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35results about How to "Determination of precision" patented technology

Device for analyzing optical fiber path characteristics and method for analyzing same

In the present invention, with respect to an optical fiber path (100) to be tested that branches a backbone optical fiber (F0) into N systems of branched optical fibers (F1-FN) by means of an optical splitter (SP), an optical reflection filter (R1-RN) is disposed at each end of the branched optical fibers (F1-FN), a test optical pulse is generated from a first and second test optical pulse at a predetermined time difference from a first and second test light having differing wavelengths, by means of an optical circulator (21), the test optical pulse is caused to enter the backbone optical fiber (F0) of the optical fiber path (100) to be tested, and the return light exiting from the entrance end is taken out, the stimulated Brillouin backscattering light is extracted by an optical filter (22), and the scattering light is received by an optical receiver (23); and converted to an electrical signal, and the electrical signal is digitally processed while chanting the time difference of the first and second test optical pulses by means of a computational processing device (25), by which means it is determined which branched optical fibers (F1-FN) the stimulated Brillouin scattering light is from, and the characteristic distribution for each branched optical fiber is determined.
Owner:NIPPON TELEGRAPH & TELEPHONE CORP

Method for measuring content of 5-fluorouracil in plasma and colorectal cancer cells based on high performance liquid chromatography

The invention relates to a method for measuring content of 5-fluorouracil in plasma and colorectal cancer cells based on high performance liquid chromatography and belongs to the technical field of liquid chromatography medical analysis. 5-fluorouracil (5-Fu) is a miazines antineoplastic drug widely applied clinically at present; the antineoplastic chemotherapy effect of 5-Fu is related to the concentration of 5-Fu in the cancer cells; other side effects are caused when the drug concentration in the body is ultrahigh. For the purpose of enhancing the drug therapeutic effect and reducing the side effects, the trend of the concentration of 5-Fu in the cancer cells and the drug concentration in human body shall be strictly monitored. The monitoring has significant clinical significance in promotion of drug therapeutic effect, reduction of toxicity and prevention of post-operation transfer. According to the method provided by the invention, 5-Fu in plasma and colorectal cancer cells is extracted in the manner of solid phase extraction, so that the extraction efficiency is increased; the chromatographic condition is optimized; the peak pattern is improved by adding a trifluoroacetic acid and methyl alcohol system into a detected flow phase; the number of theoretical plates is increased and the chromatographic condition is mild; under a selected optimal chromatographic condition, the measurement for 5-Fu is more precise and accurate than the measurement in literature reports.
Owner:JIANGSU INST OF NUCLEAR MEDICINE

A rapid nondestructive testing method for the preservation state of water-saturated wooden cultural relics

The invention relates to the technical field of a method for determining the preservation state of water-saturated wooden cultural relics, and provides a rapid non-destructive testing method for the preservation state of water-saturated wooden cultural relics. The water-saturated wooden cultural relics are tested; the data acquisition step is to obtain the spectral data after the spectrometer test, and the spectral data is processed; the establishment of a calibration model step is used to establish a calibration model for the preservation state of the water-saturated wooden cultural relics; the preservation state step is determined, It is used to test the spectral data and clustering behavior of water-saturated wooden artifacts through the aforementioned steps, and determine their preservation status. With this technical solution, after the testing step and the data acquisition step, the steps of establishing a calibration model and determining the preservation state can be used to accurately determine the preservation state of the water-saturated wooden cultural relics, and it has the advantages of no destructive sampling damage and fast test speed. The advantages.
Owner:INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY

atp bioluminescent lgc b -lgi b Method for evaluating fungicidal effect of liquid disinfectant by standard curve method

The invention relates to a method for evaluating the fungus killing effect of a liquid disinfectant. The evaluation steps include: sample extraction and determination of the minimum effective concentration; The live bacteria content of bacterial suspension after staining C B Blood count and inoculum C B Calibration; lgC B ‑ Relative fluorescence intensity log value lgI B Standard song establishment; neutralizer identification and quantitative suspension test; recovery solution I B Determination and its live bacteria content C B and T B Calculation; Calculation of killing rate R and sterilization index A; Judgment of results; The special feature is: the use of ATP fluorescence photometer to accurately and quantitatively evaluate the fungal killing effect of disinfectants characterized by R or A. The present invention stipulates to reclaim the test sample liquid and the control sample liquid after the different time of action, measure its I B and with lgI B Characterize and calculate R or A; provide the basis for judging the results. ATP bioluminescent IgC for the evaluation of the fungal killing effect of the disinfectant developed by the present invention B ‑lgI B The standard music method can improve the relevant evaluation method system of disinfection effect.
Owner:李文杰

Needle probe for cooling operation and cooling operation system

Provided are a needle probe for cooling operation and a cooling operation system, in which a very small area can be precisely cooled. A needle probe (10) for cooling operation includes a first cylindrical body (14) and a second cylindrical body (12) made of thin metals of different kinds and extending in a state of being electrically insulated from each other. The first cylindrical body (14) and second cylindrical body (12) serve as channels for refrigerant as well as compensation conduction wires for a thermocouple. One end of each of the first cylindrical body (14) and second cylindrical body (12) is closed and bonded to form a tip end portion (18). The tip end portion (18) serves as a temperature measurement contact of a thermocouple. A very small interconnection portion (20) for interconnecting the inside and outside of the first cylindrical body (14) is formed near the tip end portion (18). Refrigerant is introduced into the first cylindrical body (14), and when the refrigerant is passed through the very small interconnection portion (20) and introduced to the second tubular body (12), the refrigerant evaporates or adiabatically expands, whereby cold heat usable for cooling the tip end portion (18) is generated. The tip end portion (18) is applied to a target area to be treated, thereby cooling the target area.
Owner:NAT UNIV CORP KYUSHU INST OF TECH (JP)

Method for evaluating fungus destroying effect of daily chemical products by ATP biofluorescence lgC-lgI standard curve method

PendingCN110272963ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementBiotechnologyStaining
The invention relates to a method for evaluating the fungus destroying effects of daily chemical products. The method comprises the detection steps of meeting requirements of sample extraction and pretreatment; performing type selection of equipment, and compounding a reagent and a culture medium; performing strain preservation, performing strain activation and preparing fungus suspension; after methylthioninium chloride staining, counting the content C of viable fungi in the fungus suspension through a hemocytometer; establishing a standard curve of ATP concentration log lgC-relative fluorescence intensity log lgI, and performing calibration on C of viable fungi in inoculated fungus liquid; performing neutralizer identification and performing fungus destroying experiment; determining I of recovered liquid, and reckoning C and T of the recovered liquid; calculating fungus destroying rate R and fungus destroying index A; and performing result evaluation. The method has the characteristic that the fungus destroying effects of the daily chemical products, which are expressed by R or A, can be accurately and quantitatively evaluated through an ATP fluorophotometer. Control samples and experiment samples after special time of fungus infection are recovered according to the specification of the invention, the I of the recovered liquid of the control samples and the I of the recovered liquid of the experiment samples are determined and expressed by lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the fungus destroying properties of the daily chemical products, researched and developed by the invention, product quality increase is supported through an advanced detection technique.
Owner:李文杰

Method for detecting anti-mildew property of antibacterial wood board by ATP bio-fluorescence lgCA-lgIA standard curve method

InactiveCN110358806ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementSporeElution
The invention relates to a method for detecting anti-mildew property of an antibacterial wood board, and the detecting steps include: sample preparation and pre-treatment; equipment selection, and reagent and medium preparation; species preservation and activation, and preparation of spore liquid; establishment of OD value-live bacteria content logarithmic value lgCB standard curve; establishmentof ATP concentration logarithmic value lgCA-relative fluorescence intensity logarithm lgIA standard curve and inoculated bacterial liquid live bacteria CA calibration; sample inoculation, culture andelution recovery; IA determination of recovered liquid and its live bacteria ATP concentration CA and TA reckoning; antibacterial rate R or antibacterial activity value A calculation; and results evaluation. The method is characterized in that an ATP fluorescence photometer is used to accurately and quantitatively test the mildew resistance of the wood board characterized by R or A. The method prescribes the elution and recovery of a control sample and an antibacterial sample after contact for 0 h and culture for 48 h, and the recovered liquid IA is determined and R or A is characterized and calculated by lgIA. The ATP bio-fluorescence lgCA-lgIA standard curve method supports the product quality improvement by a scientific and advanced detection technology.
Owner:INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Method for detecting mold resistance of antibacterial rubber by ATP biofluorescence lgC-lgI standard curve method

InactiveCN110272946ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementAntibacterial activityBiology
The invention relates to a method for detecting mold resistance of antibacterial rubber. The method comprises the detection steps of preparing and preprocessing samples; selecting equipment and preparing a reagent and a culture medium; performing strain preserving and activating and preparing bacterium suspension; establishing a standard curve of the OD value-viable bacterium content log lgC, and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of lgC-relative fluorescence intensity log lgI; performing sample inoculating, culturing, eluting and recovering; performing I determination of the recovered liquid, and calculating viable bacterium content C and T; calculating antibacterial rate R or antibacterial activity value A; and performing result evaluation. The method has the characteristics that the mold resistance, expressed by R or A, of the rubber is subjected to pinpoint quantitative test through an ATP fluorophotometer. Control samples and antibacterial samples after contact for 0h and culturing for 48h are eluted and recovered according to the specification of the invention, the I of recovered liquid is measured and expressed with lgI, and R or A is calculated. A result evaluation base is provided. The ATP biofluorescence lgC-lgI standard curve method for detecting the mold resistance of the antibacterial rubber, which is researched and developed, supports improvement of product quality by a scientific and advanced detection technique.
Owner:李文杰

Method for detecting anti-mildew performance of an antibacterial coating by ATP biological fluorescence lgC-lgI standard curve method

PendingCN110272958ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementBiotechnologySpore
The invention relates to a method for detecting the anti-mildew performance of an antibacterial coating. The method comprises the following detection steps: sample extraction, preparation and pretreatment; equipment selection, and reagent and culture medium preparation; strain preservation and activation, and spore suspension preparation; establishment of a standard curve of OD value-viable content log value lgC; establishment of a standard curve of ATP concentration log value lgC-relative fluorescence intensity log value lgI, and calibration of living bacteria C of an inoculation bacterial liquid; sample inoculating and culturing, and eluting recovery; determination of recovery liquid I, and calculation of living bacteria ATP concentration C and T; calculation of antibacterial rate R or the antibacterial activity value A; and result evaluation. The method is characterized in that: an ATP fluorophotometer is used for accurately and quantitatively testing the anti-mildew performance of a coating characterized by R or A. The method comprises the following steps: eluting and recovering a control sample and an antibacterial sample after contacting for 0h and culturing for 48h, determining recovery liquid I, and characterizing and calculating R or A by the lgI. The ATP biological fluorescence lgC-lgI standard curve method for detecting the anti-mildew performance of an antibacterial coating developed by the invention can support the product quality improvement by using an advanced detection technology.
Owner:INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Method for evaluating fungi destroying effects of disposable sanitary accessories by ATP fluorescence lgC-lgI standard curve method

The invention relates to a method for evaluating the fungus destroying effects of disposable sanitary accessories. The method comprises the detection steps of determining requirements of sample extraction and the like; performing type selection of equipment, and compounding a reagent and culture mediums; performing strain preservation, performing strain activation and preparing bacterium suspension; counting the viable bacteria content C of bacterium suspension after methylene blue dyeing with a hemocytometer, and performing calibration on C of inoculation bacterial liquid; establishing a standard curve of the lgC-relative fluorescence intensity logarithm value lgI ; performing neutralizer identification and performing bacterium destroying experiment; determining I of recovery liquid, and reckoning viable bacteria content C and viable bacteria content T; calculating bacterium destroying rate R and bacterium killing index A; and performing result evaluation. The method has the characteristic that the fungus destroying effects which are represented by R or A, of the sanitary accessories can be accurately and quantitatively evaluated through an ATP fluorophotometer. Control samples and experiment samples after fungus infection for different time are eluted and recovered according to the specification of the invention, the I of the recovery liquid is determined and represented by lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the fungus destroying properties of the disposable stainless steel, researched and developed by the invention, product quality increase is supported through an advanced detection technique.
Owner:李文杰

Method for detecting mold resistance of antibacterial plastic by ATP biofluorescence lgC-lgI standard curve method

InactiveCN110272943ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementAntibacterial activityBiology
The invention relates to a detection method for mold resistance of antibacterial plastic. The detection method comprises the detection steps of preparing and preprocessing samples; performing equipment selection and preparing a reagent and a culture medium; preserving and activating strains, and preparing a bacterium suspension; establishing a standard curve of OD value-viable bacteria content log lgC , and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of lgC-relative fluorescence intensity log lgI ; performing sample inoculating, culturing, eluting and recovering; performing I determination of the recovered liquid, and reckoning the viable bacteria content C and T; calculating antibacterial rate R or antibacterial activity value A; and performing result evaluation. The method has the characteristics that the mold resistance, expressed by R or A, of the plastic is subjected to pinpoint quantitative test through an ATP fluorophotometer. Control samples and antibacterial samples after contact for 0h and culture for 48h are eluted and recovered according to the specification of the invention, the I of recovered liquid is measured and expressed with lgI, and R or A is calculated. A result assessment basis is provided. The ATP biofluorescence lgC-lgI standard curve method for detecting mold resistance of the antibacterial plastic, which is researched and developed by the invention, supports improvement of product quality by a scientific and advanced detection technique.
Owner:李文杰

Method for detecting anti-mildew property of antibacterial plastics with ATP (adenosine triphosphate) bioluminescence lgCA-lgIA standard curve method

InactiveCN110283874ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementAntibacterial activityBiological activation
The invention relates to a method for detecting anti-mildew property of antibacterial plastics. The method comprises detection steps as follows: sample preparation and pretreatment; selection of equipment type and preparation of reagents and culture media; strain preservation and activation and preparation of a spore solution; establishment of an OD value-viable content logarithm value lgCB standard curve; establishment of an ATP (adenosine triphosphate) concentration logarithm value lgCA-relative fluorescence intensity logarithm value lgIA standard curve and calibration of viable bacteria CA of an inoculant liquid; inoculation, culture and eluting recovery of samples; IA determination of a recovery liquid and calculation of viable bacteria ATP concentration CA and TA of the recovery liquid; calculation of antibacterial ratio R or antibacterial activity value A; result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurate and quantitative test of the anti-mildew property of R or A characterized antibacterial plastics. According to the method, a control sample and an antibacterial sample after 0 h contact and 24 h culture are eluted and recovered, and IA of the recovery liquid is measured and R or A is characterized by lgIA and calculated; result evaluation basis is provided. According to the developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the anti-mildew property of the plastics, product quality improvement is supported with a scientific and advanced detection technology.
Owner:李文杰

Method for detecting barium ions in oil field water

The invention discloses a method for detecting barium ions in oil field water. The method comprises the following steps: taking an oil-water sample; adding hydrochloric acid solution and methyl red indicator into the sample; adjusting the solution to yellow by ammonia solution; adding acetic acid-ammonium acetate buffer solution into the mixture; heating and boiling the mixture; dripping potassium chromate solution into the mixture; boiling the mixture once more; adding the acetic acid-ammonium acetate buffer solution into the mixture; standing the mixture to room temperature; filtering and abandoning initial filtrate; taking the filtrate; adding distilled water into the filtrate; adding potassium iodide and sulfuric acid solution into the mixture; shaking the mixture evenly, and placing the mixture in a dark position; titrating the mixture by sodium thiosulfate standard solution; adding starch indicating solution into the mixture; recording consumption V of the sodium thiosulfate solution; and calculating the concentration of the barium ions in the oil field through the formula of the consumption V0 of the recorded sodium thiosulfate solution. By adopting the method for detectingthe barium ions, the concentration of the barium ions in the oil field can be accurately and precisely detected, and the requirement of quick and accurate analysis on scaling ions Ba<2+> can be met.
Owner:YANCHANG OIL FIELD

Device and method for rapidly detecting multiple parameters of water body

The invention discloses a device and a method for rapidly detecting multiple parameters of a water body. The device comprises a micro-fluidic chip module, which comprises a dielectrophoresis bacterium sorting chip module, an impedance bacterium testing chip module, an extraction fluorescence testing chip module and an electrophoresis ion chip module; wherein the micro-fluidic chip module is provided with four inlets, the inlet I is used as a sample inlet, and the inlet II, the inlet III and the inlet IV are respectively used as a buffer solution inlet I, a buffer solution inlet II and a buffer solution inlet III. According to the device, the micro-fluidic technology is adopted, accurate flow direction control over liquid is achieved through the micro-channels and the micro-valves, a plurality of pretreatment and detection modules are highly integrated on a small plastic sheet, and the device has high integration level and efficient mass transfer and detection speed. The whole device has the advantages of high portability, high detection speed and high timeliness. In the invention, a plurality of modules are integrated on the same chip, joint detection of multiple parameters is realized, and only one sample needs to be input, so that the detection accuracy is higher.
Owner:UNIV OF SCI & TECH OF CHINA

Device and method for detecting air permeability of mildew-proof roll paper for glass stacking

The invention provides an air permeability detection device for mildew-proof roll paper for ultrathin glass stacking. The device comprises a table surface plate, wherein a plurality of stand columns are arranged above the table surface plate, a top plate is arranged at the upper ends of the stand columns in a supporting mode, at least two push cylinders are arranged on the top plate in a penetrating mode, the push rods of the push cylinders face downwards and are fixedly connected with lifting blocks, pressing wheels are arranged at the bottoms of the lifting blocks, a negative pressure coveris arranged at the bottom of the table surface plate and is connected with a negative pressure tank through a first pipeline, a first stop valve is arranged on the first pipeline, a digital display pressure gauge is arranged on the negative pressure tank, and a plurality of groups of through holes positioned in the coverage area of the negative pressure cover are formed in the table surface plate.By the adoption of the structure, rapid and accurate air permeability detection can be conducted on mildew-proof roll paper, and the situation that ultra-thin glass falls off in the stacking processdue to the fact that the air permeability does not meet the requirement is avoided.
Owner:YICHANG CSG PHOTOELECTRIC GLASS +1

Rapid nondestructive testing method for preservation state of water-saturated wooden cultural relic

The invention relates to the technical field of methods for determining the preservation state of a water-saturated wooden cultural relic, and provides a rapid nondestructive testing method for the preservation state of the water-saturated wooden cultural relic, which comprises the following steps: a cleaning step: cleaning the surface of the water-saturated wooden cultural relic; a testing step: testing the waterlogged wooden cultural relics by using a spectrograph; a data acquisition step: acquiring spectral data after the test of the spectrograph, and processing the spectral data; a correction model establishing step: establishing a correction model of the preservation state of the waterlogged wooden cultural relic; and a storage state determination step: testing spectral data and clustering behaviors of the water-saturated wooden cultural relics through the previous steps, and determining the storage state of the water-saturated wooden cultural relics. By adopting the technical scheme, after the test step and the data acquisition step, the step of establishing the correction model and the step of measuring the preservation state are adopted, so that the preservation state of the water-saturated wooden cultural relic can be accurately measured, and the method has the advantages of no destructive sampling damage and high test speed.
Owner:INST OF WOOD INDUDTRY CHINESE ACAD OF FORESTRY

A device and method for measuring droplet deposition amount based on metal ion concentration detection

The invention relates to a fogdrop deposition amount measuring device based on metal ion concentration detection. The fogdrop deposition amount measuring device comprises a collection module, an ion sensing detection module, a processing analysis system, a wireless transmission module and a power supply module. The collection module comprises a fogdrop collection box, a deionized water storage chamber, a mounting fixing box and a sampling fixing device, the fogdrop collection box is a container for receiving the fog drops sprayed by a plant protection machine and is mounted together with the mounting and fixing box, and the lower part of the mounting and fixing box is communicated with the fogdrop collecting box. The ion sensing and detecting module is mounted in the mounting and fixing box, and the processing and analyzing system, the wireless transmission module and the power supply module are mounted at the top of the mounting and fixing box. The invention provides a droplet deposition amount measuring device and method based on metal ion concentration detection, and by combining with a calibration database, the relationship between concentration and deposition amount is calculated quickly and accurately, so that the droplet deposition amount is obtained, and the data is stored and displayed in real time.
Owner:NANJING AGRI MECHANIZATION INST MIN OF AGRI

Method for detecting mold resisting properties of electrical household appliances by ATP biofluorescence lgC-lgI standard curve method

InactiveCN110272967ADetermination of precisionBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementCompound aAntibacterial activity
The invention relates to a method for detecting the mold resisting properties of electrical household appliances. The method comprises the detection steps of preparing samples and performing pretreatment: performing type selection of equipment, and compounding a reagent and a culture medium; performing strain preservation, performing strain activation and preparing bacterium suspension; establishing a standard curve of OD value-viable bacterium content log lgC, and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of lgC-relative fluorescence intensity log lgI; performing sample inoculation, performing sample culture and performing eluting and recovering; determining I of recovered liquid, and reckoning the content C and T of the viable bacteria; calculating bacterium resisting rate R or bacterium resisting activity value A; and performing result evaluation. The method has the characteristic that the mold resisting properties of the electrical household appliances, which are expressed by R or A, can be accurately and quantitatively tested through an ATP fluorophotometer. Control samples and bacterium resisting samples after contact for 0h and culturing for 48h are eluted and recovered according to the specification of the invention, the I of the recovered liquid of the control samples and the I of the recovered liquid of the bacterium resisting samples are determined and are expressed with lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the mold resisting properties of the electrical household appliances, researched and developed by the invention, product quality increase can be supported through a scientific advanced detection technique.
Owner:李文杰

Method for detecting anti-fungal performance of coated antibacterial glass by ATP bioluminescence lgC-lgI standard curve method

InactiveCN110272952ADetermination of precisionBreak through the limitation of mold qualitative analysisFungiMicrobiological testing/measurementBiotechnologyStaining
The invention relates to a method for detecting anti-fungal performance of coated antibacterial glass. The method for detecting the anti-fungal performance of the coated antibacterial glass comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation and excitation and bacterium suspension preparation are carried out; viable bacterium content C blood cell plate counting of a bacterium suspension and inoculating bacterium solution C calibration after methylene blue staining are carried out; an lgC-relative fluorescence intensity log value lgI standard curve is established; sample inoculating, cultivating, eluting and recycling are carried out; recycled solution I measurement and viable bacterium content C and T calculation of a recycled solution are carried out; the antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the anti-fungal performance of the coated antibacterial glass is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the glass anti-fungal performance represented by R or A. According to the method for detecting the anti-fungal performance of the coated antibacterial glass, it is stipulated that control samples and antibacterial samples are recovered by elution after contact for 0h and cultivating for 24h / 48h and recycled, and a recycling solution I is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting anti-fungal performance of the coated antibacterial glass, an advanced detection technology can be used for supporting product quality improvement.
Owner:李文杰

Method for evaluating fungus destroying effect of liquid disinfecting agent by ATP biofluorescence lgC-lgI standard curve method

PendingCN110272954AHuman factors have little influenceBreakthrough operationsMicrobiological testing/measurementFluorescence/phosphorescenceStandard curveTest sample
The invention relates to a method for evaluating the fungus destroying effect of a liquid disinfecting agent. The method comprises the detection steps of performing sample extraction and determining the lowest effective concentration of a disinfecting agent; performing type selection of equipment and preparing a reagent and a culture medium; performing strain preserving and activating and preparing fungus suspension; after methylthioninium chloride staining, counting the content C of viable fungi in the fungus suspension through a hemocytometer; establishing a standard curve of the ATP concentration log lgC-relative fluorescence intensity log lgI and performing calibration on C of viable fungi in inoculated fungus liquid; performing neutralizer identification and quantitative suspension test; performing I determination and C and T reckoning on recovered liquid; calculating destroying rate R and fungus destroying index A; and performing result judgment. The method has the characteristics that the fungus destroying effect of the disinfecting agent, expressed by R or A, is subjected to pinpoint quantitative evaluation through an ATP fluorophotometer. The I of test sample liquid and the I of control sample liquid after different time of action are regulated and determined and expressed with lgI, R or A is calculated, and a result judgment basis is provided. The ATP biofluorescence lgC-lgI standard curve method for evaluating the fungus destroying effect of the liquid disinfecting agent, which is researched and developed, can perfect a relevant evaluation method system.
Owner:李文杰

Method for detecting mold resistance of antibacterial paint by ATP biofluorescence lgC-lgI standard curve method

The invention relates to a detection method for mold resistance of antibacterial paint. The detection method comprises the detection steps of performing sample extraction, preparing and preprocessing; performing equipment selection and preparing a reagent and a culture medium; preserving and activating strains, and preparing a bacterium suspension; establishing a standard curve of the OD value-viable bacteria content log lgC, and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of a lgC-relative fluorescence intensity log lgI ; performing sample inoculating, culturing, eluting and recovering; performing IB determination of the recovered liquid, and reckoning the viable bacteria content C and T; calculating antibacterial rate R or antibacterial activity value A; and performing result evaluation. The method has the characteristics that the mold resistance, expressed by R or A, of the paint is subjected to pinpoint quantitative test through an ATP fluorophotometer. Control samples and antibacterial samples after contact for 0h and culture for 48h are eluted and recovered according to the specification of the invention, the I of recovered liquid is measured and expressed with lgI, and R or A is calculated. A result assessment basis is provided. The ATP biofluorescence lgC-lgI standard curve method for detecting mold resistance of the antibacterial paint, which is researched and developed by the invention, supports improvement of product quality by a scientific and advanced detection technique.
Owner:李文杰

Method for detecting mildew-proofing performance of household electric appliances by ATP bio-fluorescence lgCA-lgIA standard curve method

InactiveCN110272977AHuman factors have little influenceBreak through the limitations of qualitative analysisFungiMicrobiological testing/measurementStandard curveCulture mediums
The invention relates to a method for detecting the mildew-proofing performance of household electric appliances. The method comprises the following detection steps: preparing and pre-treating a sample; selecting equipment models and preparing a reagent and a culture medium; preserving and activating strains and preparing a spore solution; establishing an OD value-viable content logarithm value lgCB standard curve; establishing an ATP concentration logarithm value lgCA-relative fluorescence intensity logarithm value lgIA standard curve and calibrating viable bacteria CA of an inoculating bacterial solution; performing sample inoculation, cultivation and eluting reclamation; detecting IA of reclaimed solution and estimating CA and TA of ATP concentration of living bacteria of the reclaimed solution; calculating an antibacterial ratio R or antibacterial activity A; and evaluating the result. The method is characterized in that the R or A represented mildew-proofing performance of household electric appliances can be accurately and quantitatively tested by applying an ATP fluorophotometer. According to the method, a control sample and an antibacterial sample after 0 hour of elution reclamation contact and 48 hours of cultivation are specified, the IA of the reclaimed solution is detected, and R or A is represented and calculated by lgIA. According to the ATP bio-fluorescence lgCA-lgIA standard curve method for detecting the mildew-proofing performance of household electric appliances, product quality improvement is supported by scientific and advanced detection techniques.
Owner:李文杰
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