Bovine Brucella colloidal gold antibody detection test paper strip
A technology for detection of Brucella bovis and antibodies, which is applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as non-specificity and lack of clear description of core technical characteristics, and achieves improved specificity, reduced non-specific reactions, and improved The effect of purity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0100] Example 1
[0101] ——The effect of different conditions on the size and color of colloidal gold particles
[0102] At 99mlH 2 Add 1ml of 1% chloroauric acid (HAuCl4) to O to make the final concentration of chloroauric acid 0.01%. Continue heating the chloroauric acid aqueous solution to boiling, add 1.05%, 1.2ml, 1.4ml, 1.6ml, 1.8ml, 2.0ml of 1.05% trisodium citrate aqueous solution (reducing agent) of varying volumes, continue heating for 5 minutes, use Cool in an ice water bath. The prepared colloidal gold solution should be stored at 4°C and protected from light. Take a small amount of the cooled colloidal gold solution to measure the dispersion and uniformity of the colloidal gold solution with an ultraviolet-visible spectrometer, and observe the gold particle size with a transmission electron microscope.
[0103] As a result, the amount of the original agent added is inversely proportional to the particle size of the colloidal gold, that is, the larger the amount of th...
Example Embodiment
[0106] Example 2
[0107] ——Study on the sensitivity of colloidal gold test strips
[0108] 1. Sensitivity test of the positive control serum for the positive control of Brucella bovine antibodies. After the positive control serum for the positive control of Brucella bovine antibodies (with a titer of 320 IU) is diluted by 2 times, it is tested with 3 batches of laboratory self-made colloidal gold test The paper strips were tested to determine the sensitivity of the test strips to the positive control serum diluted in gradients. The judgment time was within 4 minutes. The test results are shown in Table 2. The results show that the colloidal gold test strip of the present invention has good sensitivity and can detect the positive serum of Brucella with a titer of 2.5 IU, which is higher than the tiger red plate agglutination test and the complement fixation test.
[0109] Table 2 Sensitivity detection of positive control serum with gradient dilution
[0110]
[0111] 2. Sensitivity t...
Example Embodiment
[0115] Example 3
[0116] ——Research on the specificity of colloidal gold test strips
[0117] 1. Specific detection of Yersinia enterocolitica O9 positive serum
[0118] Three batches of Brucella bovis colloidal gold test strips prepared by our laboratory were used to detect Yersinia enterocolitica O9 positive serum. The result was that no specific band appeared at the detection line within 3 minutes. Is negative.
[0119] 2. Specific detection of known negative serum
[0120] 132 antibody-negative sera of Brucella bovis with a known background were tested with 3 batches of colloidal gold test strips. As a result, 5 of the 3 batches of kits were not detected, and the sera numbers were 12, 34, 66, 91. And 108, the specificity is 95.5%, the test results are shown in Table 4.
[0121] Table 4 The specific results of different batches of kits for negative bovine serum
[0122]
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap