Method for detecting mold resistance of antibacterial rubber by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting mold resistance of antibacterial rubber. The method comprises the detection steps of preparing and preprocessing samples; selecting equipment and preparing a reagent and a culture medium; performing strain preserving and activating and preparing bacterium suspension; establishing a standard curve of the OD value-viable bacterium content log lgC, and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of lgC-relative fluorescence intensity log lgI; performing sample inoculating, culturing, eluting and recovering; performing I determination of the recovered liquid, and calculating viable bacterium content C and T; calculating antibacterial rate R or antibacterial activity value A; and performing result evaluation. The method has the characteristics that the mold resistance, expressed by R or A, of the rubber is subjected to pinpoint quantitative test through an ATP fluorophotometer. Control samples and antibacterial samples after contact for 0h and culturing for 48h are eluted and recovered according to the specification of the invention, the I of recovered liquid is measured and expressed with lgI, and R or A is calculated. A result evaluation base is provided. The ATP biofluorescence lgC-lgI standard curve method for detecting the mold resistance of the antibacterial rubber, which is researched and developed, supports improvement of product quality by a scientific and advanced detection technique.

Description

technical field [0001] The invention relates to a method for testing the anti-mold performance of antibacterial rubber, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the anti-mold performance of rubber characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of rubber antibacterial function detection. Background technique [0002] Rubber products are widely used in the fields of industry and people's livelihood. Although the polymer components in rubber materials and their products do not have the carbon source required for mold growth, they can inhibit the growth of mold; Agents and other substances provide the necessary nutrient source for mold infection, which can easily lead to the decline of its appearance and physical and chemical properties; at the same time, it constitutes a channel for disease transmission. ...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-Determination of antibacterial activity of textile products - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content characterizes the anti-microbial / fungal performance of the fluorescence assay, but it is only suitable for textile or microporous materials with water absorption and the control sample / mycotic growth value> 0, in the key points such as the recovery method of viable bacteria and the effective conditions of the test Technical aspects are not applicable to rubber, plastic and other materials with non-absorbent hard surface and control mold growth value <0, and no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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