24 results about "Sex specificity" patented technology

Provided are methods of training a model to predict a disease onset risk. Aspects of the methods include obtaining a plurality of electronic health records, dividing the subjects into a first set for machine learning model training and a second set for machine learning evaluation, training a machine learning model using the electronic health records of the first set of subjects, evaluating the machine learning model using the electronic health records of the second set of subjects and identifying from the trained and evaluated machine learning model one or more features of the subjects that predict the disease onset risk. In some embodiments, the one or more features are one or more sex-specific features. Also provided are methods of predicting a disease onset risk in a subject, methods of clinically evaluating the presence of a disease in a subject and methods of treating a subject predicted to be at risk of developing a disease. Embodiments of the invention are computer-implemented methods. Systems and non-transitory computer-readable mediums for performing the subject methods are also provided.

The invention relates to the technical field of molecular biology and aquatic product breeding, in particular to a sex specific molecular marking method of largemouth bass and application thereof, and the sex specific molecular marking method comprises the following steps: step 1, extracting genome DNA in fin ray tissues of the largemouth bass; 2, carrying out PCR (Polymerase Chain Reaction) or qPCR (Quantitative Polymerase Chain Reaction) amplification by using a sex specific primer pair; step 3, judging the gender according to an amplification result: if a specific band of 350bp-380bp is amplified in common PCR (Polymerase Chain Reaction), or a CT (Computed Tomography) value in qPCR is less than or equal to 27, judging that the sex is male; if there is no amplified band or the CT value is greater than 27, determining that the fish is female; the primer and the probe designed based on the SVER-Y region are high in specificity and low in misjudgment rate; the application range is wide, and the method is suitable for micropterus salmoides at different growth stages; qPCR can be completed within 2 hours, and the method is suitable for large-scale sample screening.

Provided herein are methods of sex sorting a plurality of insects based on sex-specific gene expression, the methods comprising (a) generating an exogenous nucleic acid molecule; (b) delivering the exogenous nucleic acid molecule into an insect body of the plurality of insects, wherein the exogenous nucleic acid molecule comprises a promoter region, a gender-specific splicing module, a reporter gene, and a transcription terminator; (c) detecting gender specific gene expression of the reporter gene; and (d) sorting the insects of the plurality of insects based on the detection of the gender-specific gene expression in step (c), thereby sex-sorting the insects based on the gender-specific gene expression.

The invention relates to the technical field of medical prognosis evaluation, in particular to a sex-specific advanced young non-small cell lung cancer prognosis model construction method, which collects clinical data of young metastatic non-small cell lung cancer patients, including sex, age, body mass index, D-2 polymer level, gene mutation state, metastasis site, tumor marker and hematology index. Data are divided into male and female queues, male specific prognostic factors (body mass index, gene mutation state and liver metastasis state) and female specific prognostic factors (D-2 polymer level and gene mutation state) are identified through Cox proportional risk regression analysis, and male and female column map prognostic models are respectively constructed based on the factors. All prognosis factors are converted into risk scores, the corresponding relation between the total score and the survival probability is established, finally, the model can output individualized survival probability prediction results of patients of different genders, and important reference is provided for clinical treatment and prognosis evaluation.

The invention belongs to the technical field of molecular marker-assisted breeding, and particularly relates to a gender-specific SNP molecular marker and a genetic gender identification method for florida softshell turtles, the SNP molecular marker is located at the 128 basic group of a nucleotide sequence as shown in SEQ ID NO.3 and / or 4, when the 128 basic group is an AA genotype, a to-be-detected florida softshell turtle individual is male, and when the 128 basic group is an AG genotype, the to-be-detected florida softshell turtle individual is female. The SNP molecular marker provided by the invention provides an important molecular tool for sex identification and sex determination mechanism research, can convert the judgment of the individual sex character of the apalone ferox into the judgment of the genotype of the molecular marker, and is beneficial to sex identification in the early growth stage of the apalone ferox; the method has the advantages of rapidness, high efficiency, short sex detection period, large detection flux, small interference of environmental factors, high accuracy of detection results, good reliability and the like, and has wide application prospects and social significance in the field of sex determination of apalone ferox.

Provided herein are methods of generating a sex-distinguishable insect population that include (a) delivering a cisgenic nucleic acid molecule into an insect genome, wherein the nucleic acid molecule comprises a sex-specific splicing module, and wherein the nucleic acid molecule is introduced into an endogenous phenotypic gene of the insect; (b) generating the sex-distinguishable insect population, wherein an insect of the sex-distinguishable insect population comprises the cisgenic nucleic acid molecule; and (c) sorting the insect from the sex-distinguishable insect population based on the differential sex-specific expression of the endogenous phenotypic gene, wherein the endogenous phenotypic gene is functionally expressed in only one sex.

The invention discloses a loach sex specific molecular marker, a primer pair, a kit and application, and belongs to the technical field of aquatic animal genetic breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. The molecular marker stably exists in a male loach genome and is deleted from female loaches. Based on this, the invention designs a primer pair for detecting the molecular marker and a kit comprising the primer pair, and provides a specific method for identifying the gender of the loach, and the method judges the gender by detecting whether a PCR amplification product exists or not: the loach is male when a specific band appears, and the loach is female when no band exists. The specific molecular marker for identifying the sex of the loach solves the problem that early-stage and non-invasive sex identification cannot be carried out in the prior art, has the advantages of being accurate, rapid and easy and convenient to operate, and has great significance in conducting sex screening and regulation on breeding populations of the loach, shortening the breeding period and improving the breeding efficiency.

This invention discloses a method for the isolation, culture, and identification of chicken sexual gonadal cells, belonging to the field of biotechnology. The invention selects chicken embryos hatched to 18.5 days old, isolates the gonads after PCR sex identification, and uses 0.25% trypsin-EDTA digestion followed by filtration through a 70μm filter to obtain a single-cell suspension with a survival rate >90% and free from PGC contamination. A sex-specific culture system is established, allowing cells to be stably passaged and maintain a sex-specific phenotype. Through morphological, qRT-PCR, immunofluorescence, and Western blot multidimensional identification, accurate identification of male testicular Sertoli cells and female ovarian granulosa cells is achieved. This method is simple to operate, has good reproducibility, and produces high cell viability and purity. It can provide a stable gonadal microenvironment for the in vitro directed differentiation of chicken primordial germ cells, significantly improving gamete induction efficiency. It is suitable for research on avian reproductive and developmental mechanisms, transgenic breeding, and germplasm resource preservation.

The present invention provides a primer, a molecular marker, an identification method and an application for identifying the sex of Sichuan-Shaanxi Taimen, the nucleotide sequence of the molecular marker is shown in SEQ ID NO: 5, and the sex marker-specific primer includes an upstream primer sequence 5′-GAACTCCCCTCCAGTTGCTA-3′; a downstream primer sequence 5′-TTGGAGCG GCAATGCAGTAT-3′. The identification method of the present invention only requires the extraction of DNA samples from fin rays, and the determination of female and male fish by the agarose gel electrophoresis bands of PCR amplification products, which causes little damage to the fish body and can be used for early sex identification of Sichuan-Shaanxi Taimen, while protecting the Sichuan-Shaanxi Taimen, while clearly understanding the sex ratio. The present invention uses the 18s gene as a reference, and can accurately determine whether the absence of the band is caused by genomic differences, avoiding misjudgment caused by the absence of the sex-specific primer band of male fish due to reasons such as sample degradation, human error, and PCR amplification failure. The method is simple, easy to implement, and low in cost, does not require tedious processes such as sequencing, and has strong operability and practicality.

The present invention provides methods of sex-sorting a plurality of insects based on sex-specific gene expression.

The invention relates to the technical field of biology, in particular to a construction method of transgenic vertebrates with sex specific sterility and fertility simultaneously and application of the transgenic vertebrates in persistent population control. According to the construction method, a sex-specific sterile gene is taken as a target gene, and a transgenic technology is utilized to enable a targeted mutagenesis system to be continuously expressed and stably inherited in vertebrates, so that the sex-specific sterile gene function is inactivated, a sex-specific sterile phenotype and a fertile phenotype are generated at the same time, and then the sex-specific sterile phenotype and the fertile phenotype are applied to persistent population control. The invention creates a population inhibition method with stable inheritance (sustainable effect) and strong universality (especially applicable to vertebrates such as fish), so that the vertebrates can induce inheritable sex-specific sterility in a target population, thereby realizing continuous and specific population inhibition.

The invention relates to the technical field of biological information processing, in particular to a sex cell automatic classification and recording method and system.The method comprises the following steps that the sex cell DNA synthesis rate is obtained through a pulse labeling method, the S period starting time and the G2 / M transition duration are extracted, the cell nucleus area and chromatin distribution are combined, XIST and SRY genes are screened, sex specific changes are analyzed, and the sex cell DNA synthesis rate is obtained. And matching a gender track, calculating an S period proportion and a G2 / M duration, outputting a gender classification label, and integrating an appearance modification data update record. According to the method, nucleotide and fluorescence microscopic imaging are introduced, the DNA synthesis rate is quantified, a characteristic trajectory is established in combination with cell cycle key nodes and nuclear morphology, gender specificity is analyzed in combination with XIST and SRY gene expression and chromosome signals, classification basis comprehensiveness is improved, a screening threshold value is set to be matched with the gender trajectory, and the accuracy of gender detection is improved. The cycle ratio is calculated, the classification accuracy is improved in combination with the confidence coefficient, genetic and epigenetic features are integrated during data storage, and subsequent analysis is supported.

The present disclosure provides insecticides that can specifically target mosquitoes based on their sex. These sex-specific insecticides prevent maturation or development of larvae into adult insects using interfering RNA (iRNA). The present disclosure further provides compositions comprising sex-linked iRNA and methods of controlling, reducing, or treating an insect infestation with the iRNA or compositions described herein. The compositions and methods described herein can be used to sort mosquitoes based on sex.

The invention belongs to the technical field of medicines, and discloses application of day lily in preparation of a product for delaying aging of a female organism. The day lily shows a remarkable anti-aging effect with sex specificity. The day lily shows a remarkable anti-aging effect with sex specificity. The day lily has a remarkable anti-aging effect on female drosophila melanogaster and has dose dependence. However, day lily has no obvious influence on the survival life of male drosophila melanogaster, and shows high female targeting. The anti-aging activity is mainly embodied in improving the survival rate in the middle stage (prolonging the healthy life) instead of simply prolonging the absolute lifetime. In addition, the day lily does not significantly improve key antioxidant indexes in the body, and it is proved that the anti-aging effect of the day lily is achieved through a mechanism independent of a traditional antioxidant pathway.

The invention provides a kelp sex specific molecular marker and application thereof, wherein the nucleotide sequence of a male marker fragment is SEQ ID NO: 1, and the nucleotide sequence of a female marker fragment is SEQ ID NO: 2. A gene sequence fragment obtained by screening is from a conserved homologous gene in a sex determining region of brown algae. The screened sex identification marker primer sequence is from a region with the highest similarity in a homologous gene coding sequence, the sex of the kelp gametophyte can be rapidly identified only through a pair of degenerate primers, the efficiency is high, the accuracy is high, and the interspecific universality is high. The method is suitable for identification of male and female mixed samples, distinguishing of parthenogenesis source and sexual reproduction source kelp sporophytes and sex identification of gametophytes of other species of kelp, and important support is provided for efficient management and research and utilization of a kelp gametophyte germplasm bank.

The present application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a SNP marker for identifying the genetic sex of Chinese soft-shelled turtles, a primer pair, a kit and application, wherein the SNP marker is located at the 106th and / or 109th base of the nucleotide sequence shown in SEQ ID NO. 3, when the 106th base is TT genotype, the to-be-tested Chinese soft-shelled turtle individual is male, and when the 106th base is TG genotype, the to-be-tested Chinese soft-shelled turtle individual is female; when the 109th base is GG genotype, the to-be-tested Chinese soft-shelled turtle individual is male, and when the 109th base is GA genotype, the to-be-tested Chinese soft-shelled turtle individual is female. The sex-specific SNP marker provided by the present application can realize the conversion of the judgment of the sex trait of the Chinese soft-shelled turtle individual into the judgment of the genotype of the molecular marker, and through the detection of the genotype of the molecular marker, the sex identification can be carried out quickly, efficiently, accurately and with high throughput at the genetic level.

The present invention provides methods for to sex-specific treatment and dose titration of diseases associated with an aberrant functionality of activity-dependent neuroprotective protein (ADNP) and / or cytoskeleton such as progressive supranuclear palsy (PSP), schizophrenia, amnestic mild cognitive impairment (aMCI), Alzheimer's disease, and autism. The treatment in these diseases, e.g., the dose and / or the regimen, differs between sexes and has to be adapted to obtain the desired effect. Specifically, use of davunetide in treatment of women suffering from PSP or of men suffering from schizophrenia or aMCI are provided.

Disclosed is a primer pair comprising nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2, where the primer pair is a PCR-based molecular marker, and where the marker is a sex-specific marker of prawns. Use of the PCR-based molecular marker of the present invention for determining the sex of prawns is disclosed. Disclosed is a method of establishing a male parsexual culture of prawns, the method comprising a PCR-based sex determination wherein the PCR-based sex determination comprises the use of a PCR-based molecular marker of the invention.

This invention provides a sex-specific molecular marker for kelp and its application, wherein the nucleotide sequence of the male marker fragment is SEQ ID NO:1, and the nucleotide sequence of the female marker fragment is SEQ ID NO:2. The gene sequence fragments obtained by this invention are derived from conserved homologous genes in the sex-determining regions of brown algae. The primer sequences for the selected sex identification markers are derived from the regions with the highest similarity in the coding sequences of the homologous genes. This method enables rapid identification of the sex of kelp gametophytes using only a pair of degenerate primers, offering high efficiency, high accuracy, and high interspecies compatibility. This method is applicable to the identification of mixed male and female samples, the differentiation of kelp sporophytes from parthenogenesis and sexual reproduction, and the sex identification of gametophytes in other species of the Laminariales order, providing important support for the efficient management and research utilization of kelp gametophyte germplasm banks.

This invention belongs to the field of marker-assisted breeding technology, and particularly relates to sex-specific SNP molecular markers and genetic sex identification methods for pearl softshell turtles. The SNP molecular marker is located at the 128th base of the nucleotide sequence shown in SEQ ID NO. 3 and / or 4. When the 128th base is the AA genotype, the tested pearl softshell turtle is male; when it is the AG genotype, the tested pearl softshell turtle is female. The SNP molecular markers provided by this invention offer an important molecular tool for sex identification and sex determination mechanism research. They can transform the determination of the sex trait of a pearl softshell turtle into the determination of the molecular marker genotype, which is beneficial for sex identification in the early stages of pearl softshell turtle growth. This invention has advantages such as speed and efficiency, short sex detection cycle, high throughput, minimal interference from environmental factors, high accuracy and reliability of detection results, and has broad application prospects and social significance in the field of pearl softshell turtle sex identification.

This invention discloses primers, kits, and methods for rapid sex identification of the peach fruit borer based on specific molecular markers. The primer pairs are based on male-specific genes of the peach fruit borer. CpunM The design incorporates the following primer sequences: 5'-AGGCTTTCGACAAGCTTCTCTA-3' and 5'-CCTGATTTGCGTGATAAGTGGT-3'. Specific PCR detection enables reliable sex identification of peach fruit borer larvae, pupae, and adults. This invention provides efficient and reliable technical support for implementing sex-specific control strategies for peach fruit borer, including sex ratio monitoring, mating behavior research, and sterile insect technology.

Provided are methods of training a model to predict a disease onset risk. Aspects of the methods include obtaining a plurality of electronic health records, dividing the subjects into a first set for machine learning model training and a second set for machine learning evaluation, training a machine learning model using the electronic health records of the first set of subjects, evaluating the machine learning model using the electronic health records of the second set of subjects and identifying from the trained and evaluated machine learning model one or more features of the subjects that predict the disease onset risk. In some embodiments, the one or more features are one or more sex-specific features. Also provided are methods of predicting a disease onset risk in a subject, methods of clinically evaluating the presence of a disease in a subject and methods of treating a subject predicted to be at risk of developing a disease. Embodiments of the invention are computer-implemented methods. Systems and non-transitory computer-readable mediums for performing the subject methods are also provided.

The invention discloses a multifunctional gender specificity physiological signal monitoring system which comprises a sensing module based on a gender specificity structure, a control module, a battery module and a display module. One side of the sensing module based on the gender specific structure is adhered to the skin surface in different scenes in different composite structure forms, and the other side of the sensing module is connected with the control module; the control module is fixed on the clothes in the form of a flexible circuit board; the battery module is directly connected to the control module to supply power to the control module; the display module is connected with the mobile phone end and the control module by turning on built-in Bluetooth of the mobile phone and then turning on a power switch of the control module, and real-time changes of specific signals monitored by the sensing module are displayed at the mobile phone end. According to the invention, abdominal uterine contraction strain and local temperature change of the pregnant woman are synchronously captured, and dual-mode dynamic evaluation of abnormal uterine activity in the gestation period is realized; meanwhile, the device can also serve as a male penis erection function monitoring ring, and psychological and organic ED are distinguished through radial strain-temperature collaborative analysis.