Method for detecting anti-fungal performance of coated antibacterial glass by ATP bioluminescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting anti-fungal performance of coated antibacterial glass. The method for detecting the anti-fungal performance of the coated antibacterial glass comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation and excitation and bacterium suspension preparation are carried out; viable bacterium content C blood cell plate counting of a bacterium suspension and inoculating bacterium solution C calibration after methylene blue staining are carried out; an lgC-relative fluorescence intensity log value lgI standard curve is established; sample inoculating, cultivating, eluting and recycling are carried out; recycled solution I measurement and viable bacterium content C and T calculation of a recycled solution are carried out; the antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the anti-fungal performance of the coated antibacterial glass is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the glass anti-fungal performance represented by R or A. According to the method for detecting the anti-fungal performance of the coated antibacterial glass, it is stipulated that control samples and antibacterial samples are recovered by elution after contact for 0h and cultivating for 24h / 48h and recycled, and a recycling solution I is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting anti-fungal performance of the coated antibacterial glass, an advanced detection technology can be used for supporting product quality improvement.

Description

technical field [0001] The invention relates to a method for testing the antifungal performance of coated antibacterial glass, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antifungal performance of coated antibacterial glass characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of glass antibacterial function detection. Background technique [0002] As a new type of ecological functional material, antibacterial glass adopts nano "dispersion" and "modification" technology to combine antibacterial agent with glass in a chemical bonding way, which belongs to the highest level of coated glass; it is used in medical care, food preservation, transportation and 3C electronic touch products and other fields are widely used. According to the global market observation report, the antibacterial glass market size in 2017 ...

AI Technical Summary

Problems solved by technology

In view of the current development trend of quantification, rapidity and simplification in the research field of foreign antibacterial material performance testing technology, the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-antibacterial finishing textiles) Determination of Antifungal Activity of Antifungal Activity of Textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorescence determination of bacteria / mold properties, but it is only applicable to textile materials or microporous materials with water absorption and control sample bacteria / mold growth value > 0, and it is not applicable to key technologies such as the recovery method of viable bacteria and the effective conditions of the test Inorganic non-metallic materials such as glass and ceramics with non-absorbent hard surface and control fungal growth value <0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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