14 results about "Sex specificity" patented technology

The invention belongs to the technical field of molecular marker-assisted breeding, and particularly relates to a gender-specific SNP molecular marker and a genetic gender identification method for florida softshell turtles, the SNP molecular marker is located at the 128 basic group of a nucleotide sequence as shown in SEQ ID NO.3 and / or 4, when the 128 basic group is an AA genotype, a to-be-detected florida softshell turtle individual is male, and when the 128 basic group is an AG genotype, the to-be-detected florida softshell turtle individual is female. The SNP molecular marker provided by the invention provides an important molecular tool for sex identification and sex determination mechanism research, can convert the judgment of the individual sex character of the apalone ferox into the judgment of the genotype of the molecular marker, and is beneficial to sex identification in the early growth stage of the apalone ferox; the method has the advantages of rapidness, high efficiency, short sex detection period, large detection flux, small interference of environmental factors, high accuracy of detection results, good reliability and the like, and has wide application prospects and social significance in the field of sex determination of apalone ferox.

Provided herein are methods of generating a sex-distinguishable insect population that include (a) delivering a cisgenic nucleic acid molecule into an insect genome, wherein the nucleic acid molecule comprises a sex-specific splicing module, and wherein the nucleic acid molecule is introduced into an endogenous phenotypic gene of the insect; (b) generating the sex-distinguishable insect population, wherein an insect of the sex-distinguishable insect population comprises the cisgenic nucleic acid molecule; and (c) sorting the insect from the sex-distinguishable insect population based on the differential sex-specific expression of the endogenous phenotypic gene, wherein the endogenous phenotypic gene is functionally expressed in only one sex.

The invention discloses a loach sex specific molecular marker, a primer pair, a kit and application, and belongs to the technical field of aquatic animal genetic breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. The molecular marker stably exists in a male loach genome and is deleted from female loaches. Based on this, the invention designs a primer pair for detecting the molecular marker and a kit comprising the primer pair, and provides a specific method for identifying the gender of the loach, and the method judges the gender by detecting whether a PCR amplification product exists or not: the loach is male when a specific band appears, and the loach is female when no band exists. The specific molecular marker for identifying the sex of the loach solves the problem that early-stage and non-invasive sex identification cannot be carried out in the prior art, has the advantages of being accurate, rapid and easy and convenient to operate, and has great significance in conducting sex screening and regulation on breeding populations of the loach, shortening the breeding period and improving the breeding efficiency.

This invention discloses a method for the isolation, culture, and identification of chicken sexual gonadal cells, belonging to the field of biotechnology. The invention selects chicken embryos hatched to 18.5 days old, isolates the gonads after PCR sex identification, and uses 0.25% trypsin-EDTA digestion followed by filtration through a 70μm filter to obtain a single-cell suspension with a survival rate >90% and free from PGC contamination. A sex-specific culture system is established, allowing cells to be stably passaged and maintain a sex-specific phenotype. Through morphological, qRT-PCR, immunofluorescence, and Western blot multidimensional identification, accurate identification of male testicular Sertoli cells and female ovarian granulosa cells is achieved. This method is simple to operate, has good reproducibility, and produces high cell viability and purity. It can provide a stable gonadal microenvironment for the in vitro directed differentiation of chicken primordial germ cells, significantly improving gamete induction efficiency. It is suitable for research on avian reproductive and developmental mechanisms, transgenic breeding, and germplasm resource preservation.

The present invention provides methods of sex-sorting a plurality of insects based on sex-specific gene expression.

The present disclosure provides insecticides that can specifically target mosquitoes based on their sex. These sex-specific insecticides prevent maturation or development of larvae into adult insects using interfering RNA (iRNA). The present disclosure further provides compositions comprising sex-linked iRNA and methods of controlling, reducing, or treating an insect infestation with the iRNA or compositions described herein. The compositions and methods described herein can be used to sort mosquitoes based on sex.

The invention belongs to the technical field of medicines, and discloses application of day lily in preparation of a product for delaying aging of a female organism. The day lily shows a remarkable anti-aging effect with sex specificity. The day lily shows a remarkable anti-aging effect with sex specificity. The day lily has a remarkable anti-aging effect on female drosophila melanogaster and has dose dependence. However, day lily has no obvious influence on the survival life of male drosophila melanogaster, and shows high female targeting. The anti-aging activity is mainly embodied in improving the survival rate in the middle stage (prolonging the healthy life) instead of simply prolonging the absolute lifetime. In addition, the day lily does not significantly improve key antioxidant indexes in the body, and it is proved that the anti-aging effect of the day lily is achieved through a mechanism independent of a traditional antioxidant pathway.

The present application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a SNP marker for identifying the genetic sex of Chinese soft-shelled turtles, a primer pair, a kit and application, wherein the SNP marker is located at the 106th and / or 109th base of the nucleotide sequence shown in SEQ ID NO. 3, when the 106th base is TT genotype, the to-be-tested Chinese soft-shelled turtle individual is male, and when the 106th base is TG genotype, the to-be-tested Chinese soft-shelled turtle individual is female; when the 109th base is GG genotype, the to-be-tested Chinese soft-shelled turtle individual is male, and when the 109th base is GA genotype, the to-be-tested Chinese soft-shelled turtle individual is female. The sex-specific SNP marker provided by the present application can realize the conversion of the judgment of the sex trait of the Chinese soft-shelled turtle individual into the judgment of the genotype of the molecular marker, and through the detection of the genotype of the molecular marker, the sex identification can be carried out quickly, efficiently, accurately and with high throughput at the genetic level.

This invention provides a sex-specific molecular marker for kelp and its application, wherein the nucleotide sequence of the male marker fragment is SEQ ID NO:1, and the nucleotide sequence of the female marker fragment is SEQ ID NO:2. The gene sequence fragments obtained by this invention are derived from conserved homologous genes in the sex-determining regions of brown algae. The primer sequences for the selected sex identification markers are derived from the regions with the highest similarity in the coding sequences of the homologous genes. This method enables rapid identification of the sex of kelp gametophytes using only a pair of degenerate primers, offering high efficiency, high accuracy, and high interspecies compatibility. This method is applicable to the identification of mixed male and female samples, the differentiation of kelp sporophytes from parthenogenesis and sexual reproduction, and the sex identification of gametophytes in other species of the Laminariales order, providing important support for the efficient management and research utilization of kelp gametophyte germplasm banks.

This invention belongs to the field of marker-assisted breeding technology, and particularly relates to sex-specific SNP molecular markers and genetic sex identification methods for pearl softshell turtles. The SNP molecular marker is located at the 128th base of the nucleotide sequence shown in SEQ ID NO. 3 and / or 4. When the 128th base is the AA genotype, the tested pearl softshell turtle is male; when it is the AG genotype, the tested pearl softshell turtle is female. The SNP molecular markers provided by this invention offer an important molecular tool for sex identification and sex determination mechanism research. They can transform the determination of the sex trait of a pearl softshell turtle into the determination of the molecular marker genotype, which is beneficial for sex identification in the early stages of pearl softshell turtle growth. This invention has advantages such as speed and efficiency, short sex detection cycle, high throughput, minimal interference from environmental factors, high accuracy and reliability of detection results, and has broad application prospects and social significance in the field of pearl softshell turtle sex identification.

This invention discloses primers, kits, and methods for rapid sex identification of the peach fruit borer based on specific molecular markers. The primer pairs are based on male-specific genes of the peach fruit borer. CpunM The design incorporates the following primer sequences: 5'-AGGCTTTCGACAAGCTTCTCTA-3' and 5'-CCTGATTTGCGTGATAAGTGGT-3'. Specific PCR detection enables reliable sex identification of peach fruit borer larvae, pupae, and adults. This invention provides efficient and reliable technical support for implementing sex-specific control strategies for peach fruit borer, including sex ratio monitoring, mating behavior research, and sterile insect technology.

Provided are methods of training a model to predict a disease onset risk. Aspects of the methods include obtaining a plurality of electronic health records, dividing the subjects into a first set for machine learning model training and a second set for machine learning evaluation, training a machine learning model using the electronic health records of the first set of subjects, evaluating the machine learning model using the electronic health records of the second set of subjects and identifying from the trained and evaluated machine learning model one or more features of the subjects that predict the disease onset risk. In some embodiments, the one or more features are one or more sex-specific features. Also provided are methods of predicting a disease onset risk in a subject, methods of clinically evaluating the presence of a disease in a subject and methods of treating a subject predicted to be at risk of developing a disease. Embodiments of the invention are computer-implemented methods. Systems and non-transitory computer-readable mediums for performing the subject methods are also provided.

The invention discloses a multifunctional gender specificity physiological signal monitoring system which comprises a sensing module based on a gender specificity structure, a control module, a battery module and a display module. One side of the sensing module based on the gender specific structure is adhered to the skin surface in different scenes in different composite structure forms, and the other side of the sensing module is connected with the control module; the control module is fixed on the clothes in the form of a flexible circuit board; the battery module is directly connected to the control module to supply power to the control module; the display module is connected with the mobile phone end and the control module by turning on built-in Bluetooth of the mobile phone and then turning on a power switch of the control module, and real-time changes of specific signals monitored by the sensing module are displayed at the mobile phone end. According to the invention, abdominal uterine contraction strain and local temperature change of the pregnant woman are synchronously captured, and dual-mode dynamic evaluation of abnormal uterine activity in the gestation period is realized; meanwhile, the device can also serve as a male penis erection function monitoring ring, and psychological and organic ED are distinguished through radial strain-temperature collaborative analysis.