Primers, kits, and methods for rapid sex identification of peach fruit borer based on specific molecular markers.

By designing molecular markers and primer pairs based on male-specific expression genes of the peach fruit borer, and combining PCR amplification and electrophoresis identification methods, the problem of low efficiency in traditional morphological identification has been solved, achieving efficient and accurate sex identification, and supporting scientific research and the development of control technologies.

CN121826186BActive Publication Date: 2026-05-26PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI +1
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Patent Information

Application Number
CN202610303242.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-03-13
Publication Date
2026-05-26
Estimated Expiration
2046-03-13

AI Technical Summary

Technical Problem

Existing technologies are insufficient for accurately and quickly identifying the sex of the peach fruit borer. Traditional morphological methods are inefficient and cannot meet the needs of population monitoring and sex-specific control technologies.

Method used

Molecular markers based on male-specific expression genes of the peach fruit borer were designed, and PCR amplification was performed using specific primer pairs. Sex was then identified by agarose gel electrophoresis. Specific primer pairs and kits are provided to achieve efficient and accurate sex identification.

Benefits of technology

It enables efficient and accurate sex identification of peach fruit borer at various developmental stages, supports sex ratio monitoring and mating behavior research, and provides a basis for the implementation of sex-specific control technologies.

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Abstract

This invention discloses primers, kits, and methods for rapid sex identification of the peach fruit borer based on specific molecular markers. The primer pairs are based on male-specific genes of the peach fruit borer. CpunM The design incorporates the following primer sequences: 5'-AGGCTTTCGACAAGCTTCTCTA-3' and 5'-CCTGATTTGCGTGATAAGTGGT-3'. Specific PCR detection enables reliable sex identification of peach fruit borer larvae, pupae, and adults. This invention provides efficient and reliable technical support for implementing sex-specific control strategies for peach fruit borer, including sex ratio monitoring, mating behavior research, and sterile insect technology.
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