This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid
electrolysis and
highly sensitive electrochemical
nucleic acid detection of
Cryptococcus neoformans and its application. The micro-platform includes an integrated
microfluidic chip, an external power supply, a sample introduction
system, and / or an imaging
system. The integrated
microfluidic chip includes an
electrolysis region, an
electrochemical detection region, and an
electrode array region. The
electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the
electrode pairs above them. The connection point between every two electroporated channels is the
electroporation lysis point. The
electrochemical detection region consists of a
working electrode, a
reference electrode, and a counter
electrode. The
working electrode is modified with rGO / AuNPs
nanomaterials and molecular probes.