A method of indentifying brucella natural infection or immunifaction for livestock
A Brucella, natural infection technology, applied in the field of proteomics, can solve the problems of human and healthy livestock threats, national and personal economic losses, inability to distinguish natural infection and immunization of livestock, etc.
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[0029]The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples are all in accordance with conventional experimental conditions, such as Sambrook et al. Molecular cloning experiment manual (Sambrook J & Russell DW, Molecular cloning: a laboratory manual, 2001), or in accordance with the conditions suggested by the manufacturer's instructions.
[0030] Example Methods for identifying Brucella naturally infected or immunized livestock
[0031] According to the current state of application of animal vaccines in my country, the main way to prevent bovine brucellosis is to inoculate sheep No. 5 Brucella vaccine (M5) and swine No. 2 Brucella vaccine (S2), while cattle infection is mainly Brucella bovis. Therefore, through the differences in the presence of Omp31 and Bp26 in different species, it is possible to distinguish Brucella bovis from non-bovine at the serolog...
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