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72 results about "Sex specificity" patented technology

Sex-specific molecular markers and their genetic sex identification method

ActiveCN102286479AGenetic sex determination methods are accurateSensitive method for genetic sex determinationMicrobiological testing/measurementDNA/RNA fragmentationSequence analysisMultiplex polymerase chain reaction
The invention provides a striped beakfish sex specific molecular marker and a genetic sex identification method thereof. The method comprises: screening and cloning of a male specific amplified fragment length polymorphism (AFLP) marker; performing DNA sequence analysis on sex specificity; and constructing a genetic sex polymerase chain reaction (PCR) identification method. In the invention, 4 primer combinations selected from 144 pairs of primer combinations can produce 4 male specific AFLP markers; the AFLP markers are cloned and sequenced to screen out a sequence characterized amplified regions (SCAR) marker molecular marker; and thus, the genetic sex identification method for striped beakfish is established. In the invention, the AFLP method is used for the first time to screen out the sex specific molecular marker for the striped beakfish and convert the sex specific molecular marker for the striped beakfish into the SCAR marker so as to establish the genetic sex identification method for the striped beakfish. The striped beakfish genetic sex identification method has the advantages of accuracy, sensitivity, reliability and the like, has high scientific value for the study onfish sex and has a great significance and high application value for the sex control and selective breeding of fish.
Owner:MARINE FISHERIES RES INST OF ZHEJIANG

A Microsatellite Marker and Specific Primer for Identifying Female and Male Individuals of Ussuri Pseudomonas and Its Application

The invention belongs to the technical field of colony identification in aquaculture, and discloses a micro-satellite marker and specific primer for identifying female and male individuals of pseudobagrus ussuriensis, and application. One sex-specific micro-satellite site of the pseudobagrus ussuriensis is provided, and has a nucleotide sequence shown by any one of SEQ ID NO:1-2. The invention also provides a primer designed from the micro-satellite site, and the primer has a nucleotide sequence shown by SEQ ID NO:3-4. One micro-satellite site is screened out of a micro-satellite enriched library of the pseudobagrus ussuriensis, and a specific primer is designed according to flanking sequences at two ends of a micro-satellite, so that an amplification result has sex specificity, the female and male individuals of the pseudobagrus ussuriensis can be effectively identified, and the specific primer can also be used for performing real-time monitoring on sex structures of colonies in natural water areas and breeding places. The invention also discloses a kit for identifying the female and male individuals of the pseudobagrus ussuriensis. The identification method provided by the invention has the advantages of high accuracy, high resolution ratio and high speed.
Owner:HUAIYIN TEACHERS COLLEGE

Method for rapidly identifying charybdis feriata sex specific molecular marker and genetic sex

ActiveCN108588238AAccurate and efficient identification methodHigh identification accuracyMicrobiological testing/measurementDNA/RNA fragmentationSequence analysisCharybdis feriata
The invention relates to a method for rapidly identifying a charybdis feriata sex specific molecular marker and genetic sex. For SNP sites used for charybdis feriata sex identification, the base sequence is SEQ ID NO:1 or SEQ ID NO:2, and the length is 368bp; the SNP sites are the 35 site, the 89 site, the 120 site, the 218 site and the 240 site of SEQ ID NO:1 or SEQ ID NO:2. The identification method comprises the steps that charybdis feriata male and female individuals with known sex is subjected to DNA extraction; genomic library building and high throughput sequencing are simplified; sequence analysis and sex specific SNP site screening are conducted; candidate SNP site enlarged sample validation is conducted; sex specific primers based on the sex specific SNP sitesare designed; by means of the male specific primers, under specific annealing temperature, the genome DNA of charybdis feriata is subjected to PCR amplification, and specific sex determination is conducted according to the agarose gel electrophoresis detection result. A reliable method can be provided for charybdis feriata genetic sex and especially for the charybdis feriata in early developmentstage, and the method has the advantages of being easy to operate, short in consumed time, low in cost, good in repetition, high in accuracy rate and the like.
Owner:SHANTOU UNIV

Micro-satellite marker and specific primer for identifying female and male individuals of pseudobagrus ussuriensis and application

The invention belongs to the technical field of colony identification in aquaculture, and discloses a micro-satellite marker and specific primer for identifying female and male individuals of pseudobagrus ussuriensis, and application. One sex-specific micro-satellite site of the pseudobagrus ussuriensis is provided, and has a nucleotide sequence shown by any one of SEQ ID NO:1-2. The invention also provides a primer designed from the micro-satellite site, and the primer has a nucleotide sequence shown by SEQ ID NO:3-4. One micro-satellite site is screened out of a micro-satellite enriched library of the pseudobagrus ussuriensis, and a specific primer is designed according to flanking sequences at two ends of a micro-satellite, so that an amplification result has sex specificity, the female and male individuals of the pseudobagrus ussuriensis can be effectively identified, and the specific primer can also be used for performing real-time monitoring on sex structures of colonies in natural water areas and breeding places. The invention also discloses a kit for identifying the female and male individuals of the pseudobagrus ussuriensis. The identification method provided by the invention has the advantages of high accuracy, high resolution ratio and high speed.
Owner:HUAIYIN TEACHERS COLLEGE
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