Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Brassica napus ALS (Acetolactate Synthase) gene promoter and application

A Brassica napus and promoter technology, applied in application, genetic engineering, recombinant DNA technology, etc., to achieve the effect of reducing biosafety risks

Inactive Publication Date: 2017-12-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research and application of the ALS gene promoter has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Brassica napus ALS (Acetolactate Synthase) gene promoter and application
  • Brassica napus ALS (Acetolactate Synthase) gene promoter and application
  • Brassica napus ALS (Acetolactate Synthase) gene promoter and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] 1. Promoter sequence cloning and promoter element analysis

[0029] The present invention is based on the ALS of rapeseed M342 3 Gene sequence, use NCBI website (https: / / www.ncbi.nlm.nih.gov / ) to retrieve its corresponding rapeseed genome sequence, extract ALS 3 For the sequence of about -2000 bases upstream of the 5' end of the gene coding region, use the professional website (http: / / molbiol-tools.ca / Promoters.htm) for promoter prediction analysis, and select rich promoter elements according to the analysis results Design specific amplification primers for the upstream -1000 base sequence. ALS 3 The upstream sequence of the gene is used as a template, and the downstream primer is ALS of M342 3 The 5' end non-coding region sequence of the gene is used as a template to amplify ALS 3 The DNA sequence of the upstream of the gene is about 1048bp in size.

[0030] 1.1 Amplification and cloning of the promoter sequence: Using the genomic DNA of herbicide-resistant rapese...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a brassica napus ALS (Acetolactate Synthase) gene promoter and application and belongs to the technical field of biology. The brassica napus ALS gene promoter disclosed by the invention is characterized in that a brassica napus ALS gene promoter sequence is colonized; a promoter element contained in the sequence is predicted by bioinformatics software; the activity of the gene promoter is subjected to qualitative detection and analysis by constructing a plant expression vector; and the results show that the colonized sequence can drive a GUS reporter gene to be expressed in all organs of tobacco seedlings, has high expression activity and characteristics of a constitutive promoter and can be applied to plant genetic engineering research as the strong constitutive expression promoter. The promoter can replace a cauliflower mosaic virus (CaMV) 35S promoter and an agrobaeterittm tumefaciens nopaline gene nos promoter which are normally used in the current plant genetic engineering research to construct a plant transgenic expression vector; and the plant transgenic expression vector is applied to genetic engineering of dicotyledons, so that the biological safety risk of transgenic plants is reduced.

Description

technical field [0001] The invention relates to a brassica napus ALS gene promoter and its application, and belongs to the field of biotechnology. Background technique [0002] Rapeseed is one of the most important oil crops in the world, and it is also an important oil crop in my country. my country's rapeseed planting area and total output both rank first in the world. In the 1960s and 1970s, the annual planting area of ​​rapeseed in my country was about 30 million mu, with a yield of 30-40 kg per mu; in the 1990s, it had grown to 100 million-110 million mu, with a yield of 90-100 kg per mu, and the total rapeseed output reached 1100- More than 12 million tons. Rapeseed is the fifth largest crop in my country after rice, wheat, corn, and soybean (it will surpass soybean soon, and rapeseed is the second largest crop after rice in provinces such as Hubei in the Yangtze River Basin), and it is also the only winter oil crop. my country's rapeseed area and total output accoun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC12N9/1022C12N15/8222C12Y202/01006
Inventor 陈松浦惠明彭琦张洁夫陈锋王晓东张维胡茂龙龙卫华高建芹付三雄周晓婴熊冬琴
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com