107 results about "Sulforaphane" patented technology

The invention relates to a bifidobacterium animalis subsp. Lactis B7 as well as a composition and application thereof, and belongs to the technical field of microorganisms, the bifidobacterium animalis subsp. Lactis B7 is preserved in the China Center for Type Culture Collection on April 19, 2024, and the preservation number is CCTCC NO: M2024734. The bifidobacterium animalis subsp. Lactis B7 is named as Bifidobacterium animalis subsp. Lactis B7, the composition is named as Bifidobacterium animalis subsp. Lactis B7, and the composition is named as Bifidobacterium animalis subsp. Lactis B7. The invention also discloses application of the B7 fermentation composition in preparation of medicines for preventing and treating ulcerative colitis, constipation and constipation-type ulcerative colitis. According to the bifidobacterium animalis subsp. Lactis B7, high-yield short-chain fatty acid and indolepropionic acid are achieved, sulforaphane is generated through glucoraphanin, intestinal tract movement is improved, ulcerative colitis or inflammatory bowel disease activity index DAI and weight loss caused by the disease activity index DAI are effectively relieved, mouse colon injury is remarkably relieved, and mucosa layers are protected; the effects of resisting inflammation, inhibiting pathogenic bacteria and improving the intestinal barrier function are achieved.

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention discloses application of BoSLIM1 protein in increasing the content of sulforaphane and the content of indole-3-methanol in broccoli, and belongs to the technical field of plant breeding. The invention aims to improve the content of sulforaphane and indole-3-methanol in broccoli at the same time. The invention provides an application of a protein shown as SEQ ID NO.2 in increasing the content of sulforaphane or / and the content of indole-3-methanol or the content of glucoraphanin or / and the content of brassica glucosinolate in broccoli. The content of sulforaphane and indole-3-carbinol in the broccoli is increased at the same time, the nutritional value of the broccoli is greatly increased, the requirements of modern consumers are met, and people can obtain nutritional ingredients in vegetables easily, so that the broccoli is beneficial to body health.

The present disclosure relates to novel small molecules, particularly, sulforaphane analogues (SFNAs) as well as αCD / SFNAs inclusion complexes and their use as lead compounds for manufacturing a medicament or a pharmaceutical composition for therapy, in particular, for treating cancer.

The invention provides a BoNIA2b gene, which has a nucleotide sequence as shown in SEQ ID No.1, or a nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function, wherein the nucleotide sequence is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1; the invention also provides an application thereof. Compared with the prior art, the broccoli BoNIA2b gene has the following advantages that functional verification is carried out on the broccoli BoNIA2b gene, it is found that overexpression of the BoNIA2b gene can promote increase of the content of glucoraphanin and sulforaphane in the hairy roots of broccoli, and it is found through further research that the content of glucoraphanin and sulforaphane in the hairy roots of broccoli can be increased; the overexpressed BoNIA2b gene is mainly used for regulating and controlling the expression level of some synthetic genes of glucoraphanin and sulforaphanin of the hairy roots of broccoli so as to regulate and control the synthesis of glucoraphanin and sulforaphanin. Finally, the BoNIA2b gene is proved to positively regulate the synthesis reaction of glucoraphanin and sulforaphane of broccoli.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

This paper discusses a strain of Bifidobacterium animalis subsp. lactis B7 and its composition and applications, belonging to the field of microbial technology. The specific strain is Bifidobacterium animalis subsp. lactis B7. Bifidobacterium animalis subsp. lactis Bifidobacterium lactis subspecies B7 was deposited at the China Center for Type Culture Collection on April 19, 2024, with accession number CCTCC NO: M2024734. This invention also discloses the application of the B7 fermentation composition in the preparation of drugs for the prevention and treatment of ulcerative colitis, constipation, and constipation-predominant ulcerative colitis. This animal bifidobacterium lactis subspecies B7 produces high levels of short-chain fatty acids and indolepropionic acid, utilizes sulforaphane to generate sulforaphane, increases intestinal peristalsis, effectively alleviates the disease activity index (DAI) and weight loss caused by ulcerative colitis or inflammatory bowel disease, significantly reduces colonic damage in mice, protects the mucosal layer, has anti-inflammatory effects, inhibits pathogens, and improves intestinal barrier function.

The present invention relates to sulforaphane glycoconjugate compounds, as well as to the synthesis and therapeutic uses thereof for treating inflammatory diseases.

The invention relates to the technical field of traditional Chinese medicine processing, and discloses a radish cream extraction method, which at least comprises: S1, respectively crushing radish and mirabilite, adding water, and uniformly mixing to obtain a mixture; s2, placing the mixture obtained in the step S1 in an environment with the temperature of 0-20 DEG C and the relative humidity of 1-60%; and S3, after the frost is separated out in the S2, collecting until the frost is separated out. According to the invention, the process for preparing the radish cream from the mirabilite is researched for the first time, and a basis is provided for production and clinical research significance of glucosinolate and sulforaphane in radishes and establishment of quality standards of the radish cream. The main component of the radish cream is recrystallized Na2SO4. 10H2O, and the result is obtained through experimental verification. The radishes and the mirabilite have a synergistic effect, so that the medicine is purer, and the effects of relieving swelling and pain and clearing away heat and toxic materials are enhanced. The radish cream is used for treating sore throat, mouth and tongue sores, swelling and aching of gum, acute and chronic pharyngolaryngitis, tonsillitis, stomatitis and oral ulcer symptoms.

The invention relates to the combination of a sulforaphane precursor, an enzyme capable of converting the sulforaphane precursor to sulforaphane, an enzyme potentiator, and a milk thistle extract or powder. The invention also relates to the combination of a sulforaphane or a derivative thereof and a milk thistle extract or powder. The invention also relates to the combination of a broccoli extract or powder and a milk thistle extract or powder. The invention provides compositions and methods relating to these combinations.

The present invention discloses the application of sulforaphane in the preparation of a product for treating aplastic anemia, and belongs to the field of biotechnology. The present invention finds that sulforaphane (SPN) can not only reverse the disease degree of aplastic anemia, but also achieve the purpose of treating AA by inhibiting the occurrence of ferroptosis. Therefore, the use of sulforaphane in the preparation of a product for preventing and treating aplastic anemia must have a good application prospect, and the present invention provides a new solution for the treatment of aplastic anemia.

A composition including a Moringa plant component for treating, preventing, reducing the occurrence of, decreasing the symptoms associated with, and / or reducing secondary recurrences of cancer in a subject is provided. The composition may comprise a Moringa plant component in an amount of 1 mg to 1750 mg, and sulforaphane or a sulforaphane derivative in an amount of 1 mg to 50 mg. Alternatively, the composition may comprise a Moringa plant component in an amount of 150 mg to 1500 mg sulforaphane precursor in an amount of 1 mg to 50 mg. The Moringa plant component can be Moringa leaf extract containing 10 mg of moringin per 1000 mg of the Moringa leaf extract. The composition may also include at least one of a mushroom extract or powder and a broccoli extract or powder.

The invention discloses application of sulforaphane, and belongs to the field of medicine. Experiments with mouse AML-12 cells find that when the mouse AML-12 cells are jointly treated with sulforaphane and alpha-amatoxin, the inhibition effect of alpha-amatoxin on the activity of the AML-12 cells can be effectively relieved, the ROS level in the cells can be reduced, cell oxidative stress injury can be relieved, mitochondrial membrane potential reduction can be inhibited, and the mouse AML-12 cells can be treated with raphane and alpha-amatoxin. The sulforaphane can be used for relieving alpha-amatoxin induced mouse AML-12 cell mitochondrial injury, so that the sulforaphane can become a novel medicine for relieving alpha-amatoxin poisoning. The new application of the sulforaphane is found for the first time, and a new substance is provided for preparing the medicine for relieving alpha-amatoxin poisoning.

The invention discloses application of glucoraphanin to preparation of a product for reducing biological accumulation of perfluorooctane sulfonate, and belongs to the technical field of biological medicine. The occurrence probability of the high PFOS level in the body of a participant frequently eating broccoli is confirmed to be remarkably reduced through population epidemiological experiments, and then the PFOS level in the body of the participant is confirmed to be remarkably reduced after glucoraphanin intervention through clinical RCT random control experiments. The glucoraphanin-intervened serum has the advantages that the glucoraphanin-intervened serum is used as a main body, the metabolic change of steroid, ceramide and bile acid in the glucoraphanin-intervened serum is confirmed to be closely related to the reduction of the PFOS level through lipidomics analysis, and finally, the glucoraphanin is confirmed to be capable of effectively reducing the PFOS in-vivo accumulation and improving the liver injury caused by the PFOS through animal experiments and an AI reverse targeting technology; and the glucoraphanin and the in-vivo metabolite sulforaphane thereof have relatively strong binding capacity with a liver-specific PXR receptor, so that a good technical foundation is laid for research and development of drugs for treating diseases related to PFOS accumulation.

To provide compositions for increasing the level of NAD(P)H:quinone oxidoreductase 1 (NQO-1), or increasing its gene expression.SOLUTION: Provided is a composition for increasing the level of NAD(P)H:quinone oxidoreductase 1 (NQO-1) or increasing its gene expression in a subject. The composition comprises a combination of sulforaphane and a glucan as active ingredients, which combine to produce a synergistic effect between the active ingredients. The glucan is provided as a Ganoderma lucidum extract or powder. The Ganoderma lucidum extract preferably contains about 1 to about 75 wt.% of one or more glucans. Furthermore, the one or more glucans are preferably β-glucan and / or 1,3-β-glucan and / or 1,6-β-glucan.SELECTED DRAWING: Figure 1

The present invention is characterized by being a novel sulforaphane derivative comprising a complex of sulforaphane (1-isothiocyanato-4-[methylsulfinyl]butane (SFN)) and cysteine persulfide (CysSnH, n=2, 3, 4, or 5) or glutathione persulfide (GSnH or GSnG, n=2, 3, 4, or 5).

The utility model relates to the technical field of chemical substance extraction, in particular to sulforaphane extraction equipment, which comprises a crushing bin and an extraction bin, a blanking hopper is arranged at the top of the crushing bin, and the top and the bottom of the crushing bin are respectively communicated with the bottom of the blanking hopper and the top of the extraction bin; an affiliated table is arranged on the right side of the extraction bin, a water tank is arranged at the left end of the top surface of the affiliated table, and the bottom of the water tank is detachably connected with the affiliated table; a support is arranged at the right end of the top face of the affiliated table, and the bottom of the support is fixedly connected with the affiliated table through screws. According to the sulforaphane extraction equipment, a water leaching extraction method is adopted, a heating rod and an acid-base adjusting solvent are additionally arranged to ensure that the temperature and the pH value of water are in the optimal numerical values for forming sulforaphane through hydrolysis, and a heating plate is additionally arranged in the hydrolysis bin, so that a constant-temperature state is ensured within the extraction time, and the influence of temperature change on extraction is prevented.

The invention discloses a chitosan modified turmeric-broccoli exosome hybrid nasal spray and application thereof, and belongs to the technical field of biological medicine and drug delivery. The nasal spray is formed by dispersing double plant exosome-lipidosome hybrids in a carrier for nasal cavities. The preparation method comprises the following steps: fusing curcumin carried by a turmeric exosome and sulforaphane carried by a broccoli bud exosome with a pH response liposome membrane containing 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine, and modifying by chitosan to form a three-layer structure; the preparation method comprises the steps of exosome extraction, ultrasonic drug loading, liposome hybridization, chitosan coating and preparation blending. The nasal spray is high in entrapment efficiency and uniform in particle size, the chitosan enhances nasal adhesion, inflammation targeted drug release is achieved through pH response, the brain targeting efficiency and stability are excellent, and the adaptability to children is good. The curcumin and sulforaphane are used for targeted therapy of children autism spectrum disorder neuroinflammation, anti-inflammation and anti-oxidation are achieved through synergism of curcumin and sulforaphane, the curative effect is remarkable, and a new scheme is provided for treatment of related diseases.

This invention belongs to the field of biomedical technology and relates to the application of OSM inhibitors in the preparation of drugs for the prevention or treatment of calcific aortic valve disease. This invention reveals for the first time that OSM drives osteogenic differentiation and calcified nodule formation of valvular interstitial cells through the OSM-OSMR-JAK2 / JAK3-STAT3-RUNX2 signaling axis, which is the core pathogenic mechanism leading to calcific aortic valve disease. This invention utilizes anti-OSM neutralizing antibodies or sulforaphane to effectively block this signaling axis, significantly inhibiting macrophage infiltration, valvular calcification, and improving hemodynamic abnormalities. This invention provides a precise target and a novel strategy for the modification therapy of calcific aortic valve disease, solving the technical problem of the ineffectiveness of traditional broad-spectrum anti-inflammatory therapy in valvular calcification.

The present invention relates to the technical field of antioxidant technology for livestock sperm, and provides a highly efficient antioxidant donkey sperm diluent, a preparation method thereof, and an application. A highly efficient antioxidant donkey sperm diluent includes a basal medium and additives, wherein: the basal medium contains the following components by weight in every 1000 mL of water: 30 - 60 g of glucose, 4 - 6 g of 4 - (2 - hydroxyethyl)piperazine - 1 - ethanesulfonic acid, 12 - 24 g of skim milk powder, and 0.5 - 1.1 g of potassium citrate; the additives contain the following components by weight in every 1000 mL of the basal medium: 5 mg - 10 mg of sulforaphane, 0.1 mg - 0.2 mg of stigmasterol, 40 μg - 100 μg of enrofloxacin concentrate, and 3 mg - 5 mg of cefotaxime sodium. The present invention can effectively improve the conception rate of female donkeys, reduce the incidence of metritis, and improve the in vitro sperm motility.

This invention discloses a composition for female ovarian maintenance and its preparation method, relating to the field of health care compositions, including the following steps: mixing pectin and sweetener, dissolving in water to obtain material A; then mixing the traditional Chinese medicine formula, material A, active ingredients, resistant dextrin, vitamins, flavoring agents, and edible flavorings in sequence; stirring after adjusting the volume with purified water, then filtering, filling, sterilizing, and packaging to obtain a composition for female ovarian maintenance; this invention improves the utilization rate of functional components through the immobilization of fructooligosaccharides and β-glucosidase and the enteric coating targeting of shellac; Poria cocos polysaccharide-sulforaphane-resveratrol covers immune, antioxidant, and metabolic regulation pathways, directly targeting the core mechanism of premature ovarian failure; in summary, through the optimization of the entire chain of delivery-conversion-absorption-metabolism, the efficacy is significantly improved under the premise of safety.

This invention relates to broccoli rich in sulforaphane and indole-3-methanol, its preparation method, and its applications, belonging to the field of food technology. To increase the content of sulforaphane and indole-3-methanol in broccoli, this invention provides a broccoli rich in sulforaphane and indole-3-methanol and its preparation method. This invention utilizes expression vectors or recombinant bacteria overexpressing the BoSULTR1;1 and BoSULTR1;2 genes to prepare transgenic broccoli plants. Nutrient content determination results of the above transgenic broccoli plants show that the BoSULTR1;1 and BoSULTR1;2 genes increase the content of sulforaphane and indole-3-methanol in broccoli by increasing the content of the precursor substances sulforaphane and brassinosteroides. This invention achieves the goal of simultaneously increasing the content of sulforaphane and indole-3-methanol in broccoli, significantly improving the nutritional value of broccoli, and can be applied to the production of foods beneficial to health.

The present invention discloses a method for extracting natural sulforaphane and its application in antibacterial gel, belonging to the technical field of plant extracts. The present invention utilizes the enzymatic hydrolysis of a homemade exogenous mustard seed enzyme solution, combined with ultrasound and microwave assistance, and then combined with ethyl acetate extraction and resin separation and purification processes, to finally extract sulforaphane from broccoli seeds. After testing, the purity of the extracted natural sulforaphane is 87.5% to 88.2%. Then, the present invention utilizes the extracted sulforaphane to prepare an antibacterial gel, which is composed of a gel, an auxiliary agent glycerol, and a solvent ultrapure water. After testing, the antibacterial gel added with the natural sulforaphane extracted by the present invention exhibits excellent antibacterial performance and pH responsiveness.

Described herein are methods of treating a human child including administering to the human child a compound selected from epigallocatechin gallate (EGCG), or a derivative thereof selected from epicatechin (EC), epigallocatechin (RGC) and epicatechin gallate (ECG), sulforaphane, olive oil, phylloquinone, quercetin, safranal, hydroxtyrosol, menaquinone-4, menaquinone-7, oleocanthal, peptide Ins-1 B9-23 19CAM22R-E, peptide Ins-1 B12-23-19CAM, peptide Ins-1 B12-23 19CAM22R-E, or a combination of any of the foregoing, wherein administration of the treatment is started between birth and six months of age. Also described are methods of treating a pregnant human or a nursing human mother who has recently given birth to an infant. Further described are insulin B chain mimotope peptides.

The invention discloses a papaya-containing composition capable of improving the bioavailability of glucoraphanin and a preparation method of the papaya-containing composition. The composition comprises a broccoli seed aqueous extract, a papaya extract and a vitamin C solution, and the broccoli seed aqueous extract, the papaya extract and the vitamin C solution are mixed according to the mass ratio of (10-30): (1-5): 1; according to the composition, the utilization rate of glucoraphanin is increased, and glucoraphanin can be stably and efficiently converted into sulforaphane; the conversion rate of glucoraphanin can be up to 45%, and the preparation process, method and the like are safe and stable and are convenient to operate and produce.

The present invention relates to a nutritional targeting composition which comprises a component 1 providing glucoraphanin, a component 2 providing myrosinase and magnesium stearate and which efficiently releases sulforaphane to activate NRF2 targets. The present invention also relates to the use of the composition in the preparation of products for preventing or treating diseases or conditions which can be prevented or treated by using sulforaphane.

The invention belongs to the field of animal nutrition and feed, and particularly relates to a sulforaphane-rich aquatic animal feed and a preparation method thereof, high-purity sulforaphane powder is used as a core additive component, and is matched with various raw materials such as fish meal, soybean meal, corn protein powder, vitamins and minerals to prepare pellet feed through the steps of tempering, puffing, drying and the like. The feed prepared by the method disclosed by the invention can directly release and absorb sulforaphane in intestinal tracts of animals, has anti-oxidation and immunoregulation effects, and effectively improves the anti-stress ability, feed efficiency and growth performance of fish and shrimp aquatic animals. The feed is stable in preparation process, can be industrially popularized and has a good application prospect.

The application discloses application of sulforaphane in preparation of an antienterovirus drug and belongs to the technical field of medicines, wherein the sulforaphane can inhibit the expression of viral proteins, inhibit viral genome replication and inhibit the production of virus particles to achieve the purpose of resisting enteroviruses; specifically, the sulforaphane can inhibit the replication of enteroviruses by up-regulating the content of p53 proteins. In addition, the sulforaphane can also be used for reducing the cell morphological damage caused by enteroviruses.

The invention discloses a preparation method of SFN-loaded ZIF-8 composite hydrogel. The preparation method specifically comprises the following steps: step 1, preparing ZIF-8 nanoparticles; step 2, preparing ZIF-8 nano particles loaded with the SFN; and step 3, preparing the ZIF-8 composite hydrogel loaded with the SFN. SFN is adopted as a medicine, ZIF-8 nano-particles with osteogenesis promoting and antibacterial effects are adopted as a carrier, SFN-loaded ZIF-8 nano-drug-loaded particles are synthesized, the SFN-loaded ZIF-8 nano-particles are combined with the hydrogel, the local concentration of SFN at the periodontitis diseased region can be effectively improved, the bioavailability of SFN can be improved, the hydrogel has injectability, and the hydrogel can be used for preparing the medicine for treating periodontitis. The drug-loaded nanoparticles are sent to the deep part of a periodontal pocket, and only a minimally invasive wound is generated. The invention also discloses the SFN-loaded ZIF-8 composite hydrogel and an application of the SFN-loaded ZIF-8 composite hydrogel in preparation of a medicine for treating periodontitis.