphyrizing, filtering, hydrolyzing and macroporous resin adsorption separating broccoli seeds or corms to obtain a water solution containing sulforaphane, uniformly mixing the broccoli extracting solution, chitosan oligosaccharide, ganoderan, cordyceps polysaccharide and dextrin with water, spray drying into fine powder, preparing into granules, tablets, capsules and the like. Animals researches prove that the compound polysaccharide preparation has remarkable effects on improving immunity and suppressing tumors.

The invention discloses a cultivation method for broccoli buds rich in sulforaphane. The cultivation method is characterized by comprising the steps of the extraction of sedum aizoon crude flavonoids, the preparation of a self-made culture solution, the selection and processing of broccoli seeds, the germination of the broccoli seeds, the reveal of the broccoli seeds, the cultivation of the broccoli seeds through using a mode that the revealed broccoli seeds are placed on gauze which is wetted by the self-made culture solution under a certain light condition and an environment with the temperature of 20-25 DEG C and the humidity of 80-90 %, the spraying of the self-made culture solution every other 24 hours, and the harvesting when the broccoli buds grow to 8-10 mm or the broccoli roots are 10-12 mm long to obtain the broccoli buds rich in the sulforaphane. The cultivation method has the advantages that the resistance to corrosion is strong, the yield is high, the content of the sulforaphane is high, the cultivation method is simple, and the effect is rapid.

The invention discloses a method for extracting and purifying sulforaphane from broccoli seeds. The method comprises the following steps: 1, crushing broccoli seeds, degreasing the crushed broccoli seeds by using a low-polarity reagent in order to remove grease in the broccoli seeds, and drying the degreased broccoli seeds to obtain dry broccoli seed powder; 2, adding water and a pH value adjusting agent to the degreased and dried dry broccoli seed powder to obtain an acidic solution, and hydrolyzing the dry broccoli seed powder to obtain a hydrolysate; 3, adding water to the hydrolysate, carrying out ultrasonic extraction, and filtering the above obtained solution in order to obtain a sulforaphane extract liquid; and 4, adding a water saturated regent to the sulforaphane extract liquid to carry out extraction, collecting the obtained organic phase, and concentrating the organic phase to form an oily product in order to obtain oily sulforaphane. The method has the advantages of simple technology, low cost, good selectivity and the like, overcomes the preparation scale restriction of small batch and laboratories, and is in favor of the industrial production of sulforaphane.