Full-process biological detection device

Abstract

The invention discloses a full-process biological detection device, belonging to the technical field of biological detection. The a full-process biological detection device comprises at least one reactor, wherein the reactor comprises at least one sample adding unit, at least one controlled liquid release unit, at least one extraction unit, at least one controlled liquid switching unit, at least one liquid storage unit, at least one liquid transfer unit, at least one liquid flow control unit, at least one quantitative liquid dispersion unit and a plurality of reaction tanks, wherein all the above units communicate with each other through runners according to a preset sequence, and when the reactor rotates around a chip rotating shaft, liquid in the reactor can flow to downstream unit fromupstream unit through the runners under the driving of centrifugal force or surface tension or both the centrifugal force and the surface tension. The full-process biological detection device providedby such a scheme has the characteristics of high integration level, no need for additional reagents, simplicity in operation, stability, reliability and the like, realizes full-flow closing of a target during biological detection, and effectively avoids cross contamination between samples and equipment.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a full-process biological detection device. Background technique [0002] Microfluidic chip technology is a technology that integrates the basic operations of sample preparation, separation, reaction and detection in various biological, chemical or medical analysis processes on chips ranging from a few square centimeters to tens of square centimeters. With the characteristics of high integration, high degree of automation, and small consumption of samples and reagents, it has been widely used in many fields such as biochemical detection, nucleic acid amplification, immune analysis, cell sorting, food safety, and environmental monitoring. [0003] Patent Document 1 (CN103831140A) proposes a centrifugal-driven nucleic acid detection microfluidic chip device, which solves the problems of automatic and accurate quantitative dispersion and physical isolation of liquids, a...

AI Technical Summary

Problems solved by technology

Judging from the specific technical solution given by the patent, its practicability and feasibility are not high

First of all, the three-fork pipe connecting the nucleic acid extraction unit, the PCR amplification unit and the waste liquid unit is just a simple three-fork structure. In practice, this simple three-fork structure is likely to cause liquid to deviate from the expected direction and enter the opposite side, whether it is Whether the waste liquid enters the nucleic acid recovery unit or the nucleic acid enters the waste liquid unit will have a serious impact on the subsequent reaction and lead to the failure of the entire experimental process.

Secondly, the patent mentions that nucleic acid adsorption and elution can be achieved at a relatively low rotational speed of 1500rpm, indicating that the solid-phase adsorption matrix is ​​in a loose state, and the efficiency of nucleic acid purification and recovery is low, which can easily lead to inability to effectively recover nucleic acids and cause downstream amplification reactions failed or invalid

In addition, the chip design does not include on-chip storage of wash and eluent, which is inconvenient for practical use, and the sequential release of liquid is very unreliable

[0007] It can be seen that the current existing technical solutions have the following problems: 1) the integration degree is low, the whole process integration cannot be realized, and more manual operations are still required to complete the complete biological detection; 2) the versatility is not enough, only It can handle specific types of samples, and cannot handle biological samples with slightly complex components. The scope of application is relatively limited and cannot meet the actual inspection needs; 3) The feasibility is not high, and some designs cannot pass reasonable scrutiny or practical verification; 4) Detection ability Poor, due to design compromises or deficiencies, its actual detection performance often cannot meet the needs; 5) Many other deficiencies, such as poor sealing and practicability, have higher requirements for operators or detection equipment, different There is a high risk of cross-contamination between samples or between samples and equipment, etc.

Images