Application of day lily in preparation of product for delaying aging of female organism

Abstract

The invention belongs to the technical field of medicines, and discloses application of day lily in preparation of a product for delaying aging of a female organism. The day lily shows a remarkable anti-aging effect with sex specificity. The day lily shows a remarkable anti-aging effect with sex specificity. The day lily has a remarkable anti-aging effect on female drosophila melanogaster and has dose dependence. However, day lily has no obvious influence on the survival life of male drosophila melanogaster, and shows high female targeting. The anti-aging activity is mainly embodied in improving the survival rate in the middle stage (prolonging the healthy life) instead of simply prolonging the absolute lifetime. In addition, the day lily does not significantly improve key antioxidant indexes in the body, and it is proved that the anti-aging effect of the day lily is achieved through a mechanism independent of a traditional antioxidant pathway.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical technology, specifically, it relates to a new use of a natural plant extract, particularly the application of daylily in the preparation of products for delaying female aging. Background Technology

[0002] Aging is a complex, multifactorial natural process characterized by the progressive decline of physiological functions and a decrease in the body's ability to adapt to environmental stresses. Developing safe and effective anti-aging intervention strategies, particularly for extending healthy lifespan, has become a key focus and hot topic in the life sciences and medical health fields.

[0003] Therefore, the development of intervention strategies to delay aging is particularly urgent and necessary. Our understanding of the molecular and genetic basis of aging has largely benefited from research on model organisms. Among them, the fruit fly, due to its short lifespan, clear genetic background, ease of manipulation, and the high degree of conservation of many core aging pathways in humans, has become a powerful tool for studying aging regulatory networks, screening potential anti-aging compounds, and assessing the effects of environmental factors (including diet).

[0004] Most existing anti-aging research and products tend to adopt a "one-size-fits-all" approach, ignoring the significant sex-specific differences in the aging process. It is well known that males and females differ fundamentally in hormone levels, metabolic patterns, and immune responses, leading to different aging trajectories and health needs. For example, the female-specific menopausal aging is closely related to the sharp decline in estrogen levels. Currently, there is a severe lack of precise intervention programs on the market that target the characteristics of female aging and work through sex-specific targets.

[0005] Early anti-aging research primarily aimed to extend lifespan. However, modern health concepts emphasize improving quality of life, namely, extending healthy lifespan and maintaining vitality and function throughout life. Therefore, interventions that improve health from middle age to old age and delay functional decline, rather than simply extending life to its final stages, have greater value and practical significance. Among various intervention pathways, nutrition and dietary patterns are considered key modifiable factors influencing the aging trajectory. Specific dietary components, through their antioxidant, anti-inflammatory, and metabolic pathway-regulating biological activities, may have a positive effect on delaying the aging process. For example, daylilies, used both as food and medicine, are rich in flavonoids, vitamins, and various trace elements; however, whether they possess anti-aging activity remains unclear. Therefore, using the model organism fruit flies to search for and screen novel active substances or compositions from natural products that can specifically improve the healthy lifespan of females through non-antioxidant pathways has significant application value and market prospects for enriching anti-aging intervention methods and meeting precise health needs. Summary of the Invention

[0006] The purpose of this invention is to provide the application of daylily in the preparation of products for delaying the aging of female organisms.

[0007] A second aspect of the present invention is to provide a food for fruit flies.

[0008] The third aspect of this invention aims to provide the application of the fruit fly food or daylily of the first aspect of this invention in the preparation of products that improve the survival rate of fruit flies.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] In a first aspect, the invention provides the use of daylily in the preparation of products for delaying aging in female organisms.

[0011] In some embodiments of the present invention, the effective dosage of daylily is 0.1% to 10%.

[0012] In some embodiments of the present invention, the effective dosage of daylily is 0.1% to 5%.

[0013] In some embodiments of the present invention, the product further includes pharmaceutically acceptable excipients.

[0014] In some embodiments of the present invention, the pharmaceutically acceptable excipients include at least one of the following: solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, flow aids, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesion agents, integrators, penetration enhancers, pH adjusters, buffers, plasticizers, surfactants, foaming agents, defoamers, thickeners, encapsulating agents, humectants, absorbents, diluents, flocculants and anti-flocculators, filter aids, release inhibitors, and carriers.

[0015] In some embodiments of the present invention, the product is at least one of a drug, food, health product, or cosmetic.

[0016] In some embodiments of the present invention, the product achieves anti-aging by improving the healthy survival process of female organisms.

[0017] In some embodiments of the present invention, the product does not achieve the purpose of anti-aging through anti-oxidation.

[0018] In some embodiments of the present invention, the organism includes fruit flies or mammals (such as humans).

[0019] In some embodiments of the present invention, the product may also include other ingredients with anti-aging activity.

[0020] A second aspect of the present invention provides a fruit fly food, comprising daylily and a fruit fly base food.

[0021] In some embodiments of the present invention, the fruit fly basic food includes corn flour, sucrose, yeast, wheat flour, agar, and propionic acid.

[0022] In some embodiments of the present invention, the amount of daylily added is 0.1% to 10% of the weight of the fruit fly basal food.

[0023] In some embodiments of the present invention, the amount of daylily added is 2% to 5% of the weight of the fruit fly basal food.

[0024] A third aspect of the present invention provides the use of fruit fly food or daylily from the second aspect of the present invention in the preparation of products that improve the survival rate of fruit flies.

[0025] In some embodiments of the present invention, the fruit fly is a female fruit fly.

[0026] In some embodiments of the present invention, the product includes pharmaceuticals, health foods, and ordinary foods.

[0027] The beneficial effects of this invention are:

[0028] This invention reveals that daylilies exhibit significant and sex-specific anti-aging effects. Daylilies show a significant anti-aging effect on female fruit flies in a dose-dependent manner. However, daylilies have no significant effect on the lifespan of male fruit flies, demonstrating high female-specific targeting. Its anti-aging activity is mainly manifested in improving mid-term survival (extending healthy lifespan), rather than simply extending absolute lifespan. Furthermore, daylilies did not significantly increase key antioxidant indicators in the body, proving that its anti-aging efficacy is achieved through a mechanism independent of traditional antioxidant pathways. Fruit flies are a recognized classic model organism in the field of aging research. Their core molecular pathways regulating lifespan and aging processes are highly conserved with those of mammals, including humans. Therefore, the effects of extending healthy lifespan and delaying aging observed in the fruit fly model in this invention provide experimental support and a reasonable expectation of success for the application of daylilies in delaying the aging of mammals (especially humans), with its efficacy mainly manifested in extending the healthy lifespan of individuals. Attached Figure Description

[0029] Figure 1 The effect of low doses of daylily (1.12%) on the lifespan of male fruit flies was investigated.

[0030] Figure 2 The effect of high doses of daylily (4.48%) on the lifespan of male fruit flies.

[0031] Figure 3The effect of low doses of daylily (1.12%) on the lifespan of female fruit flies was investigated.

[0032] Figure 4 The effect of high doses of daylily (4.48%) on the lifespan of female fruit flies.

[0033] Figure 5 The effect of daylily on the climbing ability of female fruit flies.

[0034] Figure 6 The effect of daylily on the antioxidant index of fruit flies. Detailed Implementation

[0035] The present invention will be further described in detail below through specific embodiments.

[0036] It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention.

[0037] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0038] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0039] Example 1

[0040] 1. Experimental Methods

[0041] 1.1 Experimental Subjects

[0042] Wild-type Canton S strain of Drosophila melanogaster

[0043] 1.2 Experimental Materials and Reagents

[0044] The experimental materials and reagents are shown in Table 1.

[0045] Table 1. List of materials used in the experiment

[0046]

[0047] 1.3 Experimental Instruments and Equipment

[0048] The experimental instruments and equipment are shown in Table 2.

[0049] Table 2. List of Instruments Used in the Experiment

[0050]

[0051]

[0052] 1.4 Experimental Procedure

[0053] 1.4.1 Preparation of Drosophila basal culture medium

[0054] According to the formula of Drosophila basal culture medium in Table 3, weigh out a certain amount of corn flour, sucrose, yeast, wheat flour and agar, add distilled water, heat and stir on an induction cooker, then use a pipette to add a certain amount of propionic acid to the culture medium, stir evenly and pour it into blank Drosophila tubes, and after cooling, plug the Drosophila tubes with sponge plugs to obtain the blank culture medium, which serves as the control group.

[0055] Table 3. Formulation of basal culture medium for Drosophila

[0056]

[0057] 1.4.2 Preparation of culture medium for Drosophila samples

[0058] A certain amount of dried daylily powder was added to the basal culture medium, and the daylily powder was passed through a 40-mesh sieve. Two intervention groups were prepared, one containing a low dose of daylily (1.12% of the weight of the basal culture medium) and the other containing a high dose of daylily (4.48% of the weight of the basal culture medium).

[0059] 1.4.3 Effects of daylily on the lifespan of fruit flies

[0060] To investigate the effect of daylily on the survival of male fruit flies at a specified dosage, further research was conducted on the effect of dried daylily powder on fruit fly survival. The specific experimental procedures were as follows: Male and female fruit flies that emerged within 24 hours were collected and cultured together with a standard diet for 2 days. After 2 days of co-culture, male and female fruit flies were separated, and male and female fruit flies were selected separately for the fruit fly lifespan experiment. Each tube contained 50 fruit flies of a single sex, with 6 replicates for each of the standard diet and daylily powder groups. All groups of fruit flies were placed in an artificial climate chamber (temperature 25℃, humidity 60%). The culture medium was changed every Monday, Wednesday, and Friday, and the number of dead fruit flies in each tube was recorded until all fruit flies had died.

[0061] 1.4.4 Effects of daylily on fruit fly climbing ability

[0062] This experiment employed a negative geotropism test to investigate the effect of daylily on the locomotion of fruit flies. Because fruit flies exhibit negative geotropism, they crawl in the opposite direction to gravity, exhibiting an upward climbing tendency. Flies at the bottom will slowly climb upwards. The number of fruit flies that cross a standard line was observed and recorded. The line-crossing rate was obtained by dividing the number of fruit flies that crossed the line by the total number of fruit flies. The experiment was repeated three times, with fruit fly locomotion measured on days 20 and 40 of cultivation, comparing the climbing ability of fruit flies in the control group and the daylily intervention group.

[0063] 1.4.5 Effects of daylily on the antioxidant capacity of fruit flies

[0064] The fruit flies were fed using the same method as described in 1.4.3, for 25 days. The fruit flies were then anesthetized with carbon dioxide, and groups of 15 flies were added to pre-cooled physiological saline solution and thoroughly ground. The mixture was centrifuged at 3500 rpm for 15 minutes at 4°C. The catalase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) assays were performed according to the kit instructions. Each group was repeated 4–6 times.

[0065] 1.4.6 Statistical Analysis

[0066] SPSS 20.0 was used for statistical analysis of the data. The Kolmogorov-Smirnov test was used to test the normality of the data. When the variances were homogeneous, the least significant difference (LSD) test was used to analyze differences between groups. When the variances were not homogeneous, Tamhane's T2 was used to analyze differences between groups. The Gehan-Breslow-Wilcoxon test was used to analyze the statistical significance of the data from this lifespan experiment. A p-value < 0.05 was considered statistically significant.

[0067] 2. Data Results and Analysis

[0068] 2.1 Effects of daylily on fruit fly lifespan

[0069] For male fruit flies, compared with the control group, the low-dose and high-dose daylily intervention groups had no significant effect on the lifespan of male fruit flies. Figure 1 and Figure 2 For female fruit flies, such as Figure 3 and Figure 4 As shown, daylily exhibits significant anti-aging activity against female fruit flies. Specifically, it improves mid-term survival rate (extends healthy lifespan): In the mid-term of the experiment (from day 20 to day 44), the survival rates of female fruit flies in both the low-dose and high-dose groups were significantly higher than those in the blank control group. The improvement effect in the high-dose group was particularly significant (p<0.05) and showed a clear dose-dependent effect. In the later stage of the experiment (after day 44), the survival rate curves of each experimental group tended to overlap, indicating that the extract of this invention did not significantly extend the maximum lifespan of female fruit flies.

[0070] 2.2 Effects of daylily on the climbing ability of fruit flies

[0071] Exercise capacity is an important functional indicator for measuring the body's health status and degree of aging.

[0072] This invention evaluated the effect of the extract on the locomotion ability of female fruit flies through a climbing experiment. Figure 5 As shown, climbing ability tests were conducted on female fruit flies in the high-dose group and the control group on days 20 and 40 of the experiment, respectively. The results showed no statistically significant difference in climbing index between the two groups at different time points. This result indicates that while daylily exerts its anti-aging effects, it did not cause significant changes in the locomotion ability of fruit flies. This further corroborates the uniqueness of its mechanism of action, suggesting that it may not be achieved through stimulation of the neuromuscular system.

[0073] 2.3 Effects of daylily on the antioxidant capacity of fruit flies

[0074] To investigate the mechanism of action of daylily, this invention measured key antioxidant indicators in fruit flies.

[0075] like Figure 6 As shown, in both male and female fruit flies, compared with the control group, the high-dose daylily intervention group did not show significant changes in catalase (CAT) activity, superoxide dismutase (SOD) activity, and total antioxidant capacity (T-AOC) levels. This suggests that the significant anti-aging effect of daylily on females exhibited in this invention is not achieved through traditional, universal pathways such as scavenging free radicals or enhancing the body's overall antioxidant capacity. This reveals a novel, non-antioxidant-dependent molecular mechanism of action that may be closely related to female physiological characteristics.

[0076] 3. Conclusion

[0077] This invention uses fruit flies as a model organism to study the anti-aging effects of daylilies on fruit flies through lifespan experiments, climbing experiments, and the determination of antioxidant indicators. The results show that daylilies can significantly delay the aging process in female fruit flies, but have no significant effect on males. Its effect is mainly manifested in extending "healthy lifespan" rather than "maximum lifespan," and its anti-aging effect is independent of traditional antioxidant pathways, which has not been revealed in existing technologies. These conclusions collectively provide experimental data support for the new applications of daylilies in the preparation of drugs, health foods, or functional foods for delaying female aging and improving female health.

[0078] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments, and various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof can be combined with each other unless otherwise specified.

Claims

1. Application of daylily in the preparation of products for delaying the aging of female organisms.

2. The application according to claim 1, characterized in that, The effective dosage of daylily is 0.1% to 10%.

3. The application according to claim 1, characterized in that, The product also includes pharmaceutically acceptable excipients.

4. The application according to claim 3, characterized in that, The pharmaceutically acceptable excipients include at least one of the following: solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, flow aids, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesion agents, binding agents, penetration enhancers, pH adjusters, buffers, plasticizers, surfactants, foaming agents, defoamers, thickeners, encapsulating agents, humectants, absorbents, diluents, flocculants and anti-flocculation agents, filter aids, release inhibitors, and carriers.

5. The application according to any one of claims 1 to 4, characterized in that, The product aims to delay aging by improving the healthy survival process of female organisms.

6. The application according to claim 5, characterized in that, The organisms mentioned include fruit flies and mammals.

7. A fruit fly diet consisting of daylily and fruit fly basal food.

8. The fruit fly food according to claim 7, characterized in that, The amount of daylily added is 0.1% to 10% of the weight of the fruit fly's basal food.

9. The use of the fruit fly food or daylily as described in claim 7 or 8 in the preparation of products that improve the survival rate of fruit flies.

10. The application according to claim 9, characterized in that, The fruit flies in question are female fruit flies.

Images