Specific primer for detecting bovine IGF-1 genes, fluorescent quantitative PCR detection kit of bovine GHR genes, and detection method and application of kit
A technology of IGF-1 and kit, applied in the field of genetic engineering, can solve the problems affecting the accuracy of quantification, and achieve the effects of good stability, low experimental cost and high sensitivity
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[0027] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores.
[0028] The fluorescence quantitative PCR detection kit of IGF-1 gene of the present invention is made up of following components:
[0029] 2×SYBR GREEN MIX 5mL;
[0030] Primer mixture 500μL;
[0031] Ultrapure water 5mL;
[0032] Positive control: 500 μL of standard IGF-1 gene template.
[0033] Among them, 2×SYBR GREEN MIX consists of the following components: Taq DNA polymerase 0.1 U / μL, dNTPs substrate 0.4 mmol / L, Mg 2+ 5.0 mmol / L, 100 mmol / L KCl, 20 mmol / L Tris.HCl pH8.3, 0.02% gelatin and 20 ng / mL SYBR Green dye.
[0034] Primer mixture: upstream primer 8 μmol / L, downstream primer 8 μmol / L, ...
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