Method for detecting mildew-proofing performance of household electric appliances by ATP bio-fluorescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting the mildew-proofing performance of household electric appliances. The method comprises the following detection steps: preparing and pre-treating a sample; selecting equipment models and preparing a reagent and a culture medium; preserving and activating strains and preparing a spore solution; establishing an OD value-viable content logarithm value lgCB standard curve; establishing an ATP concentration logarithm value lgCA-relative fluorescence intensity logarithm value lgIA standard curve and calibrating viable bacteria CA of an inoculating bacterial solution; performing sample inoculation, cultivation and eluting reclamation; detecting IA of reclaimed solution and estimating CA and TA of ATP concentration of living bacteria of the reclaimed solution; calculating an antibacterial ratio R or antibacterial activity A; and evaluating the result. The method is characterized in that the R or A represented mildew-proofing performance of household electric appliances can be accurately and quantitatively tested by applying an ATP fluorophotometer. According to the method, a control sample and an antibacterial sample after 0 hour of elution reclamation contact and 48 hours of cultivation are specified, the IA of the reclaimed solution is detected, and R or A is represented and calculated by lgIA. According to the ATP bio-fluorescence lgCA-lgIA standard curve method for detecting the mildew-proofing performance of household electric appliances, product quality improvement is supported by scientific and advanced detection techniques.

Description

technical field [0001] The invention relates to a method for testing the anti-mold performance of household appliances, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the anti-mold performance of household appliances characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of antibacterial function detection of household appliances. Background technique [0002] Over the years, the innovation efficiency and market benefits of my country's home appliance industry cannot be underestimated. With the enhancement of people's awareness of health and environmental protection, consumers' antibacterial demand for home appliances is changing with each passing day. At the end of the last century, antibacterial home appliances came into being. The application and popularization of the industry is unstoppable, and the antibacterial ...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field has developed ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles- Determination of Antibacterial Performance of Antibacterial Finished Textiles) and ISO 13629-1:2012 "Textiles-Determination of antifungal activity oftextile products. Fluorometric assay of anti-microbial / mold performance by content change, but it is only applicable to textile or microporous materials with water absorption and control sample / mycotic growth value > 0. It is not applicable to plastics, glass and other household appliance parts materials with non-absorbent hard surface and mold growth value of the control sample < 0, and does not provide measurement uncertainty evaluation

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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