Method for detecting mold resistance of antibacterial paint by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a detection method for mold resistance of antibacterial paint. The detection method comprises the detection steps of performing sample extraction, preparing and preprocessing; performing equipment selection and preparing a reagent and a culture medium; preserving and activating strains, and preparing a bacterium suspension; establishing a standard curve of the OD value-viable bacteria content log lgC, and performing calibration on C of inoculated bacterial liquid; establishing a standard curve of a lgC-relative fluorescence intensity log lgI ; performing sample inoculating, culturing, eluting and recovering; performing IB determination of the recovered liquid, and reckoning the viable bacteria content C and T; calculating antibacterial rate R or antibacterial activity value A; and performing result evaluation. The method has the characteristics that the mold resistance, expressed by R or A, of the paint is subjected to pinpoint quantitative test through an ATP fluorophotometer. Control samples and antibacterial samples after contact for 0h and culture for 48h are eluted and recovered according to the specification of the invention, the I of recovered liquid is measured and expressed with lgI, and R or A is calculated. A result assessment basis is provided. The ATP biofluorescence lgC-lgI standard curve method for detecting mold resistance of the antibacterial paint, which is researched and developed by the invention, supports improvement of product quality by a scientific and advanced detection technique.

Description

technical field [0001] The invention relates to a method for testing the mildew resistance of antibacterial coatings, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the mildew resistance of antibacterial coatings characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of paint antibacterial function detection. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, the indoor environment and the quality of home decoration have attracted much attention, so antibacterial coatings have emerged at the historic moment and the supply is in short supply; according to statistics, the global production of antibacterial coatings in 2017 has exceeded 400,000 tons; the relevant organization Zion Research's latest research report shows that the international antibact...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles- Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity oftextile products. Fluorescence measurement method to characterize anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0, in key technologies such as viable bacteria recovery method and effective test conditions Aspects are not applicable to coating floor materials such as building materials and metals with non-absorbent hard surfaces and control sample bacterial growth value <0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

View more